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Post-translational Modifications in the Cardiovascular Proteome

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Manual of Cardiovascular Proteomics

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Abstract

The analysis of post-translational modifications is critical for understanding the regulation of protein function in the heart. These small, often charged, groups are added to a protein’s structure to modulate its activity, localization or associations. The development of proteomic technologies has greatly improved the identification and subsequent characterization of these modifications. However, due to the complex nature of the cardiovascular proteome, a particular post-translational modification may represent only a tiny fraction of the milieu. Additionally, some modifications are too labile for mass spectrometry analysis. To address these limitations, a variety of techniques and strategies have been developed to specifically target and improve the detection of these modifications. In the following chapter, we review the challenges and solutions to identifying several prominent post-translational modifications in the cardiovascular system.

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Authors and Affiliations

  1. Department of Anesthesiology, Pharmacology and Therapeutics, University of British Columbia, Vancouver, BC, Canada

    Christopher I. Murray PhD

  2. Life Sciences Centre, 2350 Health Science Mall, Room 2.303, Vancouver, BC, V6T 1Z3, Canada

    Christopher I. Murray PhD

  3. Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 21218, USA

    Heaseung Sophia Chung MS

  4. Division of Cardiology, Department of Medicine, Johns Hopkins University, Johns Hopkins School of Medicine, Baltimore, MD, 21218, USA

    Kyriakos N. Papanicolaou PhD

  5. Laboratory of Cardiovascular Biochemistry, Division of Cardiology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    D. Brian Foster PhD

  6. Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD, 21205, USA

    Mark J. Kohr PhD

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  1. Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Giulio Agnetti

  2. Dept. of Physiology & Biophysics, University Mississippi Medical Center, Jackson, Mississippi, USA

    Merry L. Lindsey

  3. Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    D. Brian Foster

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Murray, C.I., Chung, H.S., Papanicolaou, K.N., Foster, D.B., Kohr, M.J. (2016). Post-translational Modifications in the Cardiovascular Proteome. In: Agnetti, G., Lindsey, M., Foster, D. (eds) Manual of Cardiovascular Proteomics. Springer, Cham. https://doi.org/10.1007/978-3-319-31828-8_13

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