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Pancreatic Islet Isolation pp 57–66Cite as

Isolation of Pancreatic Islets from Nonhuman Primates

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 938))

Abstract

Nonhuman primates (NHP) constitute a highly relevant pre-clinical animal model to develop strategies for beta cell replacement. The close phylogenetic and immunologic relationship between NHP and humans results in cross-reactivity of various biological agents with NHP cells, as well as a very similar cytoarchitecture between islets from human and NHP that is strikingly different from that observed in rodent islets. The composition and location of endocrine cells in human or NHP islets, randomly distributed and associated with blood vessels, have functional consequences and a predisposition for paracrine interactions. Furthermore, translation of approaches that proved successful in rodent models to the clinic has been limited. Consequently, data collected from NHP studies can form the basis for an IND submission to the FDA. This chapter describes in detail the key aspects for isolation of islets from NHP, from organ procurement up to assessment of islet function, comparing and emphasizing the similarities between isolation procedures for human and NHP islets.

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Acknowledgement

I want to thank Dr. Norma S. Kenyon, whose expert guidance and support enabled me to become proficient in the field of NHP islet isolation .

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Authors and Affiliations

  1. Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 1450 NW 10 Avenue, Miami, FL, USA

    Dora M. Berman

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  1. Dora M. Berman
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Correspondence to Dora M. Berman .

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Editors and Affiliations

  1. Laboratory of Cell Therapy of Diabetes, Department of Pediatrics, Faculty of Medicine and Odontology, Hospital Cruces, University of the Basque Country (UPV/EHU), Leioa, Spain

    Miriam Ramírez-Domínguez

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Berman, D.M. (2016). Isolation of Pancreatic Islets from Nonhuman Primates. In: Ramírez-Domínguez, M. (eds) Pancreatic Islet Isolation. Advances in Experimental Medicine and Biology, vol 938. Springer, Cham. https://doi.org/10.1007/978-3-319-39824-2_5

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