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In Vitro Models of Itch

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Pruritus

Abstract

Although itch is perceived through the brain, in vitro studies are possible and necessary to study its genesis. Cultures of keratinocytes or neurons or mast cells can be used. However, best models are provided by the co-culture of neurons and other cells: keratinocytes, mast cells, Langerhans cells, etc.… The best model is the co-culture of neurons and skin explants. In these models, representative biochemical, and sometimes electrophysiological, measurements can be performed.

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Abbreviations

AP:

Agonist Peptide

CGRP:

Calcitonin Gene-Related Peptide

CNS:

Central Nervous System

DRG:

Dorsal Root Gangli

MMP:

Matrix Metallo Protease

NGF:

Nerve Growth Factor

PAR:

Protease Activated Receptor

PSN:

Primary Sensory Neuron

Sema3A:

Semaphorin 3A

SP:

Substance P

TSLP:

Thymic Stromal Lymphopoietin

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Author information

Authors and Affiliations

  1. Laboratory of Neurosciences of Brest, University of Brest, 22 avenue Camille Desmoulins, Brest, 29200, France

    Nicolas Lebonvallet PhD

  2. Laboratory of Neurosciences of Brest, Department of Dermatology, University Hospital, University of Brest, Brest, France

    Laurent Misery MD, PhD

Authors
  1. Nicolas Lebonvallet PhD
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  2. Laurent Misery MD, PhD
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Correspondence to Nicolas Lebonvallet PhD .

Editor information

Editors and Affiliations

  1. Department of Dermatology, University of Brest, Brest, France

    Laurent Misery

  2. Center of Chronic Pruritus, University Hospital Münster, Münster, Nordrhein-Westfalen, Germany

    Sonja Ständer

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Lebonvallet, N., Misery, L. (2016). In Vitro Models of Itch. In: Misery, L., Ständer, S. (eds) Pruritus. Springer, Cham. https://doi.org/10.1007/978-3-319-33142-3_6

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  • DOI: https://doi.org/10.1007/978-3-319-33142-3_6

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