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Direct Nucleotide Sequencing for Amplification Product Identification

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Advanced Techniques in Diagnostic Microbiology

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Abstract

Sanger sequencing technology remains an important technology for diagnostic microbiology. Pyrosequencing has simplified the procedure and makes rapid diagnoses possible. The following topics are discussed: (1) DNA sequence of hsp65 and recA gene sequence for identification of mycobacterial species, (2) DNA sequence of the internal transcribed spacer (ITS) region for bacterial and fungi identification, (3) nucleotide sequence of HIV and HCV for viral genome typing, and (4) sequence-based bacterial genome typing.

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Author information

Authors and Affiliations

  1. Department of Pathology, Hackensack University Medical Center, Hackensack, NJ, USA

    Tao Hong

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  1. Tao Hong
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Correspondence to Tao Hong .

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Editors and Affiliations

  1. Departments of Laboratory Medicine and Internal Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA

    Yi-Wei Tang

  2. Department of Pathology, Microbiology and Immunology and Medicine, Vanderbilt University Medical Center, Nashville, TN, USA

    Charles W. Stratton

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Hong, T. (2018). Direct Nucleotide Sequencing for Amplification Product Identification. In: Tang, YW., Stratton, C. (eds) Advanced Techniques in Diagnostic Microbiology. Springer, Cham. https://doi.org/10.1007/978-3-319-33900-9_19

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