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The Uses of Somatic Embryogenesis for Genetic Transformation

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Somatic Embryogenesis: Fundamental Aspects and Applications

Abstract

Direct or indirect somatic embryogenesis protocols used for efficient Agrobacterium-, particle bombardment-, and chemical-mediated genetic transformation are revised in this chapter. Reported protocols for genetic transformation of important annual crops (corn, sorghum, rice, soybean, wheat, and sugarcane, among others) as well as perennials (Pinus, Picea, Vitis, Hevea, citrus, coffee, and several more), model plants (Nicotiana and Daucus), or pharmacologically attractive plants (opium poppy) are summarized. In general, a description of protocols developed with vectors bearing reporter and selectable gene markers is presented, and also the integration and expression of foreign genes for the protection of plant species against viruses, bacteria, fungi, and insects, or to enhance tolerance to herbicide or salt, and for producing recombinant proteins are described.

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Authors and Affiliations

  1. Departamento de Ingeniería Genética, and Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Irapuato, Km 9.6 Libramiento norte carretera Irapuato-León, 36821, Irapuato, Guanajuato, Mexico

    Neftalí Ochoa-Alejo

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  1. Neftalí Ochoa-Alejo
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Correspondence to Neftalí Ochoa-Alejo .

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Editors and Affiliations

  1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico

    Víctor M Loyola-Vargas

  2. Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Mexico

    Neftalí Ochoa-Alejo

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Ochoa-Alejo, N. (2016). The Uses of Somatic Embryogenesis for Genetic Transformation. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Somatic Embryogenesis: Fundamental Aspects and Applications. Springer, Cham. https://doi.org/10.1007/978-3-319-33705-0_23

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