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Nitrite and Nitrate in Human Health and Disease pp 293–310Cite as

Nitrate and Exercise Performance

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Part of the book series: Nutrition and Health ((NH))

Abstract

Nitrate (NO3 −), which can be increased via dietary supplementation, is a precursor for nitric oxide (NO) synthesis in hypoxic and acidic conditions. Skeletal muscles become acidic and hypoxic during exercise, increasing the potential for NO synthesis after NO3 − supplementation. NO3 − supplementation has been shown to improve exercise economy and endurance performance in moderately trained, but not well-trained subjects. More recently, NO3 − supplementation has been shown to improve short-duration, high-intensity exercise performance. Overall, NO3 − supplementation has significant potential to enhance exercise performance and this review summarizes the NO3 − supplementation procedures, exercise settings and subject groups in which performance enhancement is most likely.

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References

  1. Gilchrist M, Winyard PG, Benjamin N. Dietary nitrate—good or bad? Nitric Oxide. 2010;22(2):104–9.

    Article  CAS  PubMed  Google Scholar 

  2. Hord NG, Tang Y, Bryan NS. Food sources of nitrates and nitrites: the physiologic context for potential health benefits. Am J Clin Nutr. 2009;90(1):1–10.

    Article  CAS  PubMed  Google Scholar 

  3. EFSA. Nitrate in vegetables: scientific opinion of the panel on contaminants in the food chain. EFSA J. 2008;689:1–79.

    Google Scholar 

  4. Ysart G, Miller P, Barrett G, Farrington D, Lawrance P, Harrison N. Dietary exposures to nitrate in the UK. Food Addit Contam. 1999;16(12):521–32.

    Article  CAS  PubMed  Google Scholar 

  5. Appel LJ, Moore TJ, Obarzanek E, Vollmer WM, Svetkey LP, Sacks FM, Bray GA, Vogt TM, Cutler JA, Windhauser MM, Lin PH, Karanja N. A clinical trial of the effects of dietary patterns on blood pressure. DASH Collaborative Research Group. N Engl J Med. 1997;336(16):1117–24.

    Article  CAS  PubMed  Google Scholar 

  6. NHS Choices. Why 5 a day? 2013. http://www.nhs.uk/Livewell/5ADAY/Pages/5ADAYhome.aspx. Accessed 25 Aug 2015.

  7. EUFIC. Fruit and vegetable consumption in Europe—do Europeans get enough? 2012. http://www.eufic.org/article/en/expid/Fruit-vegetable-consumption-Europe/. Accessed 25 Aug 2015.

  8. Kapil V, Webb AJ, Ahluwalia A. Inorganic nitrate and the cardiovascular system. Heart. 2010;96(21):1703–9.

    Article  CAS  PubMed  Google Scholar 

  9. Omar SA, Webb AJ. Nitrite reduction and cardiovascular protection. J Mol Cell Cardiol. 2014;73:57–69.

    Article  CAS  PubMed  Google Scholar 

  10. Ashworth A, Mitchell K, Blackwell JR, Vanhatalo A, Jones AM. High-nitrate vegetable diet increases plasma nitrate and nitrite concentrations and reduces blood pressure in healthy women. Public Health Nutr. 2015;18:2669–78.

    Article  PubMed  Google Scholar 

  11. Joshipura KJ, Ascherio A, Manson JE, Stampfer MJ, Rimm EB, Speizer FE, et al. Fruit and vegetable intake in relation to risk of ischemic stroke. JAMA. 1999;282:1233–9.

    Article  CAS  PubMed  Google Scholar 

  12. Joshipura KJ, Hu FB, Manson JE, Stampfer MJ, Rimm EB, Speizer FE, et al. The effect of fruit and vegetable intake on risk for coronary heart disease. Ann Intern Med. 2001;134:1106–14.

    Article  CAS  PubMed  Google Scholar 

  13. Omar SA, Webb AJ, Lundberg JO, Weitzberg E. Therapeutic effects of inorganic nitrate and nitrite in cardiovascular and metabolic diseases. J Intern Med. 2016;279:315–36.

    Article  CAS  PubMed  Google Scholar 

  14. Weitzberg E, Lundberg JO. Novel aspects of dietary nitrate and human health. Annu Rev Nutr. 2013;33:129–59.

    Article  CAS  PubMed  Google Scholar 

  15. Spiegelhalder B, Eisenbrand G, Preussmann R. Influence of dietary nitrate on nitrite content of human saliva: possible relevance to in vivo formation of N-nitroso compounds. Food Cosmet Toxicol. 1976;14:545–8.

    Article  CAS  PubMed  Google Scholar 

  16. Govoni M, Jansson EA, Weitzberg E, Lundberg JO. The increase in plasma nitrite after a dietary nitrate load is markedly attenuated by an antibacterial mouthwash. Nitric Oxide. 2008;19:333–7.

    Article  CAS  PubMed  Google Scholar 

  17. Lundberg JO, Govoni M. Inorganic nitrate is a possible source for systemic generation of nitric oxide. Free Radic Biol Med. 2004;37:395–400.

    Article  CAS  PubMed  Google Scholar 

  18. Duncan C, Dougall H, Johnston P, Green S, Brogan R, Leifert C, et al. Chemical generation of nitric oxide in the mouth from the enterosalivary circulation of dietary nitrate. Nat Med. 1995;1:546–51.

    Article  CAS  PubMed  Google Scholar 

  19. Hyde ER, Andrade F, Vaksman Z, Parthasarathy K, Jiang H, Parthasarathy DK, et al. Metagenomic analysis of nitrate-reducing bacteria in the oral cavity: implications for nitric oxide homeostasis. PLoS One. 2014;9, e88645.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  20. Hyde ER, Luk B, Cron S, Kusic L, McCue T, Bauch T, et al. Characterization of the rat oral microbiome and the effects of dietary nitrate. Free Radic Biol Med. 2014;77:249–57.

    Article  CAS  PubMed  Google Scholar 

  21. Lundberg JO, Weitzberg E, Cole JA, Benjamin N. Nitrate, bacteria and human health. Nat Rev Microbiol. 2004;2:593–602.

    Article  CAS  PubMed  Google Scholar 

  22. Benjamin N, O’Driscoll F, Dougall H, Duncan C, Smith L, Golden M, et al. Stomach NO synthesis. Nature. 1994;368:502.

    Article  CAS  PubMed  Google Scholar 

  23. Lundberg JO, Weitzberg E, Lundberg JM, Alving K. Intragastric nitric oxide production in humans: measurements in expelled air. Gut. 1994;35:1543–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. van Faassen EE, Bahrami S, Feelisch M, Hogg N, Kelm M, Kim-Shapiro DB, et al. Nitrite as regulator of hypoxic signaling in mammalian physiology. Med Res Rev. 2009;29:683–741.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  25. Bryan NS, Calvert JW, Gundewar S, Lefer DJ. Dietary nitrite restores NO homeostasis and is cardioprotective in endothelial nitric oxide synthase-deficient mice. Free Radic Biol Med. 2008;45:468–74.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Carlström M, Larsen FJ, Nyström T, Hezel M, Borniquel S, Weitzberg E, et al. Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice. Proc Natl Acad Sci U S A. 2010;107:17716–20.

    Article  PubMed  PubMed Central  Google Scholar 

  27. Ferguson SK, Glean AA, Holdsworth CT, Wright JL, Fees AJ, Colburn TD, et al. Skeletal muscle vascular control during exercise: impact of nitrite infusion during nitric oxide synthase inhibition in healthy rats. J Cardiovasc Pharmacol Ther. 2016;21:201–8.

    Article  CAS  PubMed  Google Scholar 

  28. Lundberg JO, Weitzberg E, Gladwin MT. The nitrate-nitrite-nitric oxide pathway in physiology and therapeutics. Nat Rev Drug Discov. 2008;7:156–67.

    Article  CAS  PubMed  Google Scholar 

  29. Richardson RS, Noyszewski EA, Kendrick KF, Leigh JS, Wagner PD. Myoglobin O2 desaturation during exercise. Evidence of limited O2 transport. J Clin Invest. 1995;96:1916–26.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Tanaka Y, Poole DC, Kano Y. pH homeostasis in contracting and recovering skeletal muscle: integrated function of the microcirculation with the interstitium and intramyocyte milieu. Curr Top Med Chem. 2016;16:2656–63.

    Article  CAS  PubMed  Google Scholar 

  31. Ferguson SK, Holdsworth CT, Wright JL, Fees AJ, Allen JD, Jones AM, et al. Microvascular oxygen pressures in muscles comprised of different fiber types: impact of dietary nitrate supplementation. Nitric Oxide. 2015;48:38–43.

    Article  CAS  PubMed  Google Scholar 

  32. Castello PR, David PS, McClure T, Crook Z, Poyton RO. Mitochondrial cytochrome oxidase produces nitric oxide under hypoxic conditions: implications for oxygen sensing and hypoxic signaling in eukaryotes. Cell Metab. 2006;3:277–87.

    Article  CAS  PubMed  Google Scholar 

  33. Modin A, Björne H, Herulf M, Alving K, Weitzberg E, Lundberg JO. Nitrite-derived nitric oxide: a possible mediator of ‘acidic-metabolic’ vasodilation. Acta Physiol Scand. 2001;171:9–16.

    CAS  PubMed  Google Scholar 

  34. Gladwin MT, Shelhamer JH, Schechter AN, Pease-Fye ME, Waclawiw MA, et al. Role of circulating nitrite and S-nitrosohemoglobin in the regulation of regional blood flow in humans. Proc Natl Acad Sci U S A. 2000;97:11482–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Stamler JS, Meissner G. Physiology of nitric oxide in skeletal muscle. Physiol Rev. 2001;81:209–37.

    CAS  PubMed  Google Scholar 

  36. Suhr F, Gehlert S, Grau M, Bloch W. Skeletal muscle function during exercise-fine-tuning of diverse subsystems by nitric oxide. Int J Mol Sci. 2013;14:7109–39.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Bryan NS. Nitrite in nitric oxide biology: cause or consequence? A systems-based review. Free Radic Biol Med. 2006;41:691–701.

    Article  CAS  PubMed  Google Scholar 

  38. Larsen FJ, Weitzberg E, Lundberg JO, Ekblom B. Effects of dietary nitrate on oxygen cost during exercise. Acta Physiol (Oxf). 2007;191:59–66.

    Article  CAS  Google Scholar 

  39. Poole DC, Richardson RS. Determinants of oxygen uptake. Implications for exercise testing. Sports Med. 1997;24:308–20.

    Article  CAS  PubMed  Google Scholar 

  40. Jones AM, Carter H. The effect of endurance training on parameters of aerobic fitness. Sports Med. 2000;29:373–86.

    Article  CAS  PubMed  Google Scholar 

  41. Bailey SJ, Winyard P, Vanhatalo A, Blackwell JR, Dimenna FJ, Wilkerson DP, et al. Dietary nitrate supplementation reduces the O2 cost of low-intensity exercise and enhances tolerance to high-intensity exercise in humans. J Appl Physiol. 2009;107:1144–55.

    Article  CAS  PubMed  Google Scholar 

  42. Betteridge S, Bescós R, Martorell M, Pons A, Garnham AP, Stathis CC, et al. No effect of acute beetroot juice ingestion on oxygen consumption, glucose kinetics, or skeletal muscle metabolism during submaximal exercise in males. J Appl Physiol. 2016;120:391–8.

    Article  CAS  PubMed  Google Scholar 

  43. Larsen FJ, Weitzberg E, Lundberg JO, Ekblom B. Dietary nitrate reduces maximal oxygen consumption while maintaining work performance in maximal exercise. Free Radic Biol Med. 2010;48:342–7.

    Article  CAS  PubMed  Google Scholar 

  44. Vanhatalo A, Bailey SJ, Blackwell JR, DiMenna FJ, Pavey TG, Wilkerson DP, et al. Acute and chronic effects of dietary nitrate supplementation on blood pressure and the physiological responses to moderate-intensity and incremental exercise. Am J Physiol Regul Integr Comp Physiol. 2010;299:1121–31.

    Article  CAS  Google Scholar 

  45. Wylie LJ, Kelly J, Bailey SJ, Blackwell JR, Skiba PF, Winyard PG, et al. Beetroot juice and exercise: pharmacodynamic and dose-response relationships. J Appl Physiol. 2013;115:325–36.

    Article  CAS  PubMed  Google Scholar 

  46. Wylie LJ, Ortiz de Zevallos J, Isidore T, Nyman L, Vanhatalo A, et al. Dose-dependent effects of dietary nitrate on the oxygen cost of moderate-intensity exercise: Acute vs. chronic supplementation. Nitric Oxide. 2016;57:30–9.

    Article  CAS  PubMed  Google Scholar 

  47. Omar SA, Artime E, Webb AJ. A comparison of organic and inorganic nitrates/nitrites. Nitric Oxide. 2012;26:229–40.

    Article  CAS  PubMed  Google Scholar 

  48. Breese BC, McNarry MA, Marwood S, Blackwell JR, Bailey SJ, Jones AM. Beetroot juice supplementation speeds O2 uptake kinetics and improves exercise tolerance during severe-intensity exercise initiated from an elevated metabolic rate. Am J Physiol Regul Integr Comp Physiol. 2013;305:1441–50.

    Article  CAS  Google Scholar 

  49. Kelly J, Vanhatalo A, Bailey SJ, Wylie LJ, Tucker C, List S, et al. Dietary nitrate supplementation: effects on plasma nitrite and pulmonary O2 uptake dynamics during exercise in hypoxia and normoxia. Am J Physiol Regul Integr Comp Physiol. 2014;307:920–30.

    Article  CAS  Google Scholar 

  50. Lundberg JO, Weitzberg E. NO-synthase independent NO generation in mammals. Biochem Biophys Res Commun. 2010;396:39–45.

    Article  CAS  PubMed  Google Scholar 

  51. McDonagh ST, Wylie LJ, Winyard PG, Vanhatalo A, Jones AM. The effects of chronic nitrate supplementation and the use of strong and weak antibacterial agents on plasma nitrite concentration and exercise blood pressure. Int J Sports Med. 2015;36:1177–85.

    Article  CAS  PubMed  Google Scholar 

  52. Woessner M, Smoliga JM, Tarzia B, Stabler T, Van Bruggen M, Allen JD. A stepwise reduction in plasma and salivary nitrite with increasing strengths of mouthwash following a dietary nitrate load. Nitric Oxide. 2016;54:1–7.

    Article  CAS  PubMed  Google Scholar 

  53. Bailey SJ, Fulford J, Vanhatalo A, Winyard PG, Blackwell JR, DiMenna FJ, et al. Dietary nitrate supplementation enhances muscle contractile efficiency during knee-extensor exercise in humans. J Appl Physiol. 2010;109:135–48.

    Article  CAS  PubMed  Google Scholar 

  54. Lansley KE, Winyard PG, Fulford J, Vanhatalo A, Bailey SJ, Blackwell JR, et al. Dietary nitrate supplementation reduces the O2 cost of walking and running: a placebo-controlled study. J Appl Physiol. 2011;110:591–600.

    Article  CAS  PubMed  Google Scholar 

  55. Kuennen M, Jansen L, Gillum T, Granados J, Castillo W, Nabiyar A, et al. Dietary nitrate reduces the O2 cost of desert marching but elevates the rise in core temperature. Eur J Appl Physiol. 2015;115:2557–69.

    Article  CAS  PubMed  Google Scholar 

  56. Murphy M, Eliot K, Heuertz RM, Weiss E. Whole beetroot consumption acutely improves running performance. J Acad Nutr Diet. 2012;112:548–52.

    Article  PubMed  Google Scholar 

  57. Porcelli S, Ramaglia M, Bellistri G, Pavei G, Pugliese L, Montorsi M, et al. Aerobic fitness affects the exercise performance responses to nitrate supplementation. Med Sci Sports Exerc. 2015;47:1643–51.

    Article  CAS  PubMed  Google Scholar 

  58. Muggeridge DJ, Howe CC, Spendiff O, Pedlar C, James PE, Easton C. The effects of a single dose of concentrated beetroot juice on performance in trained flatwater kayakers. Int J Sport Nutr Exerc Metab. 2013;23:498–506.

    Article  CAS  PubMed  Google Scholar 

  59. Peeling P, Cox GR, Bullock N, Burke LM. Beetroot juice improves on-water 500 M time-trial performance, and laboratory-based paddling economy in national and international-level kayak athletes. Int J Sport Nutr Exerc Metab. 2015;25:278–84.

    Article  PubMed  Google Scholar 

  60. Bond H, Morton L, Braakhuis AJ. Dietary nitrate supplementation improves rowing performance in well-trained rowers. Int J Sport Nutr Exerc Metab. 2012;22:251–6.

    Article  CAS  PubMed  Google Scholar 

  61. Hoon MW, Jones AM, Johnson NA, Blackwell JR, Broad EM, Lundy B, et al. The effect of variable doses of inorganic nitrate-rich beetroot juice on simulated 2,000-m rowing performance in trained athletes. Int J Sports Physiol Perform. 2014;9:615–20.

    Article  PubMed  Google Scholar 

  62. Cermak NM, Res P, Stinkens R, Lundberg JO, Gibala MJ, van Loon LJ. No improvement in endurance performance after a single dose of beetroot juice. Int J Sport Nutr Exerc Metab. 2012;22:470–8.

    Article  CAS  PubMed  Google Scholar 

  63. Lansley KE, Winyard PG, Bailey SJ, Vanhatalo A, Wilkerson DP, Blackwell JR, et al. Acute dietary nitrate supplementation improves cycling time trial performance. Med Sci Sports Exerc. 2011;43:1125–31.

    Article  CAS  PubMed  Google Scholar 

  64. Cermak NM, Gibala MJ, van Loon LJ. Nitrate supplementation’s improvement of 10-km time-trial performance in trained cyclists. Int J Sport Nutr Exerc Metab. 2012;22:64–71.

    Article  CAS  PubMed  Google Scholar 

  65. Flueck JL, Bogdanova A, Mettler S, Perret C. Is beetroot juice more effective than sodium nitrate? The effects of equimolar nitrate dosages of nitrate-rich beetroot juice and sodium nitrate on oxygen consumption during exercise. Appl Physiol Nutr Metab. 2016;41:421–9.

    Article  CAS  PubMed  Google Scholar 

  66. Shepherd AI, Gilchrist M, Winyard PG, Jones AM, Hallmann E, Kazimierczak R, et al. Effects of dietary nitrate supplementation on the oxygen cost of exercise and walking performance in individuals with type 2 diabetes: a randomized, double-blind, placebo-controlled crossover trial. Free Radic Biol Med. 2015;86:200–8.

    Article  CAS  PubMed  Google Scholar 

  67. Wootton-Beard PC, Ryan L. A beetroot juice shot is a significant and convenient source of bioaccessible antioxidants. J Funct Foods. 2011;3:329–34.

    Article  CAS  Google Scholar 

  68. Brown GC. Control of respiration and ATP synthesis in mammalian mitochondria and cells. Biochem J. 1992;284:1–13.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  69. Chance B, Williams GR. Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem. 1955;217:383–93.

    CAS  PubMed  Google Scholar 

  70. Allen DG, Lamb GD, Westerblad H. Skeletal muscle fatigue: cellular mechanisms. Physiol Rev. 2008;88:287–332.

    Article  CAS  PubMed  Google Scholar 

  71. Larsen FJ, Schiffer TA, Borniquel S, Sahlin K, Ekblom B, Lundberg JO, et al. Dietary inorganic nitrate improves mitochondrial efficiency in humans. Cell Metab. 2011;13:149–59.

    Article  CAS  PubMed  Google Scholar 

  72. Larsen FJ, Schiffer TA, Ekblom B, Mattsson MP, Checa A, Wheelock CE, et al. Dietary nitrate reduces resting metabolic rate: a randomized, crossover study in humans. Am J Clin Nutr. 2014;99:843–50.

    Article  CAS  PubMed  Google Scholar 

  73. Whitfield J, Ludzki A, Heigenhauser GJ, Senden JM, Verdijk LB, van Loon LJ, et al. Beetroot juice supplementation reduces whole body oxygen consumption but does not improve indices of mitochondrial efficiency in human skeletal muscle. J Physiol. 2016;594:421–35.

    Article  CAS  PubMed  Google Scholar 

  74. Hernández A, Schiffer TA, Ivarsson N, Cheng AJ, Bruton JD, Lundberg JO, et al. Dietary nitrate increases tetanic [Ca2+]i and contractile force in mouse fast-twitch muscle. J Physiol. 2012;590:3575–83.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  75. Barclay CJ, Woledge RC, Curtin NA. Energy turnover for Ca2+ cycling in skeletal muscle. J Muscle Res Cell Motil. 2007;28:259–74.

    Article  CAS  PubMed  Google Scholar 

  76. Ferguson SK, Hirai DM, Copp SW, Holdsworth CT, Allen JD, Jones AM, et al. Impact of dietary nitrate supplementation via beetroot juice on exercising muscle vascular control in rats. J Physiol. 2013;591:547–57.

    Article  CAS  PubMed  Google Scholar 

  77. Poole DC, Behnke BJ, Padilla DJ. Dynamics of muscle microcirculatory oxygen exchange. Med Sci Sports Exerc. 2005;37:1559–66.

    Article  CAS  PubMed  Google Scholar 

  78. Henneman E, Somjen G, Carpenter DO. Excitability and inhibitability of motoneurons of different sizes. J Neurophysiol. 1965;28:599–620.

    CAS  PubMed  Google Scholar 

  79. Koga S, Kano Y, Barstow TJ, Ferreira LF, Ohmae E, Sudo M, et al. Kinetics of muscle deoxygenation and microvascular PO2 during contractions in rat: comparison of optical spectroscopy and phosphorescence-quenching techniques. J Appl Physiol. 2012;112:26–32.

    Article  CAS  PubMed  Google Scholar 

  80. Coyle EF, Costill DL, Lesmes GR. Leg extension power and muscle fiber composition. Med Sci Sports. 1979;11:12–5.

    CAS  PubMed  Google Scholar 

  81. Coggan AR, Leibowitz JL, Kadkhodayan A, Thomas DP, Ramamurthy S, Spearie CA, et al. Effect of acute dietary nitrate intake on maximal knee extensor speed and power in healthy men and women. Nitric Oxide. 2015;48:16–21.

    Article  CAS  PubMed  Google Scholar 

  82. Bailey SJ, Varnham RL, DiMenna FJ, Breese BC, Wylie LJ, Jones AM. Inorganic nitrate supplementation improves muscle oxygenation, O2 uptake kinetics, and exercise tolerance at high but not low pedal rates. J Appl Physiol. 2015;118:1396–405.

    Article  CAS  PubMed  Google Scholar 

  83. McDonough P, Behnke BJ, Padilla DJ, Musch TI, Poole DC. Control of microvascular oxygen pressures in rat muscles comprised of different fibre types. J Physiol. 2005;563:903–13.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  84. Wilkerson DP, Hayward GM, Bailey SJ, Vanhatalo A, Blackwell JR, Jones AM. Influence of acute dietary nitrate supplementation on 50 mile time trial performance in well-trained cyclists. Eur J Appl Physiol. 2012;112:4127–34.

    Article  CAS  PubMed  Google Scholar 

  85. Boorsma RK, Whitfield J, Spriet LL. Beetroot juice supplementation does not improve performance of elite 1500-m runners. Med Sci Sports Exerc. 2014;46:2326–34.

    Article  CAS  PubMed  Google Scholar 

  86. Peacock O, Tjønna AE, James P, Wisløff U, Welde B, Böhlke N, et al. Dietary nitrate does not enhance running performance in elite cross-country skiers. Med Sci Sports Exerc. 2012;44:2213–9.

    Article  CAS  PubMed  Google Scholar 

  87. Hoon MW, Hopkins WG, Jones AM, Martin DT, Halson SL, West NP, et al. Nitrate supplementation and high-intensity performance in competitive cyclists. Appl Physiol Nutr Metab. 2014;39:1043–9.

    Article  CAS  PubMed  Google Scholar 

  88. Lane SC, Hawley JA, Desbrow B, Jones AM, Blackwell JR, Ross ML, et al. Single and combined effects of beetroot juice and caffeine supplementation on cycling time trial performance. Appl Physiol Nutr Metab. 2014;39:1050–7.

    Article  CAS  PubMed  Google Scholar 

  89. MacLeod KE, Nugent SF, Barr SI, Koehle MS, Sporer BC, MacInnis MJ. Acute beetroot juice supplementation does not improve cycling performance in normoxia or moderate hypoxia. Int J Sport Nutr Exerc Metab. 2015;25:359–66.

    Article  PubMed  Google Scholar 

  90. Bescós R, Ferrer-Roca V, Galilea PA, Roig A, Drobnic F, Sureda A, et al. Sodium nitrate supplementation does not enhance performance of endurance athletes. Med Sci Sports Exerc. 2012;44:2400–9.

    Article  PubMed  CAS  Google Scholar 

  91. Christensen PM, Nyberg M, Bangsbo J. Influence of nitrate supplementation on VO2 kinetics and endurance of elite cyclists. Scand J Med Sci Sports. 2013;23:e21–31.

    Article  CAS  PubMed  Google Scholar 

  92. Green DJ, Maiorana A, O’Driscoll G, Taylor R. Effect of exercise training on endothelium-derived nitric oxide function in humans. J Physiol. 2004;561:1–25.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  93. McConell GK, Bradley SJ, Stephens TJ, Canny BJ, Kingwell BA, Lee-Young RS. Skeletal muscle nNOS mu protein content is increased by exercise training in humans. Am J Physiol Regul Integr Comp Physiol. 2007;293:821–8.

    Article  CAS  Google Scholar 

  94. Kinnunen S, Mänttäri S. Specific effects of endurance and sprint training on protein expression of calsequestrin and SERCA in mouse skeletal muscle. J Muscle Res Cell Motil. 2012;33:123–30.

    Article  CAS  PubMed  Google Scholar 

  95. Fernström M, Tonkonogi M, Sahlin K. Effects of acute and chronic endurance exercise on mitochondrial uncoupling in human skeletal muscle. J Physiol. 2004;554:755–63.

    Article  PubMed  CAS  Google Scholar 

  96. Mogensen M, Bagger M, Pedersen PK, Fernström M, Sahlin K. Cycling efficiency in humans is related to low UCP3 content and to type I fibres but not to mitochondrial efficiency. J Physiol. 2006;571:669–81.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  97. Wilson JM, Loenneke JP, Jo E, Wilson GJ, Zourdos MC, Kim JS. The effects of endurance, strength, and power training on muscle fiber type shifting. J Strength Cond Res. 2012;26:1724–9.

    Article  PubMed  Google Scholar 

  98. Gollnick PD, Armstrong RB, Saubert IV CW, Piehl K, Saltin B. Enzyme activity and fiber composition in skeletal muscle of untrained and trained men. J Appl Physiol. 1972;33:312–9.

    CAS  PubMed  Google Scholar 

  99. Polgar J, Johnson MA, Weightman D, Appleton D. Data on fibre size in thirty-six human muscles. An autopsy study. J Neurol Sci. 1973;19:307–18.

    Article  CAS  PubMed  Google Scholar 

  100. Krustrup P, Söderlund K, Mohr M, Bangsbo J. The slow component of oxygen uptake during intense, sub-maximal exercise in man is associated with additional fibre recruitment. Pflugers Arch. 2004;447:855–66.

    Article  CAS  PubMed  Google Scholar 

  101. Wylie LJ, Mohr M, Krustrup P, Jackman SR, Ermιdis G, Kelly J, et al. Dietary nitrate supplementation improves team sport-specific intense intermittent exercise performance. Eur J Appl Physiol. 2013;113:1673–84.

    Article  PubMed  Google Scholar 

  102. Thompson C, Wylie LJ, Fulford J, Kelly J, Black MI, McDonagh ST, et al. Dietary nitrate improves sprint performance and cognitive function during prolonged intermittent exercise. Eur J Appl Physiol. 2015;115:1825–34.

    Article  CAS  PubMed  Google Scholar 

  103. Aucouturier J, Boissière J, Pawlak-Chaouch M, Cuvelier G, Gamelin FX. Effect of dietary nitrate supplementation on tolerance to supramaximal intensity intermittent exercise. Nitric Oxide. 2015;49:16–25.

    Article  CAS  PubMed  Google Scholar 

  104. Martin K, Smee D, Thompson KG, Rattray B. No improvement of repeated-sprint performance with dietary nitrate. Int J Sports Physiol Perform. 2014;9:845–50.

    Article  PubMed  Google Scholar 

  105. Wylie LJ, Bailey SJ, Kelly J, Blackwell JR, Vanhatalo A, Jones AM. Influence of beetroot juice supplementation on intermittent exercise performance. Eur J Appl Physiol. 2016;116:415–25.

    Article  CAS  PubMed  Google Scholar 

  106. King T, Jenkins D, Gabbett T. A time-motion analysis of professional rugby league match-play. J Sports Sci. 2009;27:213–9.

    Article  PubMed  Google Scholar 

  107. Mohr M, Krustrup P, Bangsbo J. Match performance of high-standard soccer players with special reference to development of fatigue. J Sports Sci. 2003;21:519–28.

    Article  PubMed  Google Scholar 

  108. Spencer M, Lawrence S, Rechichi C, Bishop D, Dawson B, Goodman C. Time-motion analysis of elite field hockey, with special reference to repeated-sprint activity. J Sports Sci. 2004;22:843–50.

    Article  PubMed  Google Scholar 

  109. Haider G, Folland JP. Nitrate supplementation enhances the contractile properties of human skeletal muscle. Med Sci Sports Exerc. 2014;46:2234–43.

    Article  CAS  PubMed  Google Scholar 

  110. Sandbakk SB, Sandbakk Ø, Peacock O, James P, Welde B, Stokes K, et al. Effects of acute supplementation of L-arginine and nitrate on endurance and sprint performance in elite athletes. Nitric Oxide. 2015;48:10–5.

    Article  CAS  PubMed  Google Scholar 

  111. Rimer EG, Peterson LR, Coggan AR, Martin JC. Acute dietary nitrate supplementation increases maximal cycling power in athletes. Int J Sports Physiol Perform. 2016;11:715–20.

    Google Scholar 

  112. Abernethy PJ, Thayer R, Taylor AW. Acute and chronic responses of skeletal muscle to endurance and sprint exercise. A review. Sports Med. 1990;10:365–89.

    Article  CAS  PubMed  Google Scholar 

  113. Masschelein E, Van Thienen R, Wang X, Van Schepdael A, Thomis M, Hespel P. Dietary nitrate improves muscle but not cerebral oxygenation status during exercise in hypoxia. J Appl Physiol. 2012;113:736–45.

    Article  CAS  PubMed  Google Scholar 

  114. Muggeridge DJ, Howe CC, Spendiff O, Pedlar C, James PE, Easton C. A single dose of beetroot juice enhances cycling performance in simulated altitude. Med Sci Sports Exerc. 2014;46:143–50.

    Article  CAS  PubMed  Google Scholar 

  115. Vanhatalo A, Fulford J, Bailey SJ, Blackwell JR, Winyard PG, Jones AM. Dietary nitrate reduces muscle metabolic perturbation and improves exercise tolerance in hypoxia. J Physiol. 2011;589:5517–28.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  116. Hoon MW, Fornusek C, Chapman PG, Johnson NA. The effect of nitrate supplementation on muscle contraction in healthy adults. Eur J Sport Sci. 2015;15:712–9.

    Article  PubMed  Google Scholar 

  117. Arnold JT, Oliver SJ, Lewis-Jones TM, Wylie LJ, Macdonald JH. Beetroot juice does not enhance altitude running performance in well-trained athletes. Appl Physiol Nutr Metab. 2015;40:590–5.

    Article  CAS  PubMed  Google Scholar 

  118. Bourdillon N, Fan JL, Uva B, Müller H, Meyer P, Kayser B. Effect of oral nitrate supplementation on pulmonary hemodynamics during exercise and time trial performance in normoxia and hypoxia: a randomized controlled trial. Front Physiol. 2015;6:288.

    Article  PubMed  PubMed Central  Google Scholar 

  119. Carriker CR, Mermier CM, McLain TA, Johnson KE, Beltz NM, Vaughan RA, et al. Effect of acute dietary nitrate consumption on oxygen consumption during submaximal exercise in hypobaric hypoxia. Int J Sport Nutr Exerc Metab. 2016;26:315–22.

    Article  PubMed  Google Scholar 

  120. Muggeridge DJ, Sculthorpe N, Grace FM, Willis G, Thornhill L, Weller RB, et al. Acute whole body UVA irradiation combined with nitrate ingestion enhances time trial performance in trained cyclists. Nitric Oxide. 2015;48:3–9.

    Article  CAS  PubMed  Google Scholar 

  121. Askew CD, Green S, Walker PJ, Kerr GK, Green AA, Williams AD, et al. Skeletal muscle phenotype is associated with exercise tolerance in patients with peripheral arterial disease. J Vasc Surg. 2005;41:802–7.

    Article  PubMed  Google Scholar 

  122. Gosker HR, Wouters EF, van der Vusse GJ, Schols AM. Skeletal muscle dysfunction in chronic obstructive pulmonary disease and chronic heart failure: underlying mechanisms and therapy perspectives. Am J Clin Nutr. 2000;71:1033–47.

    CAS  PubMed  Google Scholar 

  123. Mador MJ, Bozkanat E. Skeletal muscle dysfunction in chronic obstructive pulmonary disease. Respir Res. 2001;2:216–24.

    Article  CAS  PubMed  Google Scholar 

  124. Oberbach A, Bossenz Y, Lehmann S, Niebauer J, Adams V, Paschke R, et al. Altered fiber distribution and fiber-specific glycolytic and oxidative enzyme activity in skeletal muscle of patients with type 2 diabetes. Diabetes Care. 2006;29:895–900.

    Article  CAS  PubMed  Google Scholar 

  125. Raguso CA, Guinot SL, Janssens JP, Kayser B, Pichard C. Chronic hypoxia: common traits between chronic obstructive pulmonary disease and altitude. Curr Opin Clin Nutr Metab Care. 2004;7:411–7.

    Article  CAS  PubMed  Google Scholar 

  126. Schaufelberger M, Eriksson BO, Grimby G, Held P, Swedberg K. Skeletal muscle fiber composition and capillarization in patients with chronic heart failure: relation to exercise capacity and central hemodynamics. J Card Fail. 1995;1:267–72.

    Article  CAS  PubMed  Google Scholar 

  127. Kerley CP, Cahill K, Bolger K, McGowan A, Burke C, Faul J, et al. Dietary nitrate supplementation in COPD: an acute, double-blind, randomized, placebo-controlled, crossover trial. Nitric Oxide. 2015;44:105–11.

    Article  CAS  PubMed  Google Scholar 

  128. Berry MJ, Justus NW, Hauser JI, Case AH, Helms CC, Basu S, et al. Dietary nitrate supplementation improves exercise performance and decreases blood pressure in COPD patients. Nitric Oxide. 2015;48:22–30.

    Article  CAS  PubMed  Google Scholar 

  129. Leong P, Basham JE, Yong T, Chazan A, Finlay P, Barnes S, et al. A double blind randomized placebo control crossover trial on the effect of dietary nitrate supplementation on exercise tolerance in stable moderate chronic obstructive pulmonary disease. BMC Pulm Med. 2015;15:52.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  130. Shepherd AI, Wilkerson DP, Dobson L, Kelly J, Winyard PG, Jones AM, et al. The effect of dietary nitrate supplementation on the oxygen cost of cycling, walking performance and resting blood pressure in individuals with chronic obstructive pulmonary disease: a double blind placebo controlled, randomised control trial. Nitric Oxide. 2015;48:31–7.

    Article  CAS  PubMed  Google Scholar 

  131. Kenjale AA, Ham KL, Stabler T, Robbins JL, Johnson JL, Vanbruggen M, et al. Dietary nitrate supplementation enhances exercise performance in peripheral arterial disease. J Appl Physiol. 2011;110:1582–91.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  132. Coggan AR, Leibowitz JL, Spearie CA, Kadkhodayan A, Thomas DP, Ramamurthy S, et al. Acute dietary nitrate intake improves muscle contractile function in patients with heart failure: a double-blind, placebo-controlled, randomized trial. Circ Heart Fail. 2015;8:914–20.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  133. Eggebeen J, Kim-Shapiro DB, Haykowsky M, Morgan TM, Basu S, Brubaker P, et al. One week of daily dosing with beetroot juice improves submaximal endurance and blood pressure in older patients with heart failure and preserved ejection fraction. JACC Heart Fail. 2016. pii: S2213-1779(15)00835-5. doi:10.1016/j.jchf.2015.12.013. [Epub ahead of print].

  134. Zamani P, Rawat D, Shiva-Kumar P, Geraci S, Bhuva R, Konda P, et al. Effect of inorganic nitrate on exercise capacity in heart failure with preserved ejection fraction. Circulation. 2015;131:371–80.

    Article  CAS  PubMed  Google Scholar 

  135. Kelly J, Fulford J, Vanhatalo A, Blackwell JR, French O, Bailey SJ, et al. Effects of short-term dietary nitrate supplementation on blood pressure, O2 uptake kinetics, and muscle and cognitive function in older adults. Am J Physiol Regul Integr Comp Physiol. 2013;304:73–83.

    Article  CAS  Google Scholar 

  136. Clifford T, Bell O, West DJ, Howatson G, Stevenson EJ. The effects of beetroot juice supplementation on indices of muscle damage following eccentric exercise. Eur J Appl Physiol. 2016;116:353–62.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Sport and Health Sciences, College of Life and Environmental Sciences, University of Exeter, St. Luke’s Campus, Heavitree Road, Exeter, EX1 2LU, UK

    Stephen J. Bailey Ph.D., Anni Vanhatalo Ph.D. & Andrew M. Jones Ph.D.

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  1. Stephen J. Bailey Ph.D.
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  2. Anni Vanhatalo Ph.D.
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  3. Andrew M. Jones Ph.D.
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Correspondence to Andrew M. Jones Ph.D. .

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Editors and Affiliations

  1. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA

    Nathan S. Bryan

  2. Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA

    Joseph Loscalzo

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Bailey, S.J., Vanhatalo, A., Jones, A.M. (2017). Nitrate and Exercise Performance. In: Bryan, N., Loscalzo, J. (eds) Nitrite and Nitrate in Human Health and Disease. Nutrition and Health. Humana Press, Cham. https://doi.org/10.1007/978-3-319-46189-2_20

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