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Algae-Based Biopharmaceuticals pp 109–120Cite as

Other Biopharmaceuticals Produced in Algae

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Abstract

Algae species are considered the next generation hosts for the production of biopharmaceuticals (BFs) offering several attributes. In the present book specific groups of BFs produced in algae have been presented including antibodies, immunotoxins, cytokines, growth factors, and vaccines. In this chapter the case of some algae-made BFs that do not fall in these categories is analyzed, including the following: lactoferricin, neutrophil peptide-1, flounder growth hormone, erythropoietin, human growth hormone, soybean Kunitz trypsin inhibitor, and fibronectin domains. Except for erythropoietin as well as the fibronectin domain 10FN3 engineered at the chloroplast level, all the candidates have been successfully produced in algae and constitute attractive candidates for further evaluations, e.g. completing preclinical evaluation and initiating clinical trials.

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References

  • Agerberth B, Gudmundsson GH (2006) Host antimicrobial defence peptides in human disease. Curr Top Microbiol Immunol 306:67–90

    CAS  PubMed  Google Scholar 

  • Bai LL, Yin WB, Chen YH, Niu LL, Sun YR, Zhao SM, Yang FQ, Wang RR, Wu Q, Zhang XQ, Hu ZM (2013) A new strategy to produce a defensin: stable production of mutated NP-1 in nitrate reductase-deficient Chlorella ellipsoidea. PLoS One 8(1):e54966

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bossard C, Van den Berghe L, Laurell H, Castano C, Cerutti M, Prats AC, Prats H (2004) Antiangiogenic properties of fibstatin, an extracellular FGF-2-binding polypeptide. Cancer Res 64(20):7507–7512

    Article  CAS  PubMed  Google Scholar 

  • Brogden KA (2005) Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nat Rev Microbiol 3(3):238–250

    Article  CAS  PubMed  Google Scholar 

  • Castilho A, Neumann L, Gattinger P, Strasser R, Vorauer-Uhl K, Sterovsky T, Altmann F, Steinkellner H (2013) Generation of biologically active multi-sialylated recombinant human EPOFc in plants. PLoS One 8(1):e54836

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Celik E, Calik P (2012) Production of recombinant proteins by yeast cells. Biotechnol Adv 30(5):1108–1118

    Article  CAS  PubMed  Google Scholar 

  • Chai XJ, Chen HX, Xu WQ, Xu YW (2013) Expression of soybean Kunitz trypsin inhibitor gene SKTI in Dunaliella salina. J Appl Phycol 25(1):139–144

    Article  CAS  Google Scholar 

  • Chen Y, Wang YQ, Sun YR, Zhang LM, Li WB (2001) Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells. Curr Genet 39(5–6):365–370

    Article  CAS  PubMed  Google Scholar 

  • Cheon BY, Kim HJ, Oh KH, Bahn SC, Ahn JH, Choi JW, Ok SH, Bae JM, Shin JS (2004) Overexpression of human erythropoietin (EPO) aVects plant morphologies: retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis. Transgenic Res 13(6):541–549

    Article  CAS  PubMed  Google Scholar 

  • de Hostos EL, Togasaki RK, Grossman A (1988) Purification and biosynthesis of a derepressible periplasmic arylsulfatase from Chlamydomonas reinhardtii. J Cell Biol 106(1):29–37

    Article  PubMed  Google Scholar 

  • Eichler-Stahlberg A, Weisheit W, Ruecker O, Heitzer M (2009) Strategies to facilitate transgene expression in Chlamydomonas reinhardtii. Planta 229(4):873–883

    Article  CAS  PubMed  Google Scholar 

  • Elliott S, Lorenzini T, Asher S, Aoki K, Brankow D, Buck L, Busse L, Chang D, Fuller J, Grant J, Hernday N, Hokum M, Hu S, Knudten A, Levin N, Komorowski R, Martin F, Navarro R, Osslund T, Rogers G, Rogers N, Trail G, Egrie J (2003) Enhancement of therapeutic protein in vivo activities through glycoengineering. Nat Biotechnol 21(4):414–421

    Article  CAS  PubMed  Google Scholar 

  • Fischer S, Handrick R, Otte K (2015) The art of CHO cell engineering: a comprehensive retrospect and future perspectives. Biotechnol Adv 33(8):1878–1896

    Article  CAS  PubMed  Google Scholar 

  • Goeddel DV, Heyneker HL, Hozumi T, Arentzen R, Itakura K, Yansura DG, Ross MJ, Miozzari G, Crea R, Seeburg PH (1979) Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone. Nature 281:544–548

    Article  CAS  PubMed  Google Scholar 

  • Hancock RE (1997) Peptide antibiotics. Lancet 349(9049):418–422

    Article  CAS  PubMed  Google Scholar 

  • Hawkins RL, Nakamura M (1999) Expression of human growth hormone by the eukaryotic alga, Chlorella. Curr Microbiol 38(6):335–341

    Article  CAS  PubMed  Google Scholar 

  • Heinzelman P, Krais J, Ruben E, Pantazes R (2015) Engineering pH responsive fibronectin domains for biomedical applications. J Biol Eng 9:6

    Article  PubMed  PubMed Central  Google Scholar 

  • Hoshijima M, Hattori T, Inoue M, Araki D, Hanagata H, Miyauchi A, Takigawa M (2006) CT domain of CCN2/CTGF directly interacts with fibronectin and enhances cell adhesion of chondrocytes through integrin alpha5beta1. FEBS Lett 580(5):1376–1382

    Article  CAS  PubMed  Google Scholar 

  • Høybye C, Cohen P, Hoffman AR, Ross R, Biller BM, Christiansen JS, Growth Hormone Research Society (2015) Status of long-acting-growth hormone preparations–2015. Growth Horm IGF Res 25(5):201–206

    Article  PubMed  Google Scholar 

  • Jelkmann W (2010) Biosimilar epoetins and other ‘follow-on’ biologics: update on the European experiences. Am J Hematol 85(10):771–780

    Article  PubMed  Google Scholar 

  • Jelkmann W (2013) Physiology and pharmacology of erythropoietin. Transfus Med Hemother 40(5):302–309

    Article  PubMed  PubMed Central  Google Scholar 

  • Jones RL, Robinson DG (1989) Protein secretion in plants. New Phytol 111:567–597

    Article  CAS  Google Scholar 

  • Kajino T, Saito Y, Asami O, Yamada Y, Hirai M, Udaka S (1997) Extracellular production of an intact and biologically active human growth hormone by the Bacillus brevis system. J Industr Microbiol Biotechnol 19(4):227–231

    Article  CAS  Google Scholar 

  • Kim DH, Kim YT, Cho JJ, Bae JH, Hur SB, Hwang I, Choi TJ (2002) Stable integration and functional expression of flounder growth hormone gene in transformed microalga, Chlorella ellipsoidea. Mar Biotechnol 4(1):63–73

    Article  CAS  PubMed  Google Scholar 

  • Kobayashi H (2013) Prevention of cancer and inflammation by soybean protease inhibitors. Front Biosci (Elite Ed) 5:966–973

    Article  Google Scholar 

  • Koide A, Bailey CW, Huang X, Koide S (1998) The fibronectin type III domain as a scaffold for novel binding proteins. J Mol Biol 284(4):1141–1151

    Article  CAS  PubMed  Google Scholar 

  • Koide A, Koide S (2007) Monobodies: antibody mimics based on the scaffold of the fibronectin type III domain. Methods Mol Biol 352:95–109

    CAS  PubMed  Google Scholar 

  • Li SS, Tsai HJ (2009) Transgenic microalgae as a non-antibiotic bactericide producer to defend against bacterial pathogen infection in the fish digestive tract. Fish Shellfish Immunol 26(2):316–325

    Article  PubMed  Google Scholar 

  • Lipovsek D (2010) Adnectins: engineered target-binding protein therapeutics. Protein Eng Des Sel 24(1–2):3–9

    PubMed  PubMed Central  Google Scholar 

  • Matsumoto S, Ikura K, Ueda M, Sasaki R (1995) Characterization of a human glycoprotein (erythropoietin) produced in cultured tobacco cells. Plant Mol Biol 27(6):1163–1172

    Article  CAS  PubMed  Google Scholar 

  • Miyakawa Y, Ratnakar P, Rao AG, Costello ML, Mathieu-Costello O, Lehrer RI, Catanzaro A (1996) In vitro activity of the antimicrobial peptides human and rabbit defensins and porcine leukocyte protegrin against Mycobacterium tuberculosis. Infect Immun 64(3):926–932

    CAS  PubMed  PubMed Central  Google Scholar 

  • Mooradian DL, Lucas RC, Weatherbee JA, Furcht LT (1989) Transforming growth factor-beta-1 binds to immobilized fibronectin. J Cell Biochem 41(4):189–200

    Article  CAS  PubMed  Google Scholar 

  • Moryyama S, Yamamoto H, Sugimoto S, Abe T, Hirano T, Kawauchi H (1993) Oral administration of recombinant salmon growth hormone to rainbow trout, Onchorhynchus mykiss. Aquaculture 112(1):99–106

    Article  Google Scholar 

  • Parsons J, Altmann F, Arrenberg CK, Koprivova A, Beike AK, Stemmer C, Gorr G, Reski R, Decker EL (2012) Moss-based production of asialo-erythropoietin devoid of Lewis A and other plant-typical carbohydrate determinants. Plant Biotechnol J 10(7):851–861

    Article  CAS  PubMed  Google Scholar 

  • Parsons J, Altmann F, Graf M, Stadlmann J, Reski R, Decker EL (2013) A gene responsible for prolyl-hydroxylation of moss-produced recombinant human erythropoietin. Sci Rep 3:3019

    Article  PubMed  PubMed Central  Google Scholar 

  • Rahman S, Patel Y, Murray J, Patel KV, Sumathipala R, Sobel M, Wijelath ES (2005) Novel hepatocyte growth factor (HGF) binding domains on fibronectin and vitronectin coordinate a distinct and amplified Met-integrin induced signalling pathway in endothelial cells. BMC Cell Biol 6(1):8

    Article  PubMed  PubMed Central  Google Scholar 

  • Rasala BA, Muto M, Lee PA, Jager M, Cardoso RM, Behnke CA, Kirk P, Hokanson CA, Crea R, Mendez M, Mayfield SP (2010) Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii. Plant Biotechnol J 8(6):719–733

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rho J, Mead JR, Wright WS, Brenner DE, Stave JW, Gildersleeve JC, Lampe PD (2014) Discovery of sialyl Lewis A and Lewis X modified protein cancer biomarkers using high density antibody arrays. J Proteomics 96:291–299

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Richards J, Miller M, Abend J, Koide A, Koide S, Dewhurst S (2003) Engineered fibronectin type III domain with a RGDWXE sequence binds with enhanced affinity and specificity to human alphavbeta3 integrin. J Mol Biol 326(5):1475–1488

    Article  CAS  PubMed  Google Scholar 

  • Rosales-Mendoza S, Paz-Maldonado LM, Soria-Guerra RE (2012) Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives. Plant Cell Rep 31(3):479–494

    Article  CAS  PubMed  Google Scholar 

  • Sinha S, Cheshenko N, Lehrer RI, Herold BC (2003) NP-1, a rabbit alpha-defensin, prevents the entry and intercellular spread of herpes simplex virus type 2. Antimicrob Agents Chemother 47(2):494–500

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Smith EM, Mitsi M, Nugent MA, Symes K (2009) PDGF-A interactions with fibronectin reveal a critical role for heparan sulfate in directed cell migration during Xenopus gastrulation. Proc Natl Acad Sci U S A 106(51):21683–21688

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sun YL, Kuan TC, Lin YJ, Chou YC, Lin CS (2010) Construction and expression of rabbit neutrophil peptide-1 gene in Escherichia coli. Ann Microbiol 60:329–334

    Article  CAS  Google Scholar 

  • Terpe K (2006) Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol 72(2):211–222

    Article  CAS  PubMed  Google Scholar 

  • Wang YQ, Chen Y, Sun YR (2005) Isolation and characterization of a nitrate reductase deficient mutant of Chlorella ellipsoidea (Chlorophyta). J Appl Phycol 17(4):281–286

    Article  CAS  Google Scholar 

  • Wijelath ES, Rahman S, Namekata M, Murray J, Nishimura T, Mostafavi-Pour Z, Patel Y, Suda Y, Humphries MJ, Sobel M (2006) Heparin-II domain of fibronectin is a vascular endothelial growth factor-binding domain—enhancement of VEGF biological activity by a singular growth factor/matrix protein synergism. Circ Res 99(8):853–860

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Weise A, Altmann F, Rodriguez-Franco M, Sjoberg ER, Bäumer W, Launhardt H, Kietzmann M, Gorr G (2007) High-level expression of secreted complex glycosylated recombinant human erythropoietin in the Physcomitrella D-fuc-t D-xyl-t mutant. Plant Biotechnol J 5(3):389–401

    Article  CAS  PubMed  Google Scholar 

  • Xu Q, Yan B, Li S, Duan C (2004) Fibronectin binds insulin-like growth factor-binding protein 5 and abolishes its ligand-dependent action on cell migration. J Biol Chem 279(6):4269–4277

    Article  CAS  PubMed  Google Scholar 

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  1. Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico

    Sergio Rosales-Mendoza

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  1. Sergio Rosales-Mendoza
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Rosales-Mendoza, S. (2016). Other Biopharmaceuticals Produced in Algae. In: Algae-Based Biopharmaceuticals. Springer, Cham. https://doi.org/10.1007/978-3-319-32232-2_7

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