Abstract
Zinc oxide nanoparticles are a broad-spectrum antimicrobial agent. The size and surface area of ZnO nanomaterials are important parameters which makes it superior than other nanomaterials for their antimicrobial action. In the present chapter, biosynthesized ZnO nanoparticles (diameter ranging from 10 to 25 nm) and Cu-doped ZnO nanorods (length ~500 nm, diameter ~50 nm) were used for antimicrobial activity. Samples were characterized by UV–Vis spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD). Antimicrobial activity of samples is studied by shake-flask method and cytotoxicity by MTT assay. The significant antimicrobial activity of nanoparticles had been observed for Escherichia coli, Staphylococcus aureus, and Streptococcus pyogenes. SEM analysis has been carried on Gram-negative as well as Gram-positive bacteria to understand the mechanism underlying the antimicrobial action of nanostructure materials. Antimicrobial activity is studied as a function of nanoparticle concentration, viz. 20, 40, 60, 80, and 100 ppm.
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Khanuja, M., Uma, Varma, A. (2017). Synthesis and Characterization of Pure and Doped ZnO Nanostructures for Antimicrobial Applications: Effect of Dopant Concentration with Their Mechanism of Action. In: Ghorbanpour, M., Manika, K., Varma, A. (eds) Nanoscience and Plant–Soil Systems. Soil Biology, vol 48. Springer, Cham. https://doi.org/10.1007/978-3-319-46835-8_6
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DOI: https://doi.org/10.1007/978-3-319-46835-8_6
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