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Synthesis of Acyl-Acyl Carrier Proteins and Their Use in Studying Polyketide Synthase Enzymology

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Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases

Part of the book series: Springer Theses ((Springer Theses))

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Abstract

In this chapter, a simple and general method for the production of acyl-ACPs from a range of SNAC-thioesters is detailed, and their valuable usage as complete substrate mimics for studying the activity of a KS (PsyA KS1) domain, AT (PedD) domain and an AH (PedC) is highlighted.

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Authors and Affiliations

  1. School of Chemistry, University of Warwick, Coventry, UK

    Matthew Jenner

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  1. Matthew Jenner
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Correspondence to Matthew Jenner .

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Jenner, M. (2016). Synthesis of Acyl-Acyl Carrier Proteins and Their Use in Studying Polyketide Synthase Enzymology. In: Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases. Springer Theses. Springer, Cham. https://doi.org/10.1007/978-3-319-32723-5_5

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