Skip to main content

Table 4.1 Protein secondary structure content of SbmA and BacA membrane proteins with and without addition of Bac7 ligand up to 1:35 molar ratios calculated from SRCD data using CONTINLL algorithm (Sreerama and Woody 2004) with SMP50 reference data set of 37 soluble proteins and 13 membrane proteins applied through CDApps beamline software (Hussain et al. 2015)

From: Characterisation of Conformational and Ligand Binding Properties of Membrane Proteins Using Synchrotron Radiation Circular Dichroism (SRCD)

Protein secondary structure elements (SSE) SbmA SbmA + Bac7 [1–35] BacA BacA + Bac7 [1–35]
H1, α-helix 0.41 0.32 0.41 0.46
H2, distorted α-helix 0.19 0.17 0.19 0.19
S1, β-strand 0.04 0.09 0.06 0.03
S2, distorted β-strand 0.04 0.04 0.03 0.02
T, turn 0.12 0.10 0.12 0.10
U, unordered 0.21 0.28 0.20 0.20
Spectral fit SD 0.06 0.04 0.06 0.04
  1. Redrawn from Runti et al. 2013