Abstract
Loop-mediated isothermal amplification (LAMP ), a DNA amplification method, has been applied to the detection of various pathogenic organisms because it features rapid reaction time, accuracy, cost-effect and results that can be determined with the naked eye. The most advantage of this method is that DNA amplification occurs at a constant temperature , usually between 60 and 65 °C, therefore sophisticated equipments are unnecessary. It can be easy that this method is employed in resource-limited laboratories and flied. We have designed a LAMP primer set to detect the kinetoplast minicircle DNA of Leishmania donovani . The LAMP was sensitive and could detect 1 fg of L. donovani DNA, and was not cross-reacted with DNA of 5 other Leishmania species, Plasmodium falciparum and human. The LAMP sensitivity and specificity is equal to nested PCR in DNA samples form patients with visceral leishmaniasis (VL). Therefore, the LAMP can be a better alternative to nested PCR, especially under field conditions, and will be a powerful tool for VL mass screening in combination with urine ELISA.
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References
Murray HW, Berman JD, Davies CR, Saravia NG. Advances in leishmaniasis. Lancet 2005;366:1561–77.
World Health Organization. Regional Strategic Framework for Elimination of Kala-azar from the Southeast Asia Region (2011–2015). New Delhi: WHO Regional Office for Southeast Asia 2004. Accessed August 2005, at http://www.searo.who.int/entity/world_health_day/2014/KA_CD239.pdf.
Sundar S, Rai M. Laboratory diagnosis of visceral leishmaniasis. Clin Diagn Lab Immunol. 2002;9:951–8.
Chappuis F, Sundar S, Hailu A, Ghalib H, Rijal S, Peeling RW, Alvar J, Boelaert M. Visceral leishmaniasis: what are the needs for diagnosis, treatment and control? Nat Rev Microbiol. 2007;5:873–82.
Sundar S, Pai K, Sahu M, Kumar V, Murray HW. Immunochromatographic strip-test detection of anti-K39 antibody in Indian visceral leishmaniasis. Ann Trop Med Parasitol. 2002;96:19–23.
Islam MZ, Itoh M, Takagi H, Islam AU, Ekram AR, Rahman A, Takesue A, Hashiguchi Y, Kimura E. Enzyme-linked immunosorbent assay to detect urinary antibody against recombinant rKRP42 antigen made from Leishmania donovani for the diagnosis of visceral leishmaniasis. Am J Trop Med Hyg. 2008;79:599–604.
Attar ZJ, Chance ML, el-Safi S, et al. Latex agglutination test for the detection of urinary antigens in visceral leishmaniasis. Acta Trop. 2001;78:11–6.
Singh OP, Sundar S. Developments in diagnosis of visceral leishmaniasis in the elimination era. J Parasitol Res. 2015:239469.
Antinori S, Calattini S, Longhi E, Bestetti G, Piolini R, Magni C, Orlando G, Gramiccia M, Acquaviva V, Foschi A, Corvasce S, Colomba C, Titone L, Parravicini C, Cascio A, Corbellino M. Clinical use of polymerase chain reaction performed on peripheral blood and bone marrow samples for the diagnosis and monitoring of visceral leishmaniasis in HIV-infected and HIV-uninfected patients: a single-center, 8-year experience in Italy and review of the literature. Clin Infect Dis. 2007;44:1602–10.
Reithinger R, Dujardin JC. Molecular diagnosis of leishmaniasis: current status and future applications. J Clin Microbiol. 2007;45:21–5.
Singh RK, Pandey HP, Sundar S. Visceral leishmaniasis (kala-azar): challenges ahead. Indian J Med Res. 2006;123:331–44.
Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28:E63.
Nagamine K, Hase T, Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes. 2002;16:223–9.
Takagi H, Itoh M, Islam MZ, et al. Sensitive, specific, and rapid detection of Leishmania donovani DNA by loop-mediated isothermal amplification. Am J Trop Med Hyg. 2009;81:578–82.
Salotra P, Sreenivas G, Pogue GP, Lee N, Nakhasi HL, Ramesh V, Negi NS. Development of a species-specific PCR assay for detection of Leishmania donovani in clinical samples from patients with kala-azar and post-kala-azar dermal leishmaniasis. J Clin Microbiol. 2001;39:849–54.
Bern C, Haque R, Chowdhury R, Ali M, Kurkjian KM, Vaz L, Amann J, Wahed MA, Wagatsuma Y. The epidemiology of visceral leishmaniasis and asymptomatic leishmanial infection in a highly endemic Bangladeshi village. Am J Trop Med Hyg. 2007;76(5):909–14.
Ostyn B, Gidwani K, Khanal B, Picado A, Chappuis F, Singh SP, Rijal S, Sundar S, Boelaert M. Incidence of symptomatic and asymptomatic Leishmania donovani infections in high-endemic foci in India and Nepal: a prospective study. PLoS Negl Trop Dis. 2011;5(10):e1284.
Poon LL, Wong BW, Ma EH, Chan KH, Chow LM, Abeyewickreme W, Tangpukdee N, Yuen KY, Guan Y. Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin Chem. 2006;52(2):303–6.
Hayashida K, Kajino K, Hachaambwa L, Namangala B, Sugimoto C. Direct blood dry LAMP: a rapid, stable, and easy diagnostic tool for Human African Trypanosomiasis. PLoS Negl Trop Dis. 2015;9(3):e0003578.
Wilson IG. Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol. 1997;63:3741–51.
Kaneko H, Kawana T, Fukushima E, Suzutani T. Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances. J Biochem Biophys Methods. 2007;70:499–501.
Nzelu CO, Gomez EA, Caceres AG, Sakurai T, Martini-Robles L, Uezato H, Mimori T, Katakura K, Hashiguchi Y, Kato H. Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection. Acta Trop. 2014;132:1–6.
Acknowledgments
Research underlying this chapter, conducted by the authors, was partially supported by JST/JICA, SATREPS, and also by a Grant-in-Aid for Scientific Research (B) No. 18406013 from the Japan Society for the Promotion of Science.
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Takagi, H., Itoh, M., Noiri, E. (2016). Loop-Mediated Isothermal Amplification (LAMP ): Molecular Diagnosis for the Field Survey of Visceral Leishmaniasis. In: Noiri, E., Jha, T. (eds) Kala Azar in South Asia. Springer, Cham. https://doi.org/10.1007/978-3-319-47101-3_14
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