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Approaches to Inactivate Genes in Zebrafish

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Cancer and Zebrafish

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 916))

Abstract

Animal models of tumor initiation and tumor progression are essential components toward understanding cancer and designing/validating future therapies. Zebrafish is a powerful model for studying tumorigenesis and has been successfully exploited in drug discovery. According to the zebrafish reference genome, 82 % of disease-associated genes in the Online Mendelian Inheritance in Man (OMIM) database have clear zebrafish orthologues. Using a variety of large-scale random mutagenesis methods developed to date, zebrafish can provide a unique opportunity to identify gene mutations that may be associated with cancer predisposition. On the other hand, newer technologies enabling targeted mutagenesis can facilitate reverse cancer genetic studies and open the door for complex genetic analysis of tumorigenesis. In this chapter, we will describe the various technologies for conducting genome editing in zebrafish with special emphasis on the approaches to inactivate genes.

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Acknowledgments

This work is supported by the National Institutes of Health (J.-R. J. Yeh, GM088040 and CA140188; J. M. Parant, NS092105) and the Hassenfeld Scholar’s Award (J.-R. J. Yeh).

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Authors and Affiliations

  1. Department of Pharmacology and Toxicology, UAB Comprehensive Cancer Center, University of Alabama at Birmingham School of Medicine, Birmingham, AL, 35294, USA

    John M. Parant

  2. Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA, 02129, USA

    Jing-Ruey Joanna Yeh

  3. Department of Medicine, Harvard Medical School, Boston, MA, 02115, USA

    Jing-Ruey Joanna Yeh

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  1. John M. Parant
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  2. Jing-Ruey Joanna Yeh
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Correspondence to John M. Parant or Jing-Ruey Joanna Yeh .

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  1. Molecular Pathology and Cancer Center, Massachusetts General Hospital, CHARLESTOWN, Massachusetts, USA

    David M. Langenau

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Parant, J.M., Yeh, JR.J. (2016). Approaches to Inactivate Genes in Zebrafish. In: Langenau, D. (eds) Cancer and Zebrafish. Advances in Experimental Medicine and Biology, vol 916. Springer, Cham. https://doi.org/10.1007/978-3-319-30654-4_3

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