Biophysical Methods of Detection and Quantification of Uptake, Translocation, and Accumulation of Nanoparticles

  • Illya A. Medina-Velo
  • Nubia Zuverza-Mena
  • Wenjuan Tan
  • Jose A. Hernandez-Viezcas
  • Jose R. Peralta-Videa
  • Jorge L. Gardea-TorresdeyEmail author


Manufactured nanomaterials (MNMs) are more frequently found in consumer products as well as in industrial and agricultural applications. The high volume of production, use, and disposal of MNM-containing wastes increase the probability of release of these products to the environment. An ever-increasing number of articles have shown that MNMs impact plants and other organisms in different ways. In this chapter, we discuss the biophysical methods currently used to measure the uptake, translocation, accumulation, and speciation of MNMs within plants. We included methods used to analyze plants exposed to carbon-based and metal-based MNMs. Advantages and disadvantages of each analytical technique are discussed.


Nanoparticles Plants Absorption Detection Quantification Microscopy Spectroscopy 



This material is based upon work supported by the National Science Foundation and the Environmental Protection Agency under Cooperative Agreement Number DBI-0830117. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation or the Environmental Protection Agency. This work has not been subjected to EPA review, and no official endorsement should be inferred. This work was also supported by Grant 2G12MD007592 from the National Institutes on Minority Health and Health Disparities (NIMHD), a component of the National Institutes of Health (NIH). Authors also acknowledge the USDA grant number 2011-38422-30835 and the NSF Grants # CHE-0840525 and DBI 1429708. Partial funding was provided by the NSF ERC on Nanotechnology-Enable Water Treatment (EEC-1449500). J. L. Gardea-Torresdey acknowledges the Dudley family for the Endowed Research Professorship, the Academy of Applied Science/US Army Research Office, Research and Engineering Apprenticeship program (REAP) at UTEP, grant # W11NF-10-2-0076, sub-grant 13-7, and STARs programs of the University of Texas System. N. Zuverza-Mena and I.A. Medina-Velo thank the support of Consejo Nacional de Ciencia y Tecnologia of Mexico (CONACyT).


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Illya A. Medina-Velo
    • 1
    • 4
  • Nubia Zuverza-Mena
    • 3
    • 4
  • Wenjuan Tan
    • 1
    • 4
  • Jose A. Hernandez-Viezcas
    • 1
    • 4
  • Jose R. Peralta-Videa
    • 1
    • 2
    • 4
  • Jorge L. Gardea-Torresdey
    • 1
    • 2
    • 4
    Email author
  1. 1.Department of ChemistryThe University of Texas at El PasoEl PasoUSA
  2. 2.Environmental Science and Engineering Ph.D. ProgramThe University of Texas at El PasoEl PasoUSA
  3. 3.Metallurgical and Materials Engineering DepartmentThe University of Texas at El PasoEl PasoUSA
  4. 4.University of California Center for Environmental Implications of Nanotechnology (UC CEIN), The University of Texas at El PasoEl PasoUSA

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