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Testicular Sperm Retrieval for Cryopreservation in Cancer Patients

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The Complete Guide to Male Fertility Preservation

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Abstract

Young men undergoing cancer therapy, who might be interested in future fertility, should have sperm cryopreserved prior to start of therapy. Ideally, it is the ejaculated semen which is cryopreserved. However, in some situations this is not possible and testicular sperm need to be retrieved and cryopreserved. Testicular sperm retrieval may be achieved by a simple needle aspiration biopsy or may need an aggressive procedure like testicular microdissection. This chapter discusses in details the indications and techniques for testicular sperm retrieval for cryopreservation in men undergoing cancer therapy.

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References

  1. Bleyer A, Viny A, Barr R. Cancer epidemiology in older adolescents and young adults 15 to 29 years of age, including SEER incidence and survival: 1975–2000. National Institutes of Health Pub. No. 06-5767. Bethesda, MD: National Cancer Institute; 2006.

    Google Scholar 

  2. Schover LR, Brey K, Lichtin A, Lipshultz LI, Jeha S. Knowledge and experience regarding cancer, infertility, and sperm banking in younger male survivors. J Clin Oncol. 2002;20:1880–9.

    Article  PubMed  Google Scholar 

  3. Van Casteren NJ, Boellaard WP, Romijn JC, Dohle GR. Gonadal dysfunction in male cancer patients before cytotoxic treatment. Int J Androl. 2009;33:73–9.

    Article  PubMed  Google Scholar 

  4. Hendry WF, Stedronska J, Jones CR, Blackmore CA, Barrett A, Peckham MJ, et al. Semen analysis in testicular cancer and Hodgkin’s disease: pre- and post-treatment findings and implications for cryopreservation. Br J Urol. 1983;55(6):769–73.

    Article  CAS  PubMed  Google Scholar 

  5. Dieckmann KP, Linke J, Pichlmeier U, Kulejewski M, Loy V. Spermatogenesis in the contralateral testis of patients with testicular germ cell cancer: histological evaluation of testicular biopsies and comparison with healthy males. BJU Int. 2007;99(5):1079–85.

    Article  PubMed  Google Scholar 

  6. Jacobsen R, Bostofte E, Engholm G, Hansen J, Olsen JH, Skakkebaek NE, et al. Risk of testicular cancer in men with abnormal semen characteristics: cohort study. BMJ. 2000;321:789–92.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Devroey P, Liu J, Nagy Z, Goossens A, Tournaye H, Camus M, et al. Pregnancies after testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermia. Hum Reprod. 1995;10:1457–60.

    Article  CAS  PubMed  Google Scholar 

  8. Friedler S, Raziel A, Strassburger D, Soffer Y, Komarovsky D, Ron-El R. Testicular sperm retrieval by percutaneous fine needle sperm aspiration compared with testicular sperm extraction by open biopsy in men with non-obstructive azoospermia. Hum Reprod. 1997;12:1488–93.

    Article  CAS  PubMed  Google Scholar 

  9. Ramasamy R, Schlegel PN. Microdissection testicular sperm extraction: effect of prior biopsy on success of sperm retrieval. J Urol. 2007;177:1447–9.

    Article  PubMed  Google Scholar 

  10. Shah R. Surgical sperm retrieval: techniques and their indications. Indian J Urol. 2011;27(1):102–9.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Schrader M, Müller M, Sofikitis N, Straub B, Krause H, Miller K. “Onco-tese”: testicular sperm extraction in azoospermic cancer patients before chemotherapy-new guidelines? Urology. 2003;61(2):421–5.

    Article  CAS  PubMed  Google Scholar 

  12. Furuhashi K, Ishikawa T, Hashimoto H, Yamada S, Ogata S, Mizusawa Y, et al. Onco-testicular sperm extraction: testicular sperm extraction in azoospermic and very severely oligozoospermic cancer patients. Andrologia. 2013;45:107–10.

    Article  CAS  PubMed  Google Scholar 

  13. Hermann BP, Sukhwani M, Winkler F, Pascarella JN, Peters KA, Sheng Y, et al. Spermatogonial stem cell transplantation into rhesus testes regenerates spermatogenesis producing functional sperm. Cell Stem Cell. 2012;11:715–26.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Ginsberg JP, Carlson CA, Lin K, Hobbie WL, Wigo E, Wu X, et al. An experimental protocol for fertility preservation in prepubertal boys recently diagnosed with cancer: a report of acceptability and safety. Hum Reprod. 2010;25(1):37–41.

    Article  CAS  PubMed  Google Scholar 

  15. Shah RS. Surgical and non-surgical methods of sperm retrieval. In: Hansotia M, Desai S, Parihar M, editors. Advanced infertility management. New Delhi: Jaypee Brothers; 2002. p. 253–8.

    Google Scholar 

  16. Shah R, Nemade H. Is there an Ideal Sperm Retrieval Technique in ART? In: Arora S, Merchant R, Allahabadia G, editors. Reproductive medicine: challenges, solutions and breakthroughs. New Delhi: Jaypee Brothers; 2013. p 508–12

    Google Scholar 

  17. Smit M, Van Casteren NJ, Wildhagen MF, Romijn JC, Dohle GR. Sperm DNA integrity in cancer patients before and after cytotoxic treatment. Hum Reprod. 2010;25:1877–83.

    Article  CAS  PubMed  Google Scholar 

  18. Petersen PM, Skakkebaek NE, Rørth M, Giwercman A. Semen quality and reproductive hormones before and after orchiectomy in men with testicular cancer. J Urol. 1999;161(3):822–6.

    Article  CAS  PubMed  Google Scholar 

  19. Schlegel PN, Su LM. Physiological consequences of testicular sperm extraction. Hum Reprod. 1997;12:1688–92.

    Article  CAS  PubMed  Google Scholar 

  20. Ramasamy R, Yagan N, Schlegel PN. Structural and functional changes to the testis after conventional versus microdissection testicular sperm extraction. Urology. 2005;65:1190–4.

    Article  PubMed  Google Scholar 

  21. Schill T, Bals-Pratsch M, Küpker W, Sandmann J, Johannisson R, Diedrich K. Clinical and endocrine follow-up of patients after testicular sperm extraction. Fertil Steril. 2003;79:281–6.

    Article  PubMed  Google Scholar 

  22. Prins GS, Dolgina R, Studney P, Kaplan B, Ross L, Niederberger C. Quality of cryopreserved testicular sperm in patients with obstructive and nonobstructive azoospermia. J Urol. 1999 May;161(5):1504–8.

    Article  CAS  PubMed  Google Scholar 

  23. Hessel M, Robben JC, D'Hauwers KW, Braat DD, Ramos L. The influence of sperm motility and cryopreservation on the treatment outcome after intracytoplasmic sperm injection following testicular sperm extraction. Acta Obstet Gynecol Scand. 2015 Dec;94(12):1313–21.

    Article  PubMed  Google Scholar 

  24. Stein A, Shufaro Y, Hadar S, Fisch B, Pinkas H. Successful use of the Cryolock device for cryopreservation of scarce human ejaculate and testicular spermatozoa. Andrology. 2015;3(2):220–4.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Division Urooncology, Department of Surgical Oncology, Tata Memorial Hospital, Mumbai, Maharashtra, India

    Gagan Prakash MS, DNB

  2. Department of Urology-Andrology, Lilavati Hospital & Research Centre, Bandra Reclamation, Mumbai, 400050, Maharashtra, India

    Rupin Shah MS, MCh

Authors
  1. Gagan Prakash MS, DNB
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  2. Rupin Shah MS, MCh
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Corresponding author

Correspondence to Rupin Shah MS, MCh .

Editor information

Editors and Affiliations

  1. Department of Urology, Hamad Medical Corporation, Doha, Qatar

    Ahmad Majzoub

  2. Department of Urology, Andrology Center, Cleveland Clinic, Cleveland, Ohio, USA

    Ashok Agarwal

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Prakash, G., Shah, R. (2018). Testicular Sperm Retrieval for Cryopreservation in Cancer Patients. In: Majzoub, A., Agarwal, A. (eds) The Complete Guide to Male Fertility Preservation. Springer, Cham. https://doi.org/10.1007/978-3-319-42396-8_3

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  • DOI: https://doi.org/10.1007/978-3-319-42396-8_3

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-42395-1

  • Online ISBN: 978-3-319-42396-8

  • eBook Packages: MedicineMedicine (R0)

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