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Mutagenesis and Transgenesis in Zebrafish

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The rights and wrongs of zebrafish: Behavioral phenotyping of zebrafish

Abstract

Over the last decades, the zebrafish has emerged as a powerful vertebrate model for studying development, diseases and behavior, and conducting high-throughput screens for therapeutic development. Large forward genetic screens have led to the generation of multiple mutant lines with developmental or behavioral defects, while transposon-based integration technologies have enabled the creation of transgenic lines essential for the functional analysis of cell and tissue movement, gene regulation, and gene function. The recent development of engineered endonucleases including ZFNs, TALENs and the CRISPR/Cas9 system has revolutionized reverse genetic approaches in zebrafish, allowing for the first time precise genome editing for targeted mutagenesis and transgenesis. In this chapter, we provide an overview of the different approaches used for mutagenesis and transgenesis in zebrafish, with an emphasis on the recent progress in targeted genetic manipulations. Examples of selected mutant and transgenic zebrafish strains are given to illustrate their growing utility for neurobehavioral phenomics and biological psychiatry.

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Abbreviations

Cas:

CRISPR-associated

CRISPR:

Clustered regulatory interspaced short palindromic repeats

DSB:

Double strand break

EENs:

Engineered endonucleases

ENU:

N-ethyl-N-nitrosourea

HDR:

Homology-directed repair

HR:

Homologous recombination

KI:

Knock-in

NHEJ:

Non homologous end joining

ssDNA:

Single-stranded DNA

TALEN:

Transcription-activator like effector nuclease

WT:

Wild-type

ZFN:

Zinc-finger nuclease

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Acknowledgements

We apologize to authors whose work was omitted from this article due to space limitations. Work in the F.E.P. laboratory is supported in part by R00NS083714 from the National Institute of Neurological Disorders and Stroke at the National Institute of Health.

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  1. Department of Biological Sciences, University of South Carolina, Columbia, SC, 29208, USA

    Fabienne E. Poulain

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  1. The International Zebrafish Neurosceince Research Consortium (ZNRC) and ZENEREI Research Center, Slidell, Louisiana, USA

    Allan V. Kalueff

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Poulain, F.E. (2017). Mutagenesis and Transgenesis in Zebrafish. In: Kalueff, A. (eds) The rights and wrongs of zebrafish: Behavioral phenotyping of zebrafish. Springer, Cham. https://doi.org/10.1007/978-3-319-33774-6_1

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