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Manual of Cardiovascular Proteomics pp 59–104Cite as

Organelle, Protein and Peptide Fractionation in Cardiovascular Proteomics

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Abstract

Proteomics experiments are as diverse as the scientists who perform them. Goals range from the desire to understand a subcellular structure or an individual protein in greater depth, to identification of novel protein-protein interactions. Or perhaps, the goal is to obtain a global protein abundance profile from an animal model of cardiovascular disease or from patient biopsies. Regardless of scale or objective, inevitably, the tools of organelle isolation, protein purification or peptide fractionation will play an integral role. In this chapter, we survey both time-honored and state-of-the-art fractionation techniques, with an emphasis on underlying physical and chemical principles.

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Funding

MRM was supported by an NIH T32 grant, 5T32HL007576-30. SF was supported by an NIH/NHLBI R01 HL130424 and the Nora Eccles Treadwell Foundation. DBF was supported by an American Heart Association National Scientist Development Grant 12SDG12060056, NIH/NHLBI R21HL108052 and the Zegar Family Foundation

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Authors and Affiliations

  1. Department of Cardiology, Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, Salt Lake City, UT, USA

    Mickey R. Miller PhD

  2. Cardiology Division, Department of Internal Medicine, The Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, 95 South 2000 East, Room 101, Salt Lake City, UT, 84112, USA

    Sarah Franklin PhD

  3. Department of Biochemistry, University of Utah, Salt Lake City, UT, USA

    Sarah Franklin PhD

  4. Laboratory of Cardiovascular Biochemistry, Division of Cardiology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    D. Brian Foster PhD

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  1. Mickey R. Miller PhD
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  2. Sarah Franklin PhD
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  3. D. Brian Foster PhD
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Correspondence to Sarah Franklin PhD or D. Brian Foster PhD .

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Editors and Affiliations

  1. Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Giulio Agnetti

  2. Dept. of Physiology & Biophysics, University Mississippi Medical Center, Jackson, Mississippi, USA

    Merry L. Lindsey

  3. Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    D. Brian Foster

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Miller, M.R., Franklin, S., Foster, D.B. (2016). Organelle, Protein and Peptide Fractionation in Cardiovascular Proteomics. In: Agnetti, G., Lindsey, M., Foster, D. (eds) Manual of Cardiovascular Proteomics. Springer, Cham. https://doi.org/10.1007/978-3-319-31828-8_4

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