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Assembly, Engineering and Applications of Virus-Based Protein Nanoparticles

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Protein-based Engineered Nanostructures

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 940))

Abstract

Viruses and their protein capsids can be regarded as biologically evolved nanomachines able to perform multiple, complex biological functions through coordinated mechano-chemical actions during the infectious cycle. The advent of nanoscience and nanotechnology has opened up, in the last 10 years or so, a vast number of novel possibilities to exploit engineered viral capsids as protein-based nanoparticles for multiple biomedical, biotechnological or nanotechnological applications. This chapter attempts to provide a broad, updated overview on the self-assembly and engineering of virus capsids, and on applications of virus-based nanoparticles. Different sections provide outlines on: (i) the structure, functions and properties of virus capsids; (ii) general approaches for obtaining assembled virus particles; (iii) basic principles and events related to virus capsid self-assembly; (iv) genetic and chemical strategies for engineering virus particles; (v) some applications of engineered virus particles being developed; and (vi) some examples on the engineering of virus particles to modify their physical properties, in order to improve their suitability for different uses.

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Abbreviations

AAV:

adeno-associated viruses

AFM:

atomic force microscopy

BMV:

brome mosaic virus

CA:

capsid protein of HIV

CBB:

capsid building block

CCMV:

cowpea chlorotic mottle virus

CNT:

classic nucleation theory

CP:

capsid protein

CPMV:

cowpea mosaic virus

CMV:

cucumber mosaic virus

CPV:

canine parvovirus

cryo-EM:

cryo-electron microscopy

ds:

double-stranded

EMDB:

Electron Microscopy Database

FMDV:

foot-and-mouth disease virus

FRET:

Förster resonance energy transfer

HBV:

hepatitis B virus

HCRSV:

Hibiscus chlorotic ringspot virus

HIV:

human immunodeficiency virus

HPV:

human papillomavirus

HRV:

human rhinovirus

HSV-1:

herpes simplex virus type 1

MD:

molecular dynamics

MRI:

magnetic resonance imaging

MS:

mass spectrometry

MVM:

minute virus of mice

NP:

nanoparticle

PCR:

polymerase chain reaction

PDB:

Protein Data Bank

PEG:

polyethyleneglycol

RCNMV:

red clover nechrotic mottle virus

SBMV:

southern bean mosaic virus

ss:

single-stranded

STNV:

satellite tobacco necrosis virus

SV40:

simian virus 40

TBSV:

tomato bushy stunt virus

TMV:

tobacco mosaic virus

VLP:

virus-like particle

VP:

viral (capsid) protein.

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Acknowledgments

I gratefully acknowledge former and current collaborators and members of my group for their invaluable contributions to our studies on structure-properties-function relationships and engineering of virus particles, and Miguel Angel Fuertes for help with figures in this chapter. This work was funded by grants from MINECO/FEDER EU (BIO2012-37649 and BIO2015-69928-R) and by an institutional grant from Fundacion Ramon Areces. The author is an associate member of the Institute for Biocomputation and Physics of Complex Systems, Zaragoza, Spain.

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  1. Centro de Biología Molecular “Severo Ochoa” (CSIC-UAM), Universidad Autónoma de Madrid, Campus de Cantoblanco, 28049, Madrid, Spain

    Mauricio G. Mateu

  2. Department of Molecular Biology, Universidad Autónoma de Madrid, Campus de Cantoblanco, 28049, Madrid, Spain

    Mauricio G. Mateu

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Correspondence to Mauricio G. Mateu .

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  1. CIC BiomaGUNE, Donostia-San Sebastián, Spain

    Aitziber L. Cortajarena

  2. Department of Chemistry, Virginia Tech, Blacksburg, Virginia, USA

    Tijana Z. Grove

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Mateu, M.G. (2016). Assembly, Engineering and Applications of Virus-Based Protein Nanoparticles. In: Cortajarena, A., Grove, T. (eds) Protein-based Engineered Nanostructures. Advances in Experimental Medicine and Biology, vol 940. Springer, Cham. https://doi.org/10.1007/978-3-319-39196-0_5

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