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The Process of Sperm Cryopreservation, Thawing and Washing Techniques

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Abstract

Sperm cryopreservation utilizes state-of-the-art technology for fertility preservation in men who have diverse conditions that require freezing sperm for future use in ART. Sperm cryopreservation is of significance in order for these men with disease conditions to have a good quality of life. In this chapter we discuss the different indications for cryopreservation of sperm in men with cancer, infertility issues, extreme medical conditions and even posthumous sperm retrieval. The two major groups for sperm freezing, the auto-conservation group and the donor group, are discussed. The various standard of care and experimental techniques as well as protocols for sperm freezing and the advantages and disadvantages of each technique are described in the chapter. The chapter also provides a description of the current challenges and future research directions for sperm cryopreservation.

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Correspondence to Ashok Agarwal PhD .

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Gupta, S., Sharma, R., Agarwal, A. (2018). The Process of Sperm Cryopreservation, Thawing and Washing Techniques. In: Majzoub, A., Agarwal, A. (eds) The Complete Guide to Male Fertility Preservation. Springer, Cham. https://doi.org/10.1007/978-3-319-42396-8_14

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