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Cryopreservation of Hair-Follicle Associated Pluripotent (HAP) Stem Cells Maintains Differentiation and Hair-Growth Potential

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Biobanking and Cryopreservation of Stem Cells

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 951))

Abstract

Hair follicles contain nestin-expressing pluripotent stem cells which originate above the bulge area of the follicle, below the sebaceous gland. We have termed these cells hair follicle-associated pluripotent (HAP) stem cells. We have established efficient cryopreservation methods of the hair follicle that maintain the pluripotency of HAP stem cells as well as hair growth. We cryopreserved the whole hair follicle by slow-rate cooling in TC-Protector medium or in DMSO-containing medium and storage in liquid nitrogen or at −80 °C. After thawing and culture of the cryopreserved whisker follicles, growing HAP stem cells formed hair spheres. The hair spheres contained cells that differentiated to neurons, glial cells, and other cell types. The hair spheres derived from slow-cooling cryopreserved hair follicles were as pluripotent as hair spheres from fresh hair follicles. We have also previously demonstrated that cryopreserved mouse whisker hair follicles maintain their hair-growth potential. DMSO better cryopreserved mouse whisker follicles compared to glycerol. DMSO-cryopreserved hair follicles also maintained the HAP stem cells, evidenced by P75ntr expression. Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair-shaft growth of cryopreserved hair follicles. HAP stem cells can be used for nerve and spinal-cord repair. This biobanking of hair follicles can allow each patient the potential for their own stem cell use for regenerative medicine or hair transplantation.

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Abbreviations

HAP:

Hair follicle-associated pluripotent

ES:

Embryonic stem

GFP:

Green fluorescent protein

ND-GFP:

Nestin-driven-GFP

SMA:

Smooth muscle actin

GFAP:

Glial fibrillary acidic protein

RT-PCR:

Real-time polymerase chain reaction

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Author information

Authors and Affiliations

  1. AntiCancer, Inc, 7917 Ostrow St, San Diego, CA, 92111, USA

    Robert M. Hoffman & Wenluo Cao

  2. Department of Surgery, University of California, San Diego, CA, USA

    Robert M. Hoffman & Wenluo Cao

  3. Department of Dermatology, Kitasato University, Sagamihara, Japan

    Satoshi Kajiura & Yasuyuki Amoh

  4. Department of Anatomy, Second Military Medical University, Shanghai, China

    Wenluo Cao & Fang Liu

Authors
  1. Robert M. Hoffman
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  2. Satoshi Kajiura
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  3. Wenluo Cao
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  4. Fang Liu
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  5. Yasuyuki Amoh
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Corresponding author

Correspondence to Robert M. Hoffman .

Editor information

Editors and Affiliations

  1. Department of Oncology, University of Alberta, Edmonton, Alberta, Canada

    Feridoun Karimi-Busheri

  2. Department of Oncology, Cross Cancer Institute and University of Alberta, Edmonton, Alberta, Canada

    Michael Weinfeld

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Hoffman, R.M., Kajiura, S., Cao, W., Liu, F., Amoh, Y. (2016). Cryopreservation of Hair-Follicle Associated Pluripotent (HAP) Stem Cells Maintains Differentiation and Hair-Growth Potential. In: Karimi-Busheri, F., Weinfeld, M. (eds) Biobanking and Cryopreservation of Stem Cells. Advances in Experimental Medicine and Biology, vol 951. Springer, Cham. https://doi.org/10.1007/978-3-319-45457-3_16

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