Skip to main content

Amino Acids, Indole-3-Acetic Acid, Stable and Transient Radicals, and Properties of Humic and Fulvic Acids as Affected by Tillage System

  • Chapter
  • First Online:
Book cover Bioactive Compounds in Agricultural Soils

Abstract

The concentration of bound amino acids and indole-3-acetic acid in humic acid (HA) and fulvic acid (FA) in samples collected from soils under no-tillage (NT) and conventional tillage (CT) management was measured. The samples were obtained from two long-term studies at the Horseshoe Bend (HSB) Experimental Area and at the Bledsoe Research Farm (BRF) in Georgia, USA. This study demonstrated the impact of NT and CT management on the content of amino acids in HA and FA. The total amount of bound amino acids in HA from NT was higher than in HA from CT. The contrary observation was noted for FA. Conventional tillage led to a decrease in the EPR signal intensity for HA, indicating a decrease in the dimension of the aromatic conjugation systems in these molecules. Transition from CT to NT is accompanied by a considerable rise in the signal intensity, which reflects more conjugation in the HA from NT compared to those from CT. Thus, HA from NT management from Horseshoe Bend Experimental Area and from Bledsoe Research Farm may be characterized by higher molecular weights and higher degree of condensation of aromatic constituents than HA from CT.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Alvarez CR, Alvarez R, Grigera MS, Lavado RS (1998) Associations between organic matter and fractions of the active microbial biomass. Soil Biol Biochem 30:767–773

    Article  CAS  Google Scholar 

  • Bambalov NN, Smychnik T, Maryganova V, Strigutsky V (2000) Peculiarities of the chemical composition and the molecular structure of peat humic substances. Acta Agroph 26:149–177

    Google Scholar 

  • Bayer C, Neto LM, Mielniczuk J, Ceretta CA (2000) Effect of no-till cropping systems on soil organic matter in sandy clay loam Acrisol from Southern Brazil monitored by electron spin resonance and nuclear magnetic resonance. Soil Till Res 53(2):95–104

    Article  Google Scholar 

  • Beare MH, Parmelee RW, Hendrix PF, Chang W, Coleman DC, Crossley DA Jr (1992) Microbial and faunal interactions and effects on litter nitrogen and decomposition agroecosystems. Ecol Mon 62:569–591

    Article  Google Scholar 

  • Blevins RL, Thomas GW, Cornelius PL (1977) Influence of no-tillage and nitrogen fertilization on certain soil properties after 5 year of continuous corn. Agron J 69:383–396

    Article  CAS  Google Scholar 

  • Blevins RL, Smith MS, Thomas GW (1984) Changes in soil properties under no-tillage. In: Phillips RE, Phillips SH (eds) No-tillage agriculture: principles and practices. Van Nostrad Reinhold, New York, pp 190–230

    Chapter  Google Scholar 

  • Bremner JM (1967) Nitrogenous compounds. In: McLaren AD, Peterson GH (eds) Soil biochemistry, vol 1. Marcel Dekker, New York, pp 19–66

    Google Scholar 

  • Carter MR, Rennie DA (1984) Dynamics of soil microbial biomass N under zero and shallow tillage for spring wheat, using 15 urea. Plant Soil 76:157–164

    Article  CAS  Google Scholar 

  • Chen Y, Senesi N, Schnitzer M (1977) Information provided on humic substances by E4/E6 ratios. Soil Sci Soc Am J 41:352–358

    Article  CAS  Google Scholar 

  • Claudius G, Merhotka R (1973) Root exudates from lentil/Lens culinaris Medik/seedlings in relation to wilt disease. Plant Soil 38:315–320

    Article  CAS  Google Scholar 

  • Czechowski F, Jezierski A (1997) EPR studies on petrographic constituents of bituminous coals, chars of brown coals group components, and humic acids 600°C char upon oxygen and solvent action. Energy Fuels 11:951–958

    Article  CAS  Google Scholar 

  • Dashman T, Stotzky G (1986) Microbial utilization of amino acids and peptide bound on homoionic montmorillonite and kaolinite. Soil Biol Biochem 18(1):5–14

    Article  CAS  Google Scholar 

  • Doran JW (1980) Soil microbial and biochemical changes associated with reduced tillage. Soil Sci Soc Am J 44:765–771

    Article  CAS  Google Scholar 

  • Drozd J, Jezierski A, Chen Y (1997) Chemical and ESR properties of municipal solid waste compost. In: Rosen D (ed) Modern agriculture and environment. Kluwer Academic Publishers, Dordrecht, pp 395–400

    Chapter  Google Scholar 

  • Durska G, Kaszubiak H (1980a) Occurrence of α, ε-diaminopimelic acid in soil. I. The content of α, ε-diaminopimelic acid in different soils. Pol ecol Stud 6(2):189–193

    CAS  Google Scholar 

  • Durska G, Kaszubiak H (1980b) Occurrence of α, ε-diaminopimelic acid in soil. II. Usefulness of α, ε-diaminopimelic acid determination for calculations of microbial biomass. Pol ecol Stud 6(2):195–199

    CAS  Google Scholar 

  • Durska G, Kaszubiak H (1980c) Occurrence of α, ε-diaminopimelic acid in soil. III. α, ε-diaminopimelic acid as the nutritional component of soil microorganisms. Pol ecol Stud 6(2):201–206

    CAS  Google Scholar 

  • Flaig W (1971) Organic compounds in soil. Soil Sci 111:19–33

    Article  CAS  Google Scholar 

  • Franzluebbers AJ (2004) Tillage and residue management effects on soil organic matter. In: Magdoff R, Weil RR (eds) Soil organic matter in sustainable agriculture. CRC Press, Boca Raton, pp 227–268

    Google Scholar 

  • Goh KM, Edmeades DC (1979) Distribution and partial characterisation of acid hydrolysable organic nitrogen in six New Zealand soils. Soil Biol Bioch 11:127–132

    Article  CAS  Google Scholar 

  • Gregorich EG, Carter MR, Angers AD, Monreal CM, Ellert BH (1994) Towards a minimum data set to assess soil organic matter quality in agricultural soils. Can J Soil Sci 74:367–385

    Article  CAS  Google Scholar 

  • Hargrove WL, Boswell FC, Langdale GW (eds) (1985) The rising hope of our land. Proceedings, Southern Region No-Till conference. July 16–17, 1985. Griffin, University of Georgia, Athens, USA. p 147

    Google Scholar 

  • Haworth RD (1971) The chemical nature of humic acid. Soil Sci 106(3):188–192

    Google Scholar 

  • Hendrix PF, Parmelee RW, Crossley DA Jr, Coleman DC, Odum EP, Groffman PM (1986) Detritus food webs in conventional and no-tillage agroecosystems. Bioscience 36(6):374–380

    Article  Google Scholar 

  • Herbek J, Murdock JT, Blevins R (1984) Effect of planting dates of no-till and conventional corn on soils with restricted drainage. Agronomy Notes. University of Kentucky. Department of Agronomy. 17.3

    Google Scholar 

  • Holland EA, Coleman DC (1987) Litter placement on microbial and organic matter dynamics in an agroecosystems. Ecology 68:425–433

    Article  Google Scholar 

  • Holtzclaw K, Schaumberg GD, LeVesque-Madore CS, Sposito G, Heick JA, Johnston CT (1980) Analytical properties of the soluble, metal-complexing fractions in sludge-soil mixtures: V. Amino acids, hexosamines, and other carbohydrates in fulvic acid. Soil Sci Soc Am J 44:736–740

    Article  CAS  Google Scholar 

  • House GJ, Stinner BR, Crossley DA Jr, Odum EP (1984) Nitrogen cycling in conventional and no-tillage agro-ecosystems: analysis of pathways and processes. J App Ecol 21:991–1012

    Article  Google Scholar 

  • Jerzykiewicz M, Drozd J, Jezierski A (1999) Organic radicals and paramagnetic metal complexes in municipal solid waste composts. An EPR and chemical study. Chemosphere 39:252–268

    Article  Google Scholar 

  • Jerzykiewicz M, Jezierski A, Czechowski F, Drozd J (2002) Influence of metal ions binding on free radical concentration in humic acids. A quantitative electron paramagnetic resonance study. Org Geochem 33:265–268

    Article  CAS  Google Scholar 

  • Jezierski A, Drozd J, Jerzykiewicz M, Chen Y, Kaye KJ (1998) EPR in the environmental control: copper complexes and free radicals in soil and municipal solid waste compost. Appl Magn Res 14:275–282

    Article  Google Scholar 

  • Jezierski A, Czechowski F, Jerzykiewicz M, Golonka I, Drozd J, Bylińska E, Chen Y, Seaward MRD (2002) Quantitative EPR study on free radicals in the natural polyphenols interacting with metal ions and other environmental pollutants. Spectrochim Acta 58:1293–1300

    Article  Google Scholar 

  • Juo ASR, Lal R (1979) Nutrient profile in a tropical Alfisol under conventional and no-till system. Soil Sci 127:168–173

    Article  CAS  Google Scholar 

  • Kalembasa S, Niewiński S (1990) The occurrence of amino acids in humic and fulvic acids extracted from arable and forest-grey-brown podzolic soils. Pol J Soil Sci 23:31–36

    CAS  Google Scholar 

  • Kofoed D, Nemming O, Brunfeldt K, Nebelin E, Thomsen J (1981) Investigations on the occurrence of nitrosamines in some agricultural products. Acta Agric Scand 31:40–48

    Article  CAS  Google Scholar 

  • Kögel-Knabner I (2002) The macromolecular organic composition of plant and microbial residues as inputs to soil organic matter. Soil Biol Biochem 34:139–162

    Article  Google Scholar 

  • Kuzyakov YV (1997) The role of amino acids and nucleic bases in turnover of nitrogen and carbon in soils humid fractions. Eur J Soil Sci 48:121–130

    Article  CAS  Google Scholar 

  • Larsson BK, sterdahl BG, Regner S (1990) Polycyclic aromatic hydrocarbons and volatile N-nitrosamines in some dried agricultural products. Swed J Agric Res 20:49–56

    CAS  Google Scholar 

  • Ley RE, Schmidt SK (2002) Fungal and bacterial responses to phenolic compounds and amino acids in high altitude barren soils. Soil Biol Biochem 34:989–995

    Article  CAS  Google Scholar 

  • Lipiec J, Stępniewski W (1995) Effects of soil compaction and tillage systems on uptake and losses of nutrients. Soil Till Res 35:37–52

    Article  Google Scholar 

  • Liu S-Y, Freyer AJ, Minard RD, Bollag J-M (1985) Enzyme-catalyzed complex-formation of amino acid esters and phenolic humus constituents. Soil Sci Soc Am J 49:337–342

    Article  CAS  Google Scholar 

  • Lowe LE (1973) Amino acids distribution in forest humus layers in British Columbia. Soil Sci Soc Am J 37:569–572

    Article  CAS  Google Scholar 

  • Malhotra SS, Sarkar SK (1979) Effects of sulphur dioxide on sugar and free amino acid content of pine seedlings. Plant Phys 47:223–227

    Article  CAS  Google Scholar 

  • Myhr K, Solberg Y, Selmer-Olsen AR (1978) The content of minerals, fibre, protein and amino acids in reed canary grass, timothy and meadow fescue. Acta Agric Scand 28(3):269–278

    Article  CAS  Google Scholar 

  • NeSmith DS, Hargrowe WL, Radcliffe DE, Tollner EW (1985) Tillage and residue management effects on soil physical properties. In: Hargrove WL, Boswell FC, Langdale GW (eds) The rising hope of our land. The rising hope of our land. Proceedings, Southern Region No-Till conference, July 16–17, 1985. Griffin, University of Georgia, Athens, USA. pp 87–92

    Google Scholar 

  • Orlov DS (1983) Humus acids of soils. Balkema A.A, Rotterdam, p 378

    Google Scholar 

  • Parmelee R, Beare MH, Blair JM (1989) Decomposition and nitrogen dynamics of surface weed residues in no-tillage agroecosystems under drought condition: influence of resources quality on the decomposer community. Soil Biol Biochem 21(1):97–103

    Article  Google Scholar 

  • Phillips RE, Phillips SH (1984) No-tillage agriculture: principles and practices. Van Nostrand Reinhold, New York, pp 171–189

    Book  Google Scholar 

  • Plaa GL (1980) Toxic responses of the liver. Toxicology. The basic sciences of poisons. Macmillan, New York, pp 216–231

    Google Scholar 

  • Ryszkowski L, Szajdak L, Karg J (1998) Effects of continuous cropping of rye on soil biota and biochemistry. Crit Rev Plant Sci 17(2):225–244

    Article  CAS  Google Scholar 

  • Schnitzer M, Sowden FJ, Ivarson KC (1974) Humic acid reactions with amino acids. Soil Biol Bioch 6:401–407

    Article  CAS  Google Scholar 

  • Scott DT, McKnight DM, Blunt-Harris EL, Kolesar SE, Lovley DR (1998) Quinone moieties act as electron acceptors in the reduction of humic substances by humic-reducing microorganisms. Environ Sci Technol 32:2984–2989

    Article  CAS  Google Scholar 

  • Seiter S, Horwath WR (2004) Strategic for managing soil organic matter to supply plant nutrients. In: Magdoff R, Weil RR (eds) Soil organic matter in sustainable agriculture. CRC Press, Boca Raton, pp 269–293

    Google Scholar 

  • Senesi N (1990) Molecular and quantitative aspects of the chemistry of fulvic acid and its interactions with metal ions and organic chemicals. Part I. The electron spin resonance approach. Anal Chim Acta 232:51–75

    Article  CAS  Google Scholar 

  • Senesi N (1992) Application of electron spin resonance and fluorescence spectroscopies to the study of humic substances. In: Kubat J (ed) Humus its structure and role in agriculture and environment. Elsevier Science B.V, Amsterdam, pp 11–26

    Chapter  Google Scholar 

  • Shu-Yen L, Freyer AJ, Minard RD, Bella JM (1985) Enzyme-catalyzed complex-formation of amino acids esters and phenolic humus constituents. Soil Sci Soc Am J 49:337–340

    Article  Google Scholar 

  • Smith WH (1976) Character and significance of forest tree root exudates. Ecology 57:324–331

    Article  CAS  Google Scholar 

  • Sörensen JH (1967) Duration of amino acid metabolites formed in soil during decomposition of carbohydrates. Soil Sci 104:204–241

    Article  Google Scholar 

  • Stearman GK, Lewis RJ, Tortorelli LJ, Tyler DD (1989a) Characterization of humic acids from no-tilled and tilled soils using carbon-13 nuclear magnetic resonance. Soil Sci Soc Am J 53:744–749

    Article  CAS  Google Scholar 

  • Stearman GK, Lewis RJ, Tortorelli LJ, Tyler DD (1989b) Herbicide reactivity of soil organic matter fractions in no-tilled and tilled cotton. Soil Sci Soc Am J 53:1690–1694

    Article  CAS  Google Scholar 

  • Stevenson G (1972) Biology of fungus, microbes and viruses. PWRiL, Warszawa, p 280 (in Polish)

    Google Scholar 

  • Stevenson FJ (1982a) Amino acids. In: Humus chemistry, genesis, composition, reactions. Wiley, New York, pp 65–78

    Google Scholar 

  • Stevenson FJ (1982b) Amino acids. In: Humus chemistry, genesis, composition, reactions. Wiley, New York, pp 264–284

    Google Scholar 

  • Stevenson FJ (1986) Cycle of soil: carbon, nitrogen, phosphorus, sulfur and micronutrients. Wiley, New York, pp 150–230

    Google Scholar 

  • Stinner BR, Crossley DA Jr, Odum EP, Todd RL (1984) Nutrient budgets and internal cycling of N, P, K, Ca, and Mg in conventional-tillage, no-tillage, and old-field ecosystems on the Georgia piedmont. Ecology 65:354–369

    Article  CAS  Google Scholar 

  • Strigutski VP, Novosha YuYu, Smychnik TP, Bambalov NN (1982) Investigation of the humic acids by non-linear EPR-spectroscopy. Sov Soil Sci (Pochvovedenie, in Russian) 1:14–151

    Google Scholar 

  • Swift RS (1996) Organic matter characterization. In: Methods of soil analysis. Part 3. Chemical methods-SSSA Book series no. 5. Madison. pp 1011–1069

    Google Scholar 

  • Szajdak L (1996) Impact of crop rotation and phenological periods on rhodanese activity and free sulfuric amino acids concentrations in soils under continuous rye cropping and crop rotation. Environ Int 22(5):563–569

    Article  CAS  Google Scholar 

  • Szajdak L, Österberg R (1996) Amino acids present in humic acids from soils under different cultivations. Environ Int 22(3):331–334

    Article  CAS  Google Scholar 

  • Szajdak L, Sokolov G (1997) Impact of different fertilizers on the bound amino acids content in soils. Int Peat J 7:29–32

    CAS  Google Scholar 

  • Szajdak L, Matuszewska T, Gawlik J (1998) Effect of secondary transformation state of peat-muck soils on their amino acids content. Int Peat J 8:76–80

    CAS  Google Scholar 

  • Szajdak L, Jezierski A, Cabrera ML (2003) Impact of conventional and no-tillage management on soils amino acids, stable and transient radicals and properties of humic and fulvic acids. Org Geochem 34:693–700

    Article  CAS  Google Scholar 

  • Tena M, Pinilla JA, Magallanes M (1986) L-phenylalanine deaminating activity in soil. Soil Biol Biochem 18(3):321–325

    Article  CAS  Google Scholar 

  • Tyler DD, Overton JR, Chambers AY (1983) Tillage effects on soil properties, diseases, cyst nematodes, and soybean yields. J Soil Water Conserv 38:374–376

    Google Scholar 

  • Van Doren DM, Triplett GB, Henry JE (1976) Influence of long-term tillage, crop rotation, and soil type combinations on corn yield. Soil Sci Soc Am J 40:100–105

    Article  Google Scholar 

  • Vancura V (1967) Root exudates of plant. III. Effects of temperature and “cold shock” on the exudation of various compounds from seeds and seedlings of maize and cucumber. Plant Soil 27:319–328

    Article  CAS  Google Scholar 

  • Von Hofe E, Schmerold I, Lijiński W, Jeltsch W, Kleihus P (1987) DNA methylation in rat tissues by series of homologous aliphatic nitrosamines ranging from N-nitrosodimethylamine to N-nitrosomethyldodecylamine. Carcinogenesis 8(9):1337–1341

    Article  Google Scholar 

  • Wells KL, Touchton JT (1985) Soil management and fertility for no-till production. In: Hargrove WL, Boswell FC, Langdale GW (eds) The rising hope of our land. Proceedings, Southern Region No-Till conference, July 16–17, 1985. Griffin, University of Georgia, Athens, USA. pp 71–77

    Google Scholar 

  • Worsham AD, Lewis WM (1985) Weed management: key to no-tillage crop production. Tillage and cropping sequence effects on yields and nitrogen use sufficiency. In: Hargrove WL, Boswell FC, Langdale GW (eds) The rising hope of our land. Proceedings, Southern Region No-Till conference, July 16–17, 1985. Griffin, University of Georgia, Athens, USA. pp 177–188

    Google Scholar 

  • Życzyńska-Bałoniak I, Szajdak L (1993) The content of bound amino acids in soil under rye monoculture and Norfolk crop rotation in different periods of plants development. Pol J Soil Sci 26(2):111–117

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Lech Wojciech Szajdak .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Szajdak, L.W., Cabrera, M.L., Jezierski, A. (2016). Amino Acids, Indole-3-Acetic Acid, Stable and Transient Radicals, and Properties of Humic and Fulvic Acids as Affected by Tillage System. In: Szajdak, L. (eds) Bioactive Compounds in Agricultural Soils. Springer, Cham. https://doi.org/10.1007/978-3-319-43107-9_5

Download citation

Publish with us

Policies and ethics