Abstract
Fresh histological tissues cannot be used “as is” and must undergo special processing to turn them into a suitable experimental material: tissues need to be first chemically fixed and then cut into thin sections using special histological instruments. Fixation and tissue sectioning are critical to the success of IHC staining. If they are not done correctly, staining quality can be badly compromised regardless of the use of high-quality primary and secondary antibodies , buffers , and detection reagents.
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© 2016 Springer International Publishing Switzerland
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Kalyuzhny, A.E. (2016). Preparing Tissues for IHC Staining. In: Immunohistochemistry. Techniques in Life Science and Biomedicine for the Non-Expert. Springer, Cham. https://doi.org/10.1007/978-3-319-30893-7_6
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DOI: https://doi.org/10.1007/978-3-319-30893-7_6
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Publisher Name: Springer, Cham
Print ISBN: 978-3-319-30891-3
Online ISBN: 978-3-319-30893-7
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