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Designed Protein Origami

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Protein-based Engineered Nanostructures

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 940))

Abstract

Proteins are highly perfected natural molecular machines, owing their properties to the complex tertiary structures with precise spatial positioning of different functional groups that have been honed through millennia of evolutionary selection. The prospects of designing new molecular machines and structural scaffolds beyond the limits of natural proteins make design of new protein folds a very attractive prospect. However, de novo design of new protein folds based on optimization of multiple cooperative interactions is very demanding. As a new alternative approach to design new protein folds unseen in nature, folds can be designed as a mathematical graph, by the self-assembly of interacting polypeptide modules within the single chain. Orthogonal coiled-coil dimers seem like an ideal building module due to their shape, adjustable length, and above all their designability. Similar to the approach of DNA nanotechnology, where complex tertiary structures are designed from complementary nucleotide segments, a polypeptide chain composed of a precisely specified sequence of coiled-coil forming segments can be designed to self-assemble into polyhedral scaffolds. This modular approach encompasses long-range interactions that define complex tertiary structures. We envision that by expansion of the toolkit of building blocks and design strategies of the folding pathways protein origami technology will be able to construct diverse molecular machines.

*Author contributed equally with all other contributors.

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    Authors and Affiliations

    1. Laboratory of Biotechnology, National Institute of Chemistry, Ljubljana, Slovenia

      Igor Drobnak, Ajasja Ljubetič, Helena Gradišar & Roman Jerala

    2. EN-FIST Centre of Excellence, Ljubljana, Slovenia

      Helena Gradišar & Roman Jerala

    3. Faculty of Mathematics and Physics, University of Ljubljana, Ljubljana, Slovenia

      Tomaž Pisanski

    4. University of Primorska, Koper, Slovenia

      Tomaž Pisanski

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    1. Igor Drobnak
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    Correspondence to Roman Jerala .

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    1. CIC BiomaGUNE, Donostia-San Sebastián, Spain

      Aitziber L. Cortajarena

    2. Department of Chemistry, Virginia Tech, Blacksburg, Virginia, USA

      Tijana Z. Grove

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    Drobnak, I., Ljubetič, A., Gradišar, H., Pisanski, T., Jerala, R. (2016). Designed Protein Origami. In: Cortajarena, A., Grove, T. (eds) Protein-based Engineered Nanostructures. Advances in Experimental Medicine and Biology, vol 940. Springer, Cham. https://doi.org/10.1007/978-3-319-39196-0_2

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