Abstract
RNA-protein interactions are central to cellular homeostasis and control every aspect of RNA metabolism in the cell. A variety of in vitro and in vivo techniques have been developed in the last three decades to study these interactions. Here we provide a brief review of the currently available techniques as well as an in-depth discussion of experimental and data analysis considerations for Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP). The RNA-protein networks interrogated by PAR-CLIP and other high-throughput methods will greatly enhance our understanding of post-transcriptional gene regulation in cellular homeostasis and disease.
Authors contributed equally and are listed alphabetically.
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Dutka, T., Sarshad, A.A., Hafner, M. (2016). PAR-CLIP: A Genomic Technique to Dissect RNA-Protein Interactions. In: Aransay, A., LavÃn Trueba, J. (eds) Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing. Springer, Cham. https://doi.org/10.1007/978-3-319-31350-4_11
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