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Single Molecule Approaches in RNA-Protein Interactions

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RNA Processing

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 907))

Abstract

RNA-protein interactions govern every aspect of RNA metabolism, and aberrant RNA-binding proteins are the cause of hundreds of genetic diseases. Quantitative measurements of these interactions are necessary in order to understand mechanisms leading to diseases and to develop efficient therapies. Existing methods of RNA-protein interactome capture can afford a comprehensive snapshot of RNA-protein interaction networks but lack the ability to characterize the dynamics of these interactions. As all ensemble methods, their resolution is also limited by statistical averaging. Here we discuss recent advances in single molecule techniques that have the potential to tackle these challenges. We also provide a thorough overview of single molecule colocalization microscopy and the essential protein and RNA tagging and detection techniques.

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Abbreviations

CoSMoS:

Colocalization single molecule spectroscopy

EM-CCD:

Electron-multiplied charge-coupled device

HILO:

Highly inclined and laminated optical sheet microscopy

RBP:

RNA-binding protein

RNP:

Ribonucleoprotein

sCMOS:

Scientific complementary metal-oxide-semiconductor

TIRF:

Total internal reflection fluorescence

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Authors and Affiliations

  1. Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA, USA

    Victor Serebrov Ph.D. & Melissa J. Moore Ph.D.

Authors
  1. Victor Serebrov Ph.D.
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  2. Melissa J. Moore Ph.D.
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Correspondence to Melissa J. Moore Ph.D. .

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Editors and Affiliations

  1. Dept of Cellular and Molecular Medicine, Institute for Genomic Medicine, La Jolla, California, USA

    Gene W. Yeo

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Serebrov, V., Moore, M.J. (2016). Single Molecule Approaches in RNA-Protein Interactions. In: Yeo, G. (eds) RNA Processing. Advances in Experimental Medicine and Biology, vol 907. Springer, Cham. https://doi.org/10.1007/978-3-319-29073-7_4

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