Skip to main content
SpringerLink
Log in

Temporal Change of Alcian Blue-Stained Primo Vascular System in Lymph Vessels of Rats

  • Conference paper
  • First Online:
Oxygen Transport to Tissue XXXVIII

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 923))

Abstract

This study aims to investigate the temporal change of a vascular system now known as the primo vascular system (PVS). We used Alcian blue (AB) dye for imaging the distribution of the PVS in lymphatic vessels. The target lymph vessels were chosen as they are easily accessible from the skin, and long-term observation is possible with intact physiological conditions due to a minimal surgical procedure. AB solution was injected into the inguinal lymph node and the target lymph vessels were located along the superficial epigastric vessels. The imaging system allowed processing for extraction of images showing changes in the AB intensity of the visualized PVS components. This newly developed procedure can be used for further study on various dynamic processes of PVS in lymph vessels.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Kim BH (1963) On the Kyungrak system. J Acad Med Sci DPRK 90:1–35

    Google Scholar 

  2. Kang KA (2013) Historical observations on the half-century freeze in research between the Bonghan system and the primo vascular system. J Acupunct Meridian Stud 6(6):285–292

    Article  PubMed  Google Scholar 

  3. Soh KS (2009) Bonghan circulatory system as an extension of acupuncture meridians. J Acupunct Meridian Stud 2(6):93–106

    Article  PubMed  Google Scholar 

  4. Lee BC, Yoo JS, Baik KY et al (2005) Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method. Anat Rec B New Anat 286B:1–7

    Article  Google Scholar 

  5. Lee C, Seol SK, Lee BC et al (2006) Alcian blue staining method to visualize Bonghan threads inside large caliber lymphatic vessels and X-ray microtomography to reveal their microchannels. Lymphat Res Biol 4:181–189. doi:10.1089/lrb.2006.4402

    Article  CAS  PubMed  Google Scholar 

  6. Johng HM, Yoo JS, Yoon TJ et al (2007) Use of magnetic nanoparticles to visualize threadlike structures inside lymphatic vessels of rats. eCAM 4(1):77–82. doi:10.1093/ecam/nel057

    PubMed  Google Scholar 

  7. Lee BC, Soh KS (2008) Contrast-enhancing optical method to observe a Bonghan duct floating inside a lymph vessel of a rabbit. Lymphology 41:178–185

    CAS  PubMed  Google Scholar 

  8. Ogay V, Bae KH, Kim KW, Soh KS (2009) Comparison of the characteristic features of Bonghan ducts, blood and lymphatic capillaries. J Acupunct Meridian Stud 2(2):107–117

    Article  PubMed  Google Scholar 

  9. Choi IH, Jeong HK, Hong YK (2011) Detection of the primo vessels in the rodent thoracic lymphatic ducts. In: Soh KS, Kang KA, Harrison D (eds) The primo vascular system: its role in cancer and regeneration. Springer, New York, pp 25–40

    Google Scholar 

  10. Lee SH, Bae KH, Kim GO et al (2013) Primo vascular system in the lymph vessel from the inguinal to the axillary nodes. Evid Based Complement Alternat Med 2013:472704, Article ID 472704. http://dx.doi.org/10.1155/2013/472704

  11. Jung SJ, Bae KH, Nam MH et al (2013) Primo vascular system floating in lymph ducts of rats. J Acupunct Meridian Stud 6(6):306–318

    Article  PubMed  Google Scholar 

  12. Kwon BS, Ha CM, Yu S, Lee BC, Ro JY, Hwang S (2012) Microscopic nodes and ducts inside lymphatics and on the surface of internal organs are rich in granulocytes and secretory granules. Cytokine 60:587–592

    Article  CAS  PubMed  Google Scholar 

  13. Lee SJ, Park SH, Kim YI et al (2014) Adult stem cells from the hyaluronic acid-rich node and duct system (HAR-NDS) differentiate into neuronal cells and repair brain injury. Stem Cells Dev 23(23):2831–2840. doi:10.1089/scd.2014.0142

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Kang KA (2015) Personal communication

    Google Scholar 

Download references

Acknowledgements

This work was supported in part by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (grant number: 2013R1A1A2008343).

Author information

Authors and Affiliations

  1. Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon, 443-270, South Korea

    Jungdae Kim, Dong-Hyun Kim, Sharon Jiyoon Jung & Kwang-Sup Soh

  2. Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, Suwon, 443-270, South Korea

    Sharon Jiyoon Jung

Authors
  1. Jungdae Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dong-Hyun Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sharon Jiyoon Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kwang-Sup Soh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kwang-Sup Soh .

Editor information

Editors and Affiliations

  1. Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China

    Qingming Luo

  2. Molecular Imaging Laboratory, Department of Radiology, Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA

    Lin Z. Li

  3. Microvascular Measurements, St. Lorenzen, Italy

    David K. Harrison

  4. Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China

    Hua Shi

  5. ISOTT Historian, Ellicott City, Maryland, USA

    Duane F. Bruley

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer International Publishing Switzerland

About this paper

Cite this paper

Kim, J., Kim, DH., Jung, S.J., Soh, KS. (2016). Temporal Change of Alcian Blue-Stained Primo Vascular System in Lymph Vessels of Rats. In: Luo, Q., Li, L., Harrison, D., Shi, H., Bruley, D. (eds) Oxygen Transport to Tissue XXXVIII. Advances in Experimental Medicine and Biology, vol 923. Springer, Cham. https://doi.org/10.1007/978-3-319-38810-6_41

Download citation

Publish with us

Policies and ethics

Search

Navigation