Abstract
β-Glucosidases is known as the terminal enzyme in the synergy with other cellulases for biomass degradation. It completes the final step of small oligosaccharides (including cellobiose) conversion into glucose. However, this is only a small part of the roles played by a subgroup of bacterial/fungal β-glucosidases in cellulose biodegradation. To deepen our understanding about the current challenges and limits in biomass conversion and to enlighten the future for the industry, we take one step back and look into a broader range of β-glucosidases that cross the life domains. β-Glucosidases from different subfamilies are compared systematically in their distribution, phylogenetic relationship, structure, in vivo function, mechanism of hydrolysis as well as substrate recognition, and kinetic profile. Protein engineering and application works on the enzymes are discussed based on the knowledge herein.
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Ouyang, H., Xu, F. (2016). The Role and Applications of β-Glucosidases in Biomass Degradation and Bioconversion. In: Gupta, V. (eds) Microbial Enzymes in Bioconversions of Biomass. Biofuel and Biorefinery Technologies, vol 3. Springer, Cham. https://doi.org/10.1007/978-3-319-43679-1_4
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