Abstract
In the present work, we describe the synthesis of zinc oxide nanoparticles (ZnO NPs) using culture supernatant of endophytic bacterial isolate Sphingobacterium thalpophilum and their antibacterial efficiency against bacterial pathogens. In the process of reduction aqueous zinc nitrate being extra-cellular which lead to the development of an easy bioprocess for synthesis of ZnO NPs. The ZnO NPs were characterized by X-ray Powder Diffraction (XRD), Field emission scanning electron microscopy (FESEM ) and Energy dispersive X-ray analysis (EDAX). Ultraviolet-Visible (UV-Vis) Spectrometer and Fourier transfer infrared rays (FTIR) analysis. Antibacterial efficiency of ZnO NPs are tested with two bacterial pathogens Pseudomonas aeruginosa and Enterobacter aerogens using the disc diffusion method to determine their ability as potential antimicrobial agents. This ZnO NPs showed improved antibacterial activity on both tested strains. This was confirmed by zone of inhibition measurements.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Gunalan, S., Sivaraj, R., Rajendran, V.: Green synthesized ZnO nanoparticles against bacterial and fungal pathogens. Prog. Nat. Sci. Mat. Internat. 22, 693–700 (2012)
Wang, A., Lu, J., Xu, M., Xing, B.: Sorption of pyrene by regular and nanoscaled metal oxide particles: influence of adsorbed organic matter. Environ. Sci. Tech. 42, 7267–7272 (2008)
Espitia, P.J.P., Soares, N.F.F., Coimbra, J.S.R., de Andrade, N.J., Cruz, R.S., Medeiros, E.A.A.: Zinc oxide nanoparticles: synthesis, antimicrobial activity and food packaging applications. Food Biopro. Technol. 5, 1447–1464 (2012)
Raghupathi, K.R., Koodali, R.T., Manna, A.C.: Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. Langmuir 27, 4020–4028 (2011)
Magrez, S., Kasas, V., Salicio, N., Pasquier, J., Seo, W., Celio, M., Catsicas, S., Schwaller, B., Forro, L.: Cellular toxicity of carbon-based nanomaterials. Nano Lett. 6, 1121–1125 (2006)
Long, T.C., Saleh, N., Tilton, R.D., Lowry, G.V., Veronesi, B.: Titanium, Dioxide (P25) produces reactive oxygen species in immortalized microglia (Bv2): implications for nanoparticle neurotoxicity. Environ. Sci. Tech. 40, 4346–4352 (2006)
Suyana, P., Nishanth Kumar, S., Dileep Kumar, B.S., Nair, B.N., Pillai, S.C., Peer Mohamed, A., Warriera, K.G.K., Hareesh, U.S.: Antifungal properties of nanosized ZnS particles synthesised by sonochemical precipitation. RSC Adv. 4, 8439–8445 (2014)
Shivaji, S., Madhu, S., Singh, S.: Extracellular synthesis of antibacterial silver nanoparticles using psychrophilic Bacteria. Proc. Bioch. 46, 1800–1807 (2011)
He, S., Guo, Z., Zhang, Y., Zhang, S., Wang, J., Gu, N.: Biosynthesis of gold nanoparticles using the bacteria Rhodopseudomonas capsulate. Mat. Lett. 61, 3984–3987 (2007)
Husseiny, M.I., Abd El-Aziz, M., Badr, Y., Mahmoud, M.A.: Biosynthesis of gold nanoparticles using Pseudomonas aeruginosa. Spect. Acta Part A. 67, 1003–1006 (2007)
Shahverdi, A.R., Minaeian, S., Shahverdi, H.R., Jamalifar, H., Nohi, A.-A.: Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria: a novel biological approach. Proc. Bioch. 42, 919–923 (2007)
Suryanarayana, C., Norton, M.G.: X-ray Diffraction: A Practica Approach. Plenum Press, New York (1998)
Chauhan, R., Kumar, A., Chaudharya, R.P.: Synthesis and characterization of copper dopped ZnO nanoparticles. J. Chem. Pharm. Res. 2, 178–183 (2010)
Sharma, D., Rajput, J., Kaith, B.S., Kaur, M., Sharma, S.: Synthesis of ZnO nanoparticles and study of their antibacterial and antifungal properties. Thin Solid Fil. 519, 1224–1229 (2010)
Zhang, L., Jiang, Y., Ding, Y., Povey, M., York, D.: Investigation into the antibacterial behavior of suspensions of ZnO nanoparticles (ZnO nanofluides). J. Nanopart. Res. 9, 479–489 (2007)
Sunkar, S., Valli Nachiyar, C.: Biogenesis of antibacterial silver nanoparticles using the endophytic bacterium Bacillus cereus isolated from Garcinia xanthochymus. Asian Spac. J Trop. Biomed. 2, 953–959 (2012)
Gordon, T., Perlstein, B., Houbara, O., Felner, I., Banin, E., Margel, S.: Synthesis and characterization of zinc/iron oxide composite nanoparticles and their antibacterial properties. Col. Surf. A. Physicochem. Eng. Aspec. 374, 1–8 (2011)
Becker, J., Raghupathi, K.R., Pierre, J.S., Zhao, D., Koodali, R.T.: Tuning of the crystallite and particle size of ZnO nanocrystallite materials in solvothermal synthesis and their photocatalytic activity for dye degradation. J. Phys. Chem. C 115, 13844–13850 (2011)
Haja Hameed, A.S., Karthikeyan, C., Sasikumar, S., Senthil Kumar, V., Kumaresan, S., Ravi, G.: Impact of alkaline metal ions Mg2+, Ca2+, Sr2+ and Ba2+ on the structural, optical, thermal and antibacterial properties of ZnO nanoparticles prepared by the coprecipitation method. J. Mater. Chem. B. 1, 5950–5962 (2013)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing Switzerland
About this paper
Cite this paper
Rajabairavi, N. et al. (2017). Biosynthesis of Novel Zinc Oxide Nanoparticles (ZnO NPs) Using Endophytic Bacteria Sphingobacterium thalpophilum . In: Ebenezar, J. (eds) Recent Trends in Materials Science and Applications. Springer Proceedings in Physics, vol 189. Springer, Cham. https://doi.org/10.1007/978-3-319-44890-9_23
Download citation
DOI: https://doi.org/10.1007/978-3-319-44890-9_23
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-44889-3
Online ISBN: 978-3-319-44890-9
eBook Packages: Physics and AstronomyPhysics and Astronomy (R0)