Skip to main content
SpringerLink
Log in
Book cover

XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016 pp 264–268Cite as

Probing Collagen Nanocharacteristics After Low-Level Red Laser Irradiation

  • Conference paper
  • First Online:

Part of the book series: IFMBE Proceedings ((IFMBE,volume 57))

Abstract

The low level red light (LLRL) is increasingly being used in many fields of medicine, like wound healing and regeneration of damaged tissue. Although the positive effects of LLLT have been reported the effects of red light on nanoscale features of tissues still remain unknown and little is known about LLRL-collagen interactions. Thus, in this study, thin collagen films were formed as extracellular matrix (ECM) models and irradiated so as to investigate the influence of LLRL irradiation on collagen topography, mechanical properties and the influence in fibroblasts response. The alterations on topography and collagen Young’s modulus were recorded using Atomic Force Microscopy (AFM). Furthermore, fluorescence microscopy was applied for studying the fibroblast response cultured on the collagen models. The results demonstrated that LLRL had minor effects on collagen topography. However, it was demonstrated that the LLRL altered the mechanical properties of the fibrils (the standard mechanical pattern due to the D – band periodicity was affected). Additionally, it was shown that fibroblasts cultured on LLRL-irradiated collagen thin films responded to LRLL. The above results provide new insights into the underlying nanoscale mechanisms of LLRL.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Avci P, Gupta, A, Sadasivam M et al. (2013) Low-level laser (light) therapy (LLLT) in skin: stimulating, healing, restoring. Semin Cutan Med Surg 32:41-52

    Google Scholar 

  2. Chung H, Dai T, Sharma S K et al. (2012) The nuts and bolts of low-level laser (Light) therapy. Ann Biomed Eng 40:516-533

    Google Scholar 

  3. Gupta, A, Avci P, Sadasivam M et al. (2013) Shining light on nanotechnology to help repair and regeneration. Biotechnol Adv 31:607-631

    Google Scholar 

  4. Seaton, E D, Mouser P E, Charakida, A et al. (2006) Investigation of the mechanism of action of nonablative pulsed-dye laser therapy in photorejuvenation and inflammatory acne vulgaris. Brit J of Dermatol 155:748-755

    Google Scholar 

  5. Mandel A and Hamblin, M R (2012) A renaissance in low-level laser (light) therapy – LLLT. Photonics Lasers Med 1:231-234

    Google Scholar 

  6. Farivar S, Malekshahabi T and Shiari R (2014) Biological effects of low level laser therapy. J Laser Med Sci 5:58-62

    Google Scholar 

  7. AlGhamdi K M, Kumar A and Moussa N (2012) A Low-level laser therapy: A useful technique for enhancing the proliferation of various cultured cells. Laser Med Sci 27:237-249

    Google Scholar 

  8. Gupta A, Dai T and Hamblin M R (2014) Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice. Laser Med Sci 29:257-265

    Google Scholar 

  9. Hawkins D, Houreld N and Abrahamse H (2005) Low level laser therapy (LLLT) as an effective therapeutic modality for delayed wound healing. Ann N Y Acad Sci 1056:486-493

    Google Scholar 

  10. Fiório F B, Albertini R, Leal-Junior E C P et al. (2013) Effect of low-level laser therapy on I and III collagen and inflammatory cells in rats with induced third-degree burns. Laser Med Sci 29:1-7

    Google Scholar 

  11. Pugliese L S, Medrado A P, Reis S R et al. (2003) The influence of low-level laser therapy on biomodulation of collagen and elastic fibers. Pesqui Odontol Bras 17:307-313

    Google Scholar 

  12. Frozanfar A, Ramezani M, Rahpeyma A et al. (2-13) The effects of low level laser therapy on the expression of collagen type I gene and proliferation of human gingival fibroblasts (HGF3-PI 53): In vitro study. Iran J Basic Med Sci 16:1071-1074

    Google Scholar 

  13. De Souza T O F, Mesquita D A, Ferrari R A M et al. (2011) Phototherapy with low-level laser affects the remodeling of types i and III collagen in skeletal muscle repair. Laser Med Sci 26:803-814

    Google Scholar 

  14. Martignago C C S, Oliveira R F, Pires-Oliveira, D A A et al. (2015) Effect of low-level laser therapy on the gene expression of collagen and vascular endothelial growth factor in a culture of fibroblast cells in mice. Laser Med Sci 30:203-208

    Google Scholar 

  15. de Jesus J F, Spadacci-Morena D D, dos Anjos Rabelo N D et al. (2015) Low-level laser therapy in IL-1β, COX-2, and PGE2 modulation in partially injured Achilles tendon. Laser Med Sci 30:153-158

    Google Scholar 

  16. Fillipin L I, Mauriz J L, Vedovelli K et al. (2005) Low-level laser therapy (LLLT) prevents oxidative stress and reduces fibrosis in rat traumatized Achilles tendon. Laser Surg Med 37:293-300

    Google Scholar 

  17. Guerra F D R, Vieira C P, Dos Santos De Almeida et al. (2014) Pulsed LLLT improves tendon healing in rats: A biochemical, organizational, and functional evaluation. Laser Med Sci 29:805-811

    Google Scholar 

  18. Bossini P S, Rennó, A C M, Ribeiro D A et al. (2012) Low level laser therapy (830nm) improves bone repair in osteoporotic rats: Similar outcomes at two different dosages. Exp Gerontol 47:136-142

    Google Scholar 

  19. Wood V T, Pinfildi C E, Neves M A I. et al. (2010) Collagen changes and realignment induced by low-level laser therapy and low-intensity ultrasound in the calcaneal tendon Laser Surg Med 42:559-565

    Google Scholar 

  20. Hawkins D H, Abrahamse H (2006) The role of laser fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following Helium-Neon laser irradiation. Laser Surg Med 38:74-83

    Google Scholar 

  21. Fushimi T, Inui S, Nakajima T et al. (2012) Green light emitting diodes accelerate wound healing: Characterization of the effect and its molecular basis in vitro and in vivo. Wound Repair Regen 20:226-235

    Google Scholar 

  22. Peplow PV, Chung T Y, and Baxter G D (2012) Laser photostimulation (660nm) of wound healing in diabetic mice is not brought about by ameliorating diabetes. Laser Surg Med 44:26-29

    Google Scholar 

  23. Kadler K E, Baldock C, Bella J et al. (2007) Collagens at a glance. J Cell Sci 120:1955-1958

    Google Scholar 

  24. Fratzl P (2008) Collagen structure and mechanics. Springer, New York

    Google Scholar 

  25. Stylianou A, Yova D,Politopoulos K (2012) Atomic force microscopy surface nanocharacterization of UV-irradiated collagen thin films. IEEE proc. IEEE 12th International Conference BIBE, 2012, Larnaca, Cyprus pp 602-607 DOI: 10.1109/BIBE.2012.6399741

    Google Scholar 

  26. Kontomaris S V, Stylianou A, Yova D et al. (2014) Influence of UV Irradiation on Collagen Fibrils Using AFM Imaging and Nanoindentation Methods. Scanning 37:101-111

    Google Scholar 

  27. Minary-Jolandan M, Yu M F (2009) Nanomechanical heterogeneity in the gap and overlap regions of type I collagen fibrils with implications for bone heterogeneity. Biomacromolecules 10(9):2565-2570

    Google Scholar 

  28. Wenger M P E, Bozec L, Horton M A et al. (2007) Mechanical properties of collagen fibrils. Biophysic J 93:1255-1263

    Google Scholar 

  29. Stylianou A, Kontomaris S V, Yova D (2014) Assessing Collagen Nanoscale Thin Films Heterogeneity by AFM Multimode Imaging and Nanoindetation for NanoBioMedical Applications. Micro and Nanosystems 6:95-102

    Google Scholar 

  30. Stylianou A, Kontomaris S B, Kyriazi M et al. (2010) Surface characterization of collagen films by atomic force microscopy. IFMBE Proc. vol. 29 XII Mediterranean Conference on Med. Phys. & Biomed. Eng. Chalkidiki, Greece, 2010, pp 612-615

    Google Scholar 

  31. Darling E M (2011) Force scanning: A rapid, high-resolution approach for spatial mechanical property mapping. Nanotechnology 22:175707

    Google Scholar 

  32. Stylianou A, Yova D (2013) Surface nanoscale imaging of collagen thin films by Atomic Force Microscopy. Mater Sci Eng C Mater Biol Appl 33:2947-2957

    Google Scholar 

  33. Stylianou A, Yova D, Alexandratou E (2014) Investigation of the influence of UV irradiation on collagen thin films by AFM imaging. Mater Sci Eng C Mater Biol Appl 45:455-468

    Google Scholar 

  34. Stylianou A, Politopoulos K, Kyriazi et al. (2011) Combined information from AFM imaging and SHG signal analysis of collagen thin films. Biomed Signal Proces 6:307-313

    Google Scholar 

  35. Stylianou A, Yova D, Alexandratou E (2013) Nanotopography of collagen thin films in correlation with fibroblast response. J Nanophotonics 7:073590

    Google Scholar 

  36. Alexandratou E, Yova D, Handris P et al. (2002) Human fibroblast alterations induced by low power laser irradiation at the single cell level using confocal microscopy. Photochem Photobiol Sci 1:547-552

    Google Scholar 

  37. Horcas I, Fernández R, Gómez-Rodríguez J M et al. (2007) WSXM: A software for scanning probe microscopy and a tool for nanotechnology Rev Sci Instrum 78:013705

    Google Scholar 

  38. Kurland N E, Drira Z and Yadavalli V K (2012) Measurement of nanomechanical properties of biomolecules using atomic force microscopy. Micron 43:116-128

    Google Scholar 

  39. Yadavalli V K, Svintradze D V, Pidaparti R M (2010) Nanoscale measurements of the assembly of collagen to fibrils. Int J Biol Macromol 46:458-464

    Google Scholar 

  40. Strasser S, Zink A, Janko M, et al. (2007) Structural investigations on native collagen type I fibrils using AFM. Biochem Biophys Res Commun 354:27-32

    Google Scholar 

  41. Heim A J, Matthews WG, Koob T J (2006) Determination of the elastic modulus of native collagen fibrils via radial indentation. Appl Phys Lett 89:181902

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biomedical Optics and Applied Biophysics Laboratory, School of Electrical and Computer Engineering, National Technical University of Athens, 91, Aglantzias Ave., Athens, Greece

    Andreas Stylianou, Stylianos Vasileios Kontomaris & Dido Yova

  2. Cancer Biophysics Laboratory, Department of Mechanical and Manufacturing Engineering, Faculty of Engineering, University of Cyprus, Nicosia, Cyprus

    Andreas Stylianou

Authors
  1. Andreas Stylianou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Stylianos Vasileios Kontomaris
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dido Yova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Andreas Stylianou .

Editor information

Editors and Affiliations

  1. Department of Computer Science and Engineering, Frederick University, Nicosia, Cyprus

    Efthyvoulos Kyriacou

  2. Biomedical Research Foundation, Nicosia, Cyprus

    Stelios Christofides

  3. Department of Computer Science, University of Cyprus, Nicosia, Cyprus

    Constantinos S. Pattichis

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer International Publishing Switzerland

About this paper

Cite this paper

Stylianou, A., Kontomaris, S.V., Yova, D. (2016). Probing Collagen Nanocharacteristics After Low-Level Red Laser Irradiation. In: Kyriacou, E., Christofides, S., Pattichis, C. (eds) XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016. IFMBE Proceedings, vol 57. Springer, Cham. https://doi.org/10.1007/978-3-319-32703-7_53

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-32703-7_53

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-32701-3

  • Online ISBN: 978-3-319-32703-7

  • eBook Packages: EngineeringEngineering (R0)

Publish with us

Policies and ethics

Search

Navigation