Abstract
Essentially, allergen components offer three possibilities to improve in vitro IgE diagnostics: (a) allergen components can be used individually for IgE determination, (b) allergen components can be combined as a mix in one test, and (c) individual allergen components can be specifically added to the extract. Option (a) is currently being used most extensively in practice, while (b) represents more of a theoretical possibility. The specific addition (“spiking”) of allergen components to an allergy extract (c) has been performed in the past for the ImmunoCAP® tests for latex (09/2001), hazelnut (05/2006), and yellow jacket venom (06/2012). Through this approach, underrepresented allergen components are supplemented, and the analytical sensitivity of the test systems can be significantly increased. In combination with molecular singleplex tests, these modified tests allow for new diagnostic possibilities. Clear communication from the manufacturer regarding in which test and from what time point on recombinant allergens were added – and where this was not done despite underrepresented allergen components – is important for the interpretation of the test results in routine clinical practice.
This contribution is based on the publication of the authors published 2015 in Allergo Journal International (Huss-Marp J, Raulf M, Jakob T: Spiking with recombinant allergens to improve allergen extracts: benefits and limitations for the use in routine diagnostics. Allergo J Int 2015;24:236–243 DOI 10.1007/s40629-015-0058-0) which has now been modified as book chapter.
The authors gratefully thank Prof. Robert G. Hamilton, PhD, Johns Hopkins Dermatology, Allergy and Clinical Immunology (DACI) Reference Laboratory at the Johns Hopkins Asthma & Allergy Center, Baltimore, MD, USA, for reviewing the manuscript, expert editorial assistance, and helpful suggestions regarding this chapter.
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Huss-Marp, J., Raulf, M., Jakob, T. (2017). Spiking with Recombinant Individual Allergens for Improvement of Allergen Extracts. In: Kleine-Tebbe, J., Jakob, T. (eds) Molecular Allergy Diagnostics. Springer, Cham. https://doi.org/10.1007/978-3-319-42499-6_8
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DOI: https://doi.org/10.1007/978-3-319-42499-6_8
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