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Diagnostic Flow Cytometry and Immunophenotypic Classification

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Childhood Acute Lymphoblastic Leukemia

Abstract

Haemopoiesis begins with a quiescent stem cell that gives rise to daughter cells capable of differentiation along multiple lineages. Differentiation progresses in a series of stages to produce functional, mature cells of all lineages and is orchestrated by sequential gene expression [1]. CD antigens are cell surface proteins which have diverse functional roles in haemopoiesis including signal transduction, enzymes, growth factor receptors and adhesion molecules and can be widely expressed or restricted to a specific stage of maturation/activation of a defined lineage. Thus, patterns of CD antigen expression can identify the lineage, maturation and functional stages of cells and are invaluable for evaluating normal haemopoiesis and the malignant state, including ALL.

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Authors and Affiliations

  1. Wolfson Childhood Cancer Research Centre, Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK

    Julie Irving

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Correspondence to Julie Irving .

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Editors and Affiliations

  1. Department of Paediatric Haemotology, Sheffield Children’s Hospital NHS Trust, University of Sheffield, Sheffield, South Yorkshire, United Kingdom

    Ajay Vora

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Irving, J. (2017). Diagnostic Flow Cytometry and Immunophenotypic Classification. In: Vora, A. (eds) Childhood Acute Lymphoblastic Leukemia. Springer, Cham. https://doi.org/10.1007/978-3-319-39708-5_3

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  • DOI: https://doi.org/10.1007/978-3-319-39708-5_3

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-39707-8

  • Online ISBN: 978-3-319-39708-5

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