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Biosensors for the Detection of Emerging Marine Toxins

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Biosensors for Security and Bioterrorism Applications

Abstract

Emerging marine toxins present in the environment are relevant for food safety issues. Researchers are currently putting special emphasis on the development of biosensors for their detection. Due to their structural complexity and the difficulty to produce the corresponding biorecognition molecules, the development of assays and biosensors for their detection has become a challenge. Compared to traditional detection techniques, biosensors can provide advantages in terms of sensitivity, specificity, design versatility, portability and multiplexed configurations. This chapter provides a critical overview of the immunosensors, receptor-based biosensors, cell-based biosensors and aptasensors that have been developed for the detection of palytoxins (PlTXs), brevetoxins (PbTXs) and tetrodotoxins (TTXs). Although only few biosensors for these emerging marine toxins have been described to date, the chapter reflects the promising advances made in this field.

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References

  1. Anderson PD (2012) Bioterrorism: toxins as weapons. J Pharm Pract 25:121–129

    Google Scholar 

  2. Kruger CL, Reddy CS, Conze DB et al (2014) Food safety and foodborne toxicants. In: Hayes W, Kruger CL (eds) Hayes’ principles and methods of toxicology, 6th edn. CRC Press, Boca Raton, FL, pp 621–675

    Google Scholar 

  3. OPCW (2015) Toxins. Potential chemical weapons from living organisms.http://www.opcw.org/about-chemical-weapons/types-of-chemical-agent/toxins/ . Accessed 28 October 2015

    Google Scholar 

  4. Reverté L, Soliño L, Carnicer O et al (2014) Alternative methods for the detection of emerging marine toxins: biosensors, biochemical assays and cell-based assays. Mar Drugs 12:5719–5763

    Google Scholar 

  5. EFSA (2008) Opinion of the Scientific Panel on Contaminants in the Food chain on a request from the European Commission on marine biotoxins in shellfish – azaspiracids. EFSA J (723): 1-52.

    Google Scholar 

  6. EFSA (2010) Scientific opinion on marine biotoxins in shellfish—emerging toxins: ciguatoxin group. EFSA J 8(6):1627

    Google Scholar 

  7. EFSA (2010) Scientific opinion on marine biotoxins in shellfish: cyclic imines (spirolides, gymnodimines, pinnatoxins and pteriatoxins). EFSA J 8(6):1628

    Google Scholar 

  8. Aligizaki K, Katikou P, Milandri A et al (2011) Occurrence of palytoxin-group toxins in seafood and future strategies to complement the present state of art. Toxicon 57:390–399

    Google Scholar 

  9. Barroso GP, Rueda P, Parron CT et al (2008) An epidemic outbreak with respiratory symptoms in the province of Almeria (Spain) due to toxic microalgae exposure. Gac Sanit 22(6): 578-584

    Google Scholar 

  10. Pfannkuchen M, Godrijan J, Pfannkuchen DM (2012) Toxin-producing Ostreopsis cf. ovata are likely to bloom undetected along coastal areas. Environ Sci Technol 46:5574–5582

    Google Scholar 

  11. EFSA (2009) Scientific opinion on marine biotoxins in shellfish—palytoxin group. EFSA J 7(12):1393

    Google Scholar 

  12. CRLMB (2005) Report on toxicology working group meeting. CRLMB, Cesenatico, Italy, p 24–25

    Google Scholar 

  13. Yakes BJ, Deeds J, White K et al (2011) Evaluation of surface plasmon resonance biosensors for detection of tetrodotoxin in food matrices and comparison to analytical methods. J Agric Food Chem 59:839–846

    Google Scholar 

  14. Campbell K, McNamee SE, Huet AC et al (2014) Evolving to the optoelectronic mouse for phycotoxin analysis in shellfish. Anal Bioanal Chem 406:6867–688

    Google Scholar 

  15. Zamolo VA, Valenti G, Venturelli E et al (2012) Highly sensitive electrochemiluminescent nanobiosensor for the detection of palytoxin. ACS Nano 6:7989–7997

    Google Scholar 

  16. Hilgemann DW (2003) From a pump to a pore: how palytoxin opens the gates. Proc Natl Acad Sci USA 100:386–388

    Google Scholar 

  17. Alfonso A, Pazos MJ, Fernández-Araujo A et al (2014) Surface plasmon resonance biosensor method for palytoxin detection based on Na+, K+-ATPase affinity. Toxins 6:96–107

    Google Scholar 

  18. Volpe G, Cozzi L, Migliorelli D et al (2014) Development of a haemolytic-enzymatic assay with mediated amperometric detection for palytoxin analysis: application to mussels. Anal Bioanal Chem 406:2399–2410

    Google Scholar 

  19. EFSA (2010) Scientific opinion on marine biotoxins in shellfish-emerging toxins: brevetoxin group. EFSA J 8(7):1677

    Google Scholar 

  20. US FDA (United States Food and Drug Administration) (2001) Fish and fisheries products hazards and controls guidance, 3rd edn. Appendix 5—FDA & EPA Safety levels in regulations and guidance. http://www.fda.gov/Food/GuidanceComplianceRegulatoryInformation/GuidanceDocuments/Seafood/ucm091782.htm. Accessed 29 October 2015

    Google Scholar 

  21. NZFSA (New Zealand Food Safety Authority) (2006) Animal products (specification for bivalve molluscan shellfish). http://www.nzfsa.govt.nz/animalproducts/legislation/notices/animal-materialproduct/shellfish/bmsrcsspecv-16_2_signed.pdf . Accessed 29 October 2015

    Google Scholar 

  22. FSANZ (Food Standards Australia New Zealand) (2010) Food standard code, incorporating amendments up to and including amendment 116, standard 4.1.1, primary production and processing standards, preliminary provisions, Standard 1.4.1, contaminants and natural toxicants, issue 111. http://www.foodstandards.gov.au/_srcfiles/Standard_1_4_1_Contaminants_v113.pdf . Accessed 29 October 2015 

    Google Scholar 

  23. Carter RM, Poli MA, Pesavento M et al (1993) Immunoelectrochemical biosensors for detection of saxitoxin and brevetoxin. Immunomethods 3(2):128–133

    Google Scholar 

  24. Kreuzer MP, Pravda M, O’Sullivan CK et al (2002) Novel electrochemical immunosensors for seafood toxin analysis. Toxicon 40:1267–1274

    Google Scholar 

  25. Tang D, Tang J, Su B et al (2011) Gold nanoparticles-decorated amine-terminated poly(amidoamine) dendrimer for sensitive electrochemical immunoassay of brevetoxins in food samples. Biosens Bioelectron 26:2090–2096

    Google Scholar 

  26. Tang J, Hou L, Tang D et al (2012) Magneto-controlled electrochemical immunoassay of brevetoxin B in seafood based on guanine-functionalized graphene nanoribbons. Biosens Bioelectron 38:86–93

    Google Scholar 

  27. Zhang B, Hou L, Tang D et al (2012) Simultaneous multiplexed stripping voltammetric monitoring of marine toxins in seafood based on distinguishable metal nanocluster-labeled molecular tags. J Agric Food Chem 60:8974–8982

    Google Scholar 

  28. Lin Y, Zhou Q, Lin Y et al (2015) Mesoporous carbon-enriched palladium nanostructures with redox activity for enzyme-free electrochemical immunoassay of brevetoxin B. Anal Chim Acta 887:67–74

    Google Scholar 

  29. Tang D, Zhang B, Tang J et al (2013) Displacement-type quartz crystal microbalance immunosensing platform for ultrasensitive monitoring of small molecular toxins. Anal Chem 85:6958–6966

    Google Scholar 

  30. Eissa S, Siaj M, Zoroub M (2015) Aptamer-based competitive electrochemical biosensor for brevetoxin-2. Biosens Bioelectron 69:148–154

    Google Scholar 

  31. Mouri R, Oishi T, Torikai K et al (2009) Surface plasmon resonance-based detection of ladder-shaped polyethers by inhibition detection method. Bioorg Med Chem Lett 19:2824–2828

    Google Scholar 

  32. Wang Q, Fang J, Cao D et al (2015) An improved functional assay for rapid detection of marine toxins, saxitoxin and brevetoxin using a portable cardiomyocyte-based potential biosensor. Biosens Bioelectron 72:10–17

    Google Scholar 

  33. Kulagina NV, Mikulski CM, Gray S et al (2006) Detection of marine toxins, brevetoxin-3 and saxitoxin, in seawater using neuronal networks. Environ Sci Technol 40:578–583

    Google Scholar 

  34. Bane V, Lehane M, Dikshit M et al (2014) Tetrodotoxin: chemistry, toxicity, source, distribution and detection. Toxins 6:693–755

    Google Scholar 

  35. Neagu D, Micheli L, Palleschi G (2006) Study of a toxin-alkaline phosphatase conjugate for the development of an immunosensor for tetrodotoxin determination. Anal Bioanal Chem 385:1068–1074

    Google Scholar 

  36. Taylor AD, Ladd J, Etheridge S et al (2008) Quantitative detection of tetrodotoxin (TTX) by a surface plasmon resonance (SPR) sensor. Sensor Actuat B-Chem 130:120–128

    Google Scholar 

  37. Taylor AD, Vaisocherová H, Deeds J et al (2011) Tetrodotoxin detection by a surface plasmon resonance sensor in pufferfish matrices and urine. J Sens. doi: 10.1155/2011/601704

    Google Scholar 

  38. Vaisocherová H, Taylor AD, Jiang S et al (2011) Surface plasmon resonance biosensor for determination of tetrodotoxin: Prevalidation study. J AOAC Int 94:596–604

    Google Scholar 

  39. Yakes B, DeGrasse S, Poli M et al (2011) Antibody characterization and immunoassays for palytoxin using an SPR biosensor. Anal Bioanal Chem 400:2865–2869 

    Google Scholar 

  40. Campbell K, Barnes P, Haughey SA et al (2013) Development and single laboratory validation of an optical biosensor assay for tetrodotoxin detection as a tool to combat emerging risks in European seafood. Anal Bioanal Chem 405:7753–7763

    Google Scholar 

  41. Yakes B, Kanyuck K, DeGrasse SL (2014) First report of a direct surface plasmon resonance immunosensor for a small molecule seafood toxin. Anal Chem 86:9251–9255

    Google Scholar 

  42. Fomo G, Waryo TT, Sunday CE et al (2015) Aptameric recognition-modulated electroactivity of poly(4-styrenesolfonic acid)-doped polyaniline films for single-shot detection of tetrodotoxin. Sensors 15:22547–22560 

    Google Scholar 

  43. Cheun B, Endo H, Hayashi T et al (1996) Development of an ultra high sensitive tissue biosensor for determination of swellfish poisoning, tetrodotoxin. Biosens Bioelectron 11:1185–1191

    Google Scholar 

  44. Charkhkar H, Knaack GL, Gnade BE et al (2012) Development and demonstration of a disposable low-cost microelectrode array for cultured neuronal network recording. Sensor Actuat B-Chem 161:655–660

    Google Scholar 

  45. Pancrazio J, Gray SA, Shubin YS et al (2003) A portable microelectrode array recording system incorporating cultured neuronal networks for neurotoxin detection. Biosens Bioelectron 18:1339–1347

    Google Scholar 

  46. Wang Q, Su K, Hu L et al (2015) A novel and functional assay for pharmacological effects of marine toxins, saxitoxin and tetrodotoxin by cardiomyocyte-based impedance biosensor. Sensor Actuat B-Chem 209:828–837

    Google Scholar 

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Acknowledgments

The authors acknowledge financial support from the European Union Seventh Framework Programme (FP7/2007-2013) through the ECsafeSEAFOOD project (grant agreement n° 311820) and from the Ministerio de Economía y Competitividad (MINECO) through the DIANA (BIO2011-26311) and the SEASENSING (BIO2014-56024-C2-2-R) projects. Sandra Leonardo and Laia Reverté acknowledge IRTA—Universitat Rovira i Virgili—Banco Santander and the ECsafeSEAFOOD project, respectively, for their PhD grants.

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Correspondence to Mònica Campàs .

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Leonardo, S., Reverté, L., Diogène, J., Campàs, M. (2016). Biosensors for the Detection of Emerging Marine Toxins. In: Nikolelis, D., Nikoleli, GP. (eds) Biosensors for Security and Bioterrorism Applications. Advanced Sciences and Technologies for Security Applications. Springer, Cham. https://doi.org/10.1007/978-3-319-28926-7_11

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