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Designed Repeat Proteins as Building Blocks for Nanofabrication

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Protein-based Engineered Nanostructures

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 940))

Abstract

This chapter will focus on the description of protein-based nanostructures. How proteins can be used as molecular units in order to generate complex materials and structures? What are the key aspects to achieve defined final properties, including shape, stability, function, and order at different length scales by modifying the protein sequence at the modular level?

As described in other chapters of the book, we will review the basic concepts and the latest achievements in protein engineering toward nanotechnological applications. Particularly in this chapter the main focus will be on a particular type of proteins, repeat proteins. Because of their modular nature, these proteins are better suited to be used as building blocks than other protein scaffolds. First, we describe general concepts of the protein-based assemblies. Then we introduce repeat proteins and describe the properties that will impact their use in nanotechnology. In particular, we focus on a system based on a synthetic protein, the consensus tetratricopeptide repeat (CTPR). We review recent works from other groups and our group in which the potential of these repeat protein scaffolds is exploited for the fabrication of different protein assemblies, and as biomolecular templates to arrange different molecules and nanoscale objects.

*Author contributed equally with all other contributors.

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Acknowledgements

Financial support for this research was obtained from the Spanish Ministerio de Economía y Competitividad (BIO2012-34835), the European Commission International Reintegration Grant (IRG-246688), ERC-CoG-648071, and Marie Curie COFUND “AMAROUT-Europe” Programme (A.L.C.). S.H.M. thanks IMDEA-Nanociencia for financial support through an “Ayuda de Iniciación a la Investigación” fellowship and the Basque Government for financial support (PhD Scholarship).

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    Authors and Affiliations

    1. CIC BiomaGUNE, Paseo Miramón 182, Donostia-San Sebastián, 20009, Spain

      Sara H. Mejias, Antonio Aires, Pierre Couleaud & Aitziber L. Cortajarena

    2. IMDEA-Nanociencia, Campus de Cantoblanco, 28049, Madrid, Spain

      Antonio Aires & Aitziber L. Cortajarena

    3. Ikerbasque, Basque Foundation for Science, Mª Díaz de Haro 3, 48013, Bilbao, Spain

      Aitziber L. Cortajarena

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    1. Sara H. Mejias
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    Correspondence to Aitziber L. Cortajarena .

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    1. CIC BiomaGUNE, Donostia-San Sebastián, Spain

      Aitziber L. Cortajarena

    2. Department of Chemistry, Virginia Tech, Blacksburg, Virginia, USA

      Tijana Z. Grove

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    © 2016 Springer International Publishing Switzerland

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    Mejias, S.H., Aires, A., Couleaud, P., Cortajarena, A.L. (2016). Designed Repeat Proteins as Building Blocks for Nanofabrication. In: Cortajarena, A., Grove, T. (eds) Protein-based Engineered Nanostructures. Advances in Experimental Medicine and Biology, vol 940. Springer, Cham. https://doi.org/10.1007/978-3-319-39196-0_4

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