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Clinical Islet Isolation

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Pancreatic Islet Isolation

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 938))

Abstract

The overarching success of islet transplantation relies on the success in the laboratory to isolate the islets. This chapter focuses on the processes of human islet cell isolation and the ways to optimally provide islet cells for transplantation. The major improvements in regards to the choice of enzyme type, way the digested pancreas tissue is handled to best separate islets from the acinar and surrounding tissues, the various methods of purification of the islets, their subsequent culture and quality assurance to improve outcomes to culminate in safe and effective islet transplantation will be discussed. After decades of improvements, islet cell isolation and transplantation now clearly offer a safe, effective and feasible therapeutic treatment option for an increasing number of patients suffering from type 1 diabetes specifically for those with severe hypoglycaemic unawareness.

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Abbreviations

BMI:

Body mass index

BSE:

Bovine spongiform encephalopathy

CMRL:

Connaught medical research laboratories

IEQ :

Islet cell isolation

IEQ /g:

Islet equivalent islet equivalents per gram

MTC:

Mixed treatment comparison

T1D :

Type 1 diabetes

UW:

University of Wisconsin solution

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Authors and Affiliations

  1. National Pancreas and Islet Transplant Laboratories, The Westmead Institute for Medical Research, Westmead, NSW, 2145, Australia

    Wayne J. Hawthorne, Lindy Williams & Yi Vee Chew

  2. Department of Surgery, Westmead Clinical School, Westmead Hospital, University of Sydney, Westmead, NSW, 2145, Australia

    Wayne J. Hawthorne

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  1. Laboratory of Cell Therapy of Diabetes, Department of Pediatrics, Faculty of Medicine and Odontology, Hospital Cruces, University of the Basque Country (UPV/EHU), Leioa, Spain

    Miriam Ramírez-Domínguez

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Hawthorne, W.J., Williams, L., Chew, Y.V. (2016). Clinical Islet Isolation. In: Ramírez-Domínguez, M. (eds) Pancreatic Islet Isolation. Advances in Experimental Medicine and Biology, vol 938. Springer, Cham. https://doi.org/10.1007/978-3-319-39824-2_7

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