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Single-Shot Phase Shifting Interferometry for Microscopic Measurements of Non-Birefringent Transmissive Phase Samples

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Abstract

Its well known that there is a variety of techniques to obtain n-phase shifts in one shot, and most of them use diffractive elements, holographic ones or pixelated phase masks attached to a CCD camera, among others, to generate from 4 to 9 interferograms simultaneously; nevertheless, some of the components utilized in this arrangements are still expensive, and in order to reduce the cost of operations, we developed an alternative system that does not use diffractive elements, in this research we present a simultaneous phase-shifting interferometer based on polarizing coupled interferometers, this device can be measured the optical phase of non-birefringent microscopic phase samples, generating four interferograms in single capture of the ccd camera with relative phase shifts of π/2. In order to present the capabilities of the system, the results obtained for the phase measurement of Red Blood Cells (RBC) and non-birefringent transparent samples are presented.

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Acknowledgments

This research was supported by the Technological University of Tulancingo, according to The Initiative for the creation of the First Optics and Photonics Engineering undergraduate program in Mexico. Author V. H. Flores Muñoz is grateful to CONACYT for the scholarship provided (Grant. 224506) and Programa para el Desarrollo Profesional Docente (PRODEP) for grant UPBIC-PTC-022. Author N.-I. Toto-Arellano expresses sincere appreciation to Luisa, Miguel and Damian for the support provided. Authors thank M. A. Ruiz for his contribution in proofreading the manuscript.

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Correspondence to V. H. Flores Muñoz .

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Flores Muñoz, V.H., López Ortiz, B., Toto-Arellano, N.I., Martínez-García, A., Rodríguez-Zurita, G. (2017). Single-Shot Phase Shifting Interferometry for Microscopic Measurements of Non-Birefringent Transmissive Phase Samples. In: Martínez-García, A., Furlong, C., Barrientos, B., Pryputniewicz, R. (eds) Emerging Challenges for Experimental Mechanics in Energy and Environmental Applications, Proceedings of the 5th International Symposium on Experimental Mechanics and 9th Symposium on Optics in Industry (ISEM-SOI), 2015. Conference Proceedings of the Society for Experimental Mechanics Series. Springer, Cham. https://doi.org/10.1007/978-3-319-28513-9_31

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  • DOI: https://doi.org/10.1007/978-3-319-28513-9_31

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-28511-5

  • Online ISBN: 978-3-319-28513-9

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