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Protein Nitrogen Determination

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Book cover Food Analysis Laboratory Manual

Part of the book series: Food Science Text Series ((FSTS))

Abstract

The protein content of foods can be determined by numerous methods. The Kjeldahl method and the nitrogen combustion (Dumas) method for protein analysis are based on nitrogen determination. Both methods are official for the purposes of nutrition labeling of foods. While the Kjeldahl method has been used widely for over a hundred years, the availability of automated instrumentation for the Dumas method in many cases is replacing use of the Kjeldahl method. This laboratory exercise includes determination of protein by both the Kjeldahl and Dumas methods.

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Resource Materials

  • Chang SKC, Zhang Y (2017) Protein analysis. Ch. 18. In: Nielsen SS (ed) Food analysis, 5th edn. Springer, New York

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  • AOAC International (2016) Official methods of analysis, 20th edn, (On-line). Method 960.52 (Micro-Kjeldahl method) and Method 992.23 (Generic combustion method). AOAC International, Rockville, MD

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Correspondence to S. Suzanne Nielsen .

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© 2017 Springer International Publishing

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Nielsen, S.S. (2017). Protein Nitrogen Determination. In: Food Analysis Laboratory Manual. Food Science Text Series. Springer, Cham. https://doi.org/10.1007/978-3-319-44127-6_13

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