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Cryopreservation of Human Pluripotent Stem Cell-Derived Cardiomyocytes: Strategies, Challenges, and Future Directions

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Book cover Biobanking and Cryopreservation of Stem Cells

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 951))

Abstract

In recent years, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have emerged as a vital cell source for in vitro modeling of genetic cardiovascular disorders, drug screening, and in vivo cardiac regeneration research. Looking forward, the ability to efficiently cryopreserve hPSC-CMs without compromising their normal biochemical and physiologic functions will dramatically facilitate their various biomedical applications. Although working protocols for freezing, storing, and thawing hPSC-CMs have been established, the question remains as to whether they are optimal. In this chapter, we discuss our current understanding of cryopreservation appertaining to hPSC-CMs, and proffer key questions regarding the mechanical, contractile, and regenerative properties of cryopreserved hPSC-CMs.

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Abbreviations

hPSC-CMs:

Human pluripotent stem cell-derived cardiomyocytes

CPA:

Cryoprotective agent

DMSO:

Dimethyl sulfoxide

FBS:

Fetal bovine serum

ROCK:

Rho-associated kinase

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Acknowledgements

This work was supported in part by grants GA-2014-126 from the Center for the Advancement of Science in Space and R21 HL123928 from the NIH. M.K.P. was supported by the Center for Pediatric Nanomedicine at Emory/Georgia Tech. Figures were prepared using Servier Medical Art.

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Authors and Affiliations

  1. Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine and Children’s Healthcare of Atlanta, 2015 Uppergate Drive, Atlanta, GA, 30322, USA

    Marcela K. Preininger, Monalisa Singh & Chunhui Xu

  2. Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA

    Marcela K. Preininger & Chunhui Xu

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  1. Marcela K. Preininger
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  2. Monalisa Singh
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  3. Chunhui Xu
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Correspondence to Chunhui Xu .

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Editors and Affiliations

  1. Department of Oncology, University of Alberta, Edmonton, Alberta, Canada

    Feridoun Karimi-Busheri

  2. Department of Oncology, Cross Cancer Institute and University of Alberta, Edmonton, Alberta, Canada

    Michael Weinfeld

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Preininger, M.K., Singh, M., Xu, C. (2016). Cryopreservation of Human Pluripotent Stem Cell-Derived Cardiomyocytes: Strategies, Challenges, and Future Directions. In: Karimi-Busheri, F., Weinfeld, M. (eds) Biobanking and Cryopreservation of Stem Cells. Advances in Experimental Medicine and Biology, vol 951. Springer, Cham. https://doi.org/10.1007/978-3-319-45457-3_10

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