Abstract
Understanding how cells behave and interact with surrounding cells, tissues, microorganisms and all types of biological, biochemical and biomechanical cues from their environment, constitutes a relevant research challenge and requires the support, not only of advanced manipulation and imaging technologies, but also of specifically designed biomedical microsystems with micrometric and even nanometric details for enabling interactions at a cellular and molecular level. These types of microsystems, together with the use of advanced design and manufacturing strategies for their efficient development, constitute the core topic of present Handbook. Biomedical microsystems aimed at interacting with and studying the behavior of cells, include: dishes for 2D culture, microsystems for studying cells under chemical gradients, electrophoretic microsystems, multi-culture platforms and devices for cell co-culture and dynamic bioreactors or cell culture platforms. This chapter provides an introduction to these different types of biomedical microdevices, illustrating them by means of different cases of study. Main current research trends are also outlined. Other emerging and possibly more complex microsystems for interacting with cells and controlling their behavior and fate, even with the potential of constructing whole tissues and organs from cultured cells, are covered in depth in Chaps. 13–23.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Abbott NJ, Dolman DE, Drndarski S, Fredriksson SM (2012) An improved in vitro blood-brain barrier model: rat brain endothelial cells co-cultured with astrocytes. Methods Mol Biol 814:415–430
Apicella A, Aversa A (2012) A biomimetic and biomechanical approach for tissue engineering: hybrid nanomaterials and a piezoelectric tunable bending apparatus for mechanically stimulated osteoblast cells growth. Biodevices 2012:280–285
Chan CKF, Chen CC, Luppen CA, Kraft DL, Kim JB, De Boer A, Wei K, Helms JA, Kuo CJ, Weissman IL (2009) Endochondral ossification is required for hematopoietic stem cell niche formation. Nature 457(7228):490
Clyne AM, Urbano R (2016) An inverted dielectrophoretic device for analysis of attached single cell mechanics. Lab chip 16:561–573
Díaz Lantada A, Alarcon Iniesta H, García-Ruíz JP (2015) Multi-channelled polymeric microsystem for studying the impact of surface topography on cell adhesion and motility. 7(11):2371–2388
Doyle AD, Wang FW, Matsumoto K, Yamada K (2009) One-dimensional topography underlies three-dimensional fibrillar cell migration. J Cell Biol 184(4):481–490
Griep LM, Wolbers F, De Wagenaar B, Ter Braak PM, Weksler BB, Romero IA, Couraud PO, Vermes I, Van der Meer AD, Van den Berg A (2013) BBB on chip: microfluidic platform to mechanically and biochemically modulate blood-brain barrier function. Biomed Microdevices 15(1):205–230
Khodarev NN, Yu J, Labay E, Darga T, Brown CK, Mauceri HJ, Yassari R, Gupta N, Weichselbaum RR (2002) Tumor-endothelium interactions in co-culture: coordinated changes of gene expression profiles and phenotypic properties of endothelial cells. J Cell Sci 116:1013–1022
Kuo CT, Chiang CL, Huang RYJ, Lee H, Wo AM (2012) Configurable 2D and 3D spheroid tissue cultures on bioengineered surfaces with acquisition of epithelial–mesenchymal transition characteristics. NPG Asia Mater 4:e27
Li YS, Haga JH, Chien S (2005) Molecular basis of the effects of shear stress on vascular endothelial cells. J Biomech 38(10):1949–1971
Maher PS, Keatch RP, Donnelly K (2010) Characterisation of rapid prototyping techniques for studies in cell behaviour. Rapid Prototyping J 16(2):116–123
Morss Clyne A, Urbano R (2015) Dielectrophoretic devices for analysis of cell mechanics. US Patent, US 2015/0285760 A1
Rosenthal A, Voldman J (2005) Dielectrophoretic traps for single-particle patterning. Biophys J 88:2193–2205
Tholudur A, Giron L, Alam K, Thomas T, Garr E, Weatherly G, Kulowiec K, Quick M, Shepard S (2006) Comparing automated and manual cell counts for cell culture applications. Bioprocess Int 28–34
Voldman J (2006) Electrical forces for micro-scale cell manipulation. Annu Rev Biomed Eng 8:425–454
Voyvodic PL, Min D, Baker AB (2012) A multichannel dampened flow system for studies on shear-stress mediated mechanotransduction. Lab Chip 12(18):3322–3330
Vunjak-Novakovic C, Scadden DT (2011) Biomimetic platforms for human stem cell research. Cell Stem Cell 8(3):252–261
Wimmer K, Harant H, Reiter M, Blüml G, Gaida T, Katinger H (1994) Two-dimensional gel electrophoresis for controlling and comparing culture supernatants of mammalian cell culture productions systems. Cytotechnology 16(3):137–146
Wong KHK, Chan JM, Kamm RD, Tien J (2012) Microfluidic models of vascular function. Annu Rev Biomed Eng 14:205–230
Yao X, Peng R, Ding J (2013) Cell-material interactions revealed via material techniques of surface patterning. Mater Views 25:5257–5286
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer International Publishing Switzerland
About this chapter
Cite this chapter
Díaz Lantada, A. et al. (2016). Overview of Microsystems for Studying Cell Behavior Under Culture. In: Díaz Lantada, A. (eds) Microsystems for Enhanced Control of Cell Behavior. Studies in Mechanobiology, Tissue Engineering and Biomaterials, vol 18. Springer, Cham. https://doi.org/10.1007/978-3-319-29328-8_12
Download citation
DOI: https://doi.org/10.1007/978-3-319-29328-8_12
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-29326-4
Online ISBN: 978-3-319-29328-8
eBook Packages: EngineeringEngineering (R0)