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Identification, Quantification, and Site Localization of Protein Posttranslational Modifications via Mass Spectrometry-Based Proteomics

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Modern Proteomics – Sample Preparation, Analysis and Practical Applications

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 919))

Abstract

Posttranslational modifications (PTMs) are important biochemical processes for regulating various signaling pathways and determining specific cell fate. Mass spectrometry (MS)-based proteomics has been developed extensively in the past decade and is becoming the standard approach for systematic characterization of different PTMs on a global scale. In this chapter, we will explain the biological importance of various PTMs, summarize key innovations in PTMs enrichment strategies, high-performance liquid chromatography (HPLC)-based fractionation approaches, mass spectrometry detection methods, and lastly bioinformatic tools for PTMs related data analysis. With great effort in recent years by the proteomics community, highly efficient enriching methods and comprehensive resources have been developed. This chapter will specifically focus on five major types of PTMs; phosphorylation, glycosylation, ubiquitination/sumosylation, acetylation, and methylation.

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    Authors and Affiliations

    1. Department of Chemistry, South University of Science and Technology of China, 518055, Shenzhen, China

      Mi Ke, Hainan Shen, Linjue Wang, Shusheng Luo, Lin Lin, Jie Yang & Ruijun Tian

    2. Shenzhen Key Laboratory of Cell Microenvironment, South University of Science and Technology of China, 518055, Shenzhen, China

      Ruijun Tian

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    1. UT Southwestern Medical Center, Dallas, Texas, USA

      Hamid Mirzaei

    2. Biotech Division, Neurophagy Therapeutics, INC, Odessa, Texas, USA

      Martin Carrasco

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    Ke, M. et al. (2016). Identification, Quantification, and Site Localization of Protein Posttranslational Modifications via Mass Spectrometry-Based Proteomics. In: Mirzaei, H., Carrasco, M. (eds) Modern Proteomics – Sample Preparation, Analysis and Practical Applications. Advances in Experimental Medicine and Biology, vol 919. Springer, Cham. https://doi.org/10.1007/978-3-319-41448-5_17

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