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Analysis of Decomposition Fluid Collected from Carcasses Decomposing in the Presence and Absence of Insects

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Part of the book series: Soil Forensics ((SOFO))

Abstract

Most decomposition studies investigate soft tissue degradation in the presence of insects, however several studies have shown that when insect activity is excluded from carcasses, the rate of decomposition slows down. The goal of this study was to explore the effect of insect activity on the chemical properties of decomposition fluid. Fluid was collected from pig (Sus scrofa) carcasses over the course of two summer trials (2011 and 2012) conducted in southern Ontario, Canada. The pH and conductivity were measured and fatty acids were analysed using Attenuated Total Reflectance- Infrared (ATR-IR) spectroscopy. Results were compared between insect inclusion, partial exclusion, and complete exclusion carcass groups. The results indicate that the presence of insects increases the pH and decreases the conductivity of decomposition fluid. Spectral fatty acid results did not appear to vary greatly between experimental groups. The overall levels were not sufficiently different between carcass groups to conclude that the presence of insects played an important role in the fatty acid degradation process.

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References

  • Amendt J, Campobasso CP, Gaudry E, Reiter C, LeBlanc HN, Hall MJR (2007) Best practice in forensic entomology–standards and guidelines. Int J Legal Med 121:90–104

    Article  PubMed  Google Scholar 

  • Anderson G, VanLaerhoven S (1996) Initial studies on insect succession on carrion in southwestern British Columbia. J Forensic Sci 41:617–625

    Article  Google Scholar 

  • Bernhardt T (n.d.) Canada’s ecozones. http://canadianbiodiversity.mcgill.ca/english/ecozones/index.htm. Accessed 20 Dec 2012

  • Dent BB, Forbes SL, Stuart BH (2004) Review of human decomposition processes in soil. Environ Geol 45:576–585

    Article  CAS  Google Scholar 

  • Evans WED (1963) The chemistry of death. Thomas, Springfield

    Google Scholar 

  • Fast PG (1966) A comparative study of the phospholipids and fatty acids of some insects. Lipids 1:209–215

    Article  CAS  PubMed  Google Scholar 

  • Ferguson HL, Jeffries DS (2012) Acid rain. http://www.thecanadianencyclopedia.com/articles/acid-rain. Accessed 9 May 2013

  • Fiedler S, Graw M (2003) Decomposition of buried corpses, with special reference to the formation of adipocere. Naturwissenschaften 90:291–300

    Article  CAS  PubMed  Google Scholar 

  • Flatten A, Bryhni E, Kohler A, Egelandsdal B, Isaksson T (2005) Determination of C22:5 and C22:6 marine fatty acids in pork fat with Fourier transform mid-infrared spectroscopy. Meat Sci 69:433–440

    Article  CAS  PubMed  Google Scholar 

  • Forbes SL, Dent BB, Stuart BH (2005a) The effect of soil type on adipocere formation. Forensic Sci Int 154:35–43

    Article  PubMed  Google Scholar 

  • Forbes SL, Stuart BH, Dadour IR, Dent BB (2004) A preliminary investigation of the stages of adipocere formation. J Forensic Sci 49:566–574

    Article  CAS  PubMed  Google Scholar 

  • Forbes SL, Stuart BH, Dent BB (2005b) The effect of the burial environment on adipocere formation. Forensic Sci Int 154:24–34

    Article  PubMed  Google Scholar 

  • Forbes SL, Wilson MEA, Stuart BH (2011) Examination of adipocere formation in a cold water environment. Int J Leg Med 125:643–650

    Article  Google Scholar 

  • Gill-King H (1997) Chemical and ultrastructural aspects of decomposition. In: Haglund WD, Sorg MH (eds) Forensic taphonomy: the postmortem fate of human remains. CRC Press, Florida, pp 93–105

    Google Scholar 

  • Goff ML (2009) Early post-mortem changes and stages of decomposition in exposed cadavers. Exp Appl Acarol 49:21–36

    Article  Google Scholar 

  • Hopkins DW, Wiltshire PEJ, Turner BD (2000) Microbial characteristics of soils from graves: an investigation at the interface of soil microbiology and forensic science. Appl Soil Ecol 14:283–288

    Article  Google Scholar 

  • Janaway RC, Percival SL, Wilson AS (2009) Decomposition of human remains. In: Percival SL (ed) Microbiology and aging. Humana Press, Totowa, pp 313–334

    Chapter  Google Scholar 

  • Kramer JM, Hulan HW (1978) A comparison of procedures to determine free fatty acids in rat heart. J Lipid Res 19:103–106

    CAS  PubMed  Google Scholar 

  • Likens G, Driscoll C, Buso D (1996) Long-term effects of acid rain: response and recovery of a forest ecosystem. Science 272:244–246

    Article  CAS  Google Scholar 

  • Megyesi MS, Nawrocki SP, Haskell NH (2005) Using accumulated degree-days to estimate the postmortem interval from decomposed human remains. J Forensic Sci 50:618–626

    Article  PubMed  Google Scholar 

  • Michaud J-P, Moreau G (2011) A statistical approach based on accumulated degree-days to predict decomposition-related processes in forensic studies. J Forensic Sci 56:229–232

    Article  PubMed  Google Scholar 

  • Myburgh J, L’Abbé EN, Steyn M, Becker PJ (2013) Estimating the postmortem interval (PMI) using accumulated degree-days (ADD) in a temperate region of South Africa. Forensic Sci Int 229:165.e1–165.e6

    Google Scholar 

  • Notter SJ, Stuart BH, Rowe R, Langlois N (2009) The initial changes of fat deposits during the decomposition of human and pig remains. J Forensic Sci 54:195–201

    Article  CAS  PubMed  Google Scholar 

  • O’Brien RC, Forbes SL, Meyer J, Dadour IR (2007) A preliminary investigation into the scavenging activity on pig carcasses in Western Australia. Forensic Sci Med Pathol 3:194–199

    Article  PubMed  Google Scholar 

  • Payne JA (1965) A summer carrion study of the Baby Pig Sus scrofa Linnaeus. Ecology 46:592–602

    Article  Google Scholar 

  • Pringle JK, Cassella JP, Jervis JR (2010) Preliminary soilwater conductivity analysis to date clandestine burials of homicide victims. Forensic Sci Int 198:126–133

    Article  PubMed  Google Scholar 

  • Reynold A, Cahill G (1965) Handbook of physiology: adipose tissue. American Physiological Society, Washington, DC

    Google Scholar 

  • Schoenly KG, Haskell NH, Mills DK, Bieme-Ndi C, Larsen K, Lee Y (2006) Recreating death’s acre in the school yard: using pig carcasses as model corpses to teach concepts of forensic entomology & ecological succession. Am Biol Teac 68:402–410

    Article  Google Scholar 

  • Simmons T, Adlam RE, Moffatt C (2010a) Debugging decomposition data–comparative taphonomic studies and the influence of insects and carcass size on decomposition rate. J Forensic Sci 55:8–13

    Article  PubMed  Google Scholar 

  • Simmons T, Cross PA, Adlam RE, Moffatt C (2010b) The influence of insects on decomposition rate in buried and surface remains. J Forensic Sci 55:889–892

    Article  PubMed  Google Scholar 

  • Stanley-Samuelson DW, Dadd RH (1983) Long-chain polyunsaturated fatty acids: patterns of occurrence in insects. Insect Biochem 13:549–558

    Article  CAS  Google Scholar 

  • Stanley-Samuelson DW, Jurenka RA, Cripps C, Blomquist GJ, De Renobales M (1988) Fatty acids in insects: composition, metabolism, and biological significance. Arch Insect Biochem Physiol 9:1–33

    Article  CAS  Google Scholar 

  • Stuart BH, Craft L, Forbes SL, Dent BB (2005) Studies of adipocere using attenuated total reflectance infrared spectroscopy. Forensic Sci Med Pathol 1(3):197–201

    Article  CAS  PubMed  Google Scholar 

  • Swann L, Chidlow GE, Forbes S, Lewis SW (2010a) Preliminary studies into the characterization of chemical markers of decomposition for geoforensics. J Forensic Sci 55:308–314

    Article  CAS  PubMed  Google Scholar 

  • Swann L, Forbes S, Lewis SW (2010b) Observations of the temporal variation in chemical content of decomposition fluid: a preliminary study using pigs as a model system. Aust J Forensic Sci 42:199–210

    Article  Google Scholar 

  • Swann LM, Forbes SL, Lewis SW (2010c) Analytical separations of mammalian decomposition products for forensic science: a review. Anal Chim Acta 682:9–22

    Article  CAS  PubMed  Google Scholar 

  • Swann LM, Forbes SL, Lewis SW (2010d) A capillary electrophoresis method for the determination of selected biogenic amines and amino acids in mammalian decomposition fluid. Talanta 81:1697–1702

    Article  CAS  PubMed  Google Scholar 

  • Turner B (2005) Blowfly maggots: the good, the bad and the ugly. Clin Pathol 14(2):81–85

    Article  Google Scholar 

  • Vass AA, Bass WM, Wolt JD, Foss JE, Ammons JT (1992) Time since death determinations of human cadavers using soil solution. J Forensic Sci 37:1236–1253

    Article  CAS  PubMed  Google Scholar 

  • Waugh A, Grant A (2010) Introduction to the chemistry of life. In: Premdas N, Hewat C (eds) Ross and Wilson anatomy and physiology in health and Illness, 11th edn. Churchill Livingstone, Toronto, 21

    Google Scholar 

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Correspondence to Shari L. Forbes .

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Comstock, J.L., LeBlanc, H.N., Forbes, S.L. (2016). Analysis of Decomposition Fluid Collected from Carcasses Decomposing in the Presence and Absence of Insects. In: Kars, H., van den Eijkel, L. (eds) Soil in Criminal and Environmental Forensics. Soil Forensics. Springer, Cham. https://doi.org/10.1007/978-3-319-33115-7_18

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