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Dental Stem Cells: Regenerative Potential pp 27–56Cite as

Protocols for Dental-Related Stem Cells Isolation, Amplification and Differentiation

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Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

In this chapter we will discuss how to accommodate your research objectives to produce universal and scientifically relevant results by choosing a fitting research material and methods. We will start by exploring the character of various dental-related stem cell lineages through their lifecycle, following up with an overview of bioethics of stem cell research, the methods of tissue extraction and transport, tissue’s stem cells isolation, amplification, differentiation and differentiation evaluation; and finally arriving at methods of cryopreservation of the research material.

If I have seen further, it is by standing on the shoulders of giants. Isaac Newton

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Authors and Affiliations

  1. Department of Dentistry, Faculty of Medicine in Hradec Králové, Charles University in Prague, Sokolská 581, 500 05, Hradec Králové, Czech Republic

    Jakub Suchánek Ph.D. & Tereza Suchánková Kleplová M.D.

  2. Department of Histology and Embryology Faculty of Medicine in Hradec Kralove, Charles University in Prague, Sokolská 581, 500 05, Hradec Králové, Czech Republic

    Klára Zoe Browne M.Sc. & Yvona Mazurová C.Sc., M.D.

Authors
  1. Jakub Suchánek Ph.D.
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  2. Klára Zoe Browne M.Sc.
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  3. Tereza Suchánková Kleplová M.D.
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  4. Yvona Mazurová C.Sc., M.D.
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Corresponding author

Correspondence to Jakub Suchánek Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Biomedical Sciences, University of Padova, Padova, Italy

    Barbara Zavan

  2. Department of Neurosciences, University of Padova, Padova, Italy

    Eriberto Bressan

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© 2016 Springer International Publishing Switzerland

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Suchánek, J., Browne, K.Z., Kleplová, T.S., Mazurová, Y. (2016). Protocols for Dental-Related Stem Cells Isolation, Amplification and Differentiation. In: Zavan, B., Bressan, E. (eds) Dental Stem Cells: Regenerative Potential. Stem Cell Biology and Regenerative Medicine. Humana Press, Cham. https://doi.org/10.1007/978-3-319-33299-4_2

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