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Mitochondrial DNA Based Molecular Markers in Arbuscular Mycorrhizal Fungi (AMF) Research

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Book cover Molecular Markers in Mycology

Part of the book series: Fungal Biology ((FUNGBIO))

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Abstract

Arbuscular Mycorrhizal Fungi (AMF) are ecologically significant organisms for their positive interactions with the host plants. The genetics and phylogeny of this plant fungi symbiotic assemblage have long been a debated issue. Development of a suitable molecular marker is still a matter to resolve the appropriate diagnosis of these organisms that hurdles further research progress. Nuclear genome based markers are using quite often; however, proved difficult due to extreme variability. Mitochondrial DNA (mtDNA) based markers are supposed to be the alternative to nuclear DNA based markers. The use of mitochondrial large ribosomal subunit (mtLSU) and Cytochrome c oxidase (cox1) have now pave a new door to investigate the AMF phylogeny. However, very recent investigations revealed many possible limitations in restoring the homogeneity within mtDNA of a single AMF isolate that needs more comprehensive study. This chapter reviews the mitochondrial DNA based molecular markers in AMF diagnosis including the promises, pitfalls and project areas for future development in the field of AMF research.

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Acknowledgement

Authors are thankful to the Department of Biotechnology, for establishment of DBT-BIF centre and DBT-state Biotech Hub in the Biological Science & Technology Division, CSIR-NEIST Jorhat, which has been used for the present book chapter.

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Authors and Affiliations

  1. Biotechnology Group, Biological Science & Technology Division, CSIR-North East Institute of Science and Technology, Jorhat, Assam, 785006, India

    R. K. Sarma & Ratul Saikia

  2. Life Science Division, Institute of Advanced Study in Science and Technology (DST, Govt. India), Guwahati, Assam, 781035, India

    R. K. Sarma, Ratul Saikia & N. C. Talukdar

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  1. R. K. Sarma
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Correspondence to R. K. Sarma .

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Editors and Affiliations

  1. Department of Biotechnology, Mizoram University, Aizawl, Mizoram, India

    Bhim Pratap Singh

  2. Department of Biochemistry, National University of Ireland Galway, Galway, Ireland

    Vijai Kumar Gupta

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Sarma, R.K., Saikia, R., Talukdar, N.C. (2017). Mitochondrial DNA Based Molecular Markers in Arbuscular Mycorrhizal Fungi (AMF) Research. In: Singh, B.P., Gupta, V.K. (eds) Molecular Markers in Mycology. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-34106-4_11

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