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Tissue Engineering of a Healthy Corneal Endothelium for FECD Patients

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Current Treatment Options for Fuchs Endothelial Dystrophy

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Abstract

The growing global shortage of corneal tissue for transplantation, in part exacerbated by population aging, has prompted the search for alternatives to native human corneas. Tissue engineering of a corneal endothelium appears to be a quite interesting option. The attractiveness of this approach resides in the possibility of expanding the number of cells obtained from a single donor, as one pair of donor corneas could theoretically treat hundreds of eyes. This chapter describes the key steps of corneal endothelial tissue engineering. It explains the importance of cell types, cell isolation, and expansion techniques, as well as cell quality assessment. It reviews the transplantation modalities that can be used to replace a diseased endothelium with a tissue-engineered endothelium, using or not a carrier. This chapter also covers the recent progress in corneal endothelial tissue engineering for Fuchs endothelial corneal dystrophy (FECD). Finally, it underlines the importance of new FECD models to advance our understanding of FECD pathophysiology and to develop or test new therapeutic options in order to cure or delay the progression of this disease.

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Authors and Affiliations

  1. Centre de recherche de l’Hôpital Maisonneuve-Rosemont, Montréal, QC, Canada

    Isabelle Brunette

  2. Département d’ophtalmologie, Université de Montréal, Montréal, QC, Canada

    Isabelle Brunette

  3. Centre de recherche du Centre hospitalier universitaire (CHU) de Québec – Université Laval, axe médecine régénératrice, Hôpital du Saint-Sacrement, Québec, QC, Canada

    Stéphanie Proulx

  4. Département d’Ophtalmologie, Université Laval, Québec, QC, Canada

    Stéphanie Proulx

  5. Centre de recherche en organogénèse expérimentale de l’Université Laval/LOEX, Québec, QC, Canada

    Stéphanie Proulx

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  1. Isabelle Brunette
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Correspondence to Isabelle Brunette .

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Editors and Affiliations

  1. Department of Ophthalmology, University of Cologne, Cologne, Germany

    Claus Cursiefen

  2. Wilmer Eye Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA

    Albert S. Jun

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Brunette, I., Proulx, S. (2017). Tissue Engineering of a Healthy Corneal Endothelium for FECD Patients. In: Cursiefen, C., Jun, A. (eds) Current Treatment Options for Fuchs Endothelial Dystrophy. Springer, Cham. https://doi.org/10.1007/978-3-319-43021-8_16

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