Abstract
The objective of this research was to determine the effect of fiber architecture on the cell viability of the electrospun nanofiber (ENF) membrane. Three different ENF membranes (consists of single layer, four layers and six layers of cross-direction ENF layer) were developed and deposited on one side of the acrylic mold. The fibrous side of the mold was placed on the top of a well plate. Mouse osteoblast cell adhesion and proliferation on each of the different kinds of membranes were observed using a fluorescent microscope technique. Results shows that the cell adhered and proliferated on each kind of ENF fiber membranes. Tests shows that the designed ENF membrane has no adverse effect on osteoblast cells, fiber attached to the fiber, and strongly dependent on the fiber layer numbers in the membrane.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Deitzel, J., et al.: Controlled deposition of electrospun poly(ethylene oxide) fibers. Polymer 42, 8163–8170 (2001)
Bosworth, L.A., Turner, L.-A., Cartmell, S.H.: State of the art composites comprising electrospun fibres coupled with hydrogels: a review. Nanomed. Nanotechnol. Biol. Med. 9(3), 322–335 (2013)
Mizuno, H., et al.: Biomechanical and biochemical characterization of composite tissue-engineered intervertebral discs. Biomaterials 27(3), 362–370 (2006)
Khandaker, M., Snow, P.: Method and apparatus for the controlled deposition of branched electrospun fiber on biomedical implants and material (U.S. Patent Application No. 14/734147, International Patent Application No. PCT/US15/45183, and U.S. Patent 9,359,694)
Acknowledgements
This research was made possible by grant 5P20GM103447 from the US National Institutes of Health and an on-campus faculty grant program from the University of Central Oklahoma Office of Research and Grants.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 The Society for Experimental Mechanics, Inc.
About this paper
Cite this paper
Sultana, F., Vaughan, M., Khandaker, M. (2017). Effect of Fiber Architecture on the Cell Functions of Electrospun Fiber Membranes. In: Korach, C., Tekalur, S., Zavattieri, P. (eds) Mechanics of Biological Systems and Materials, Volume 6. Conference Proceedings of the Society for Experimental Mechanics Series. Springer, Cham. https://doi.org/10.1007/978-3-319-41351-8_22
Download citation
DOI: https://doi.org/10.1007/978-3-319-41351-8_22
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-41350-1
Online ISBN: 978-3-319-41351-8
eBook Packages: EngineeringEngineering (R0)