Abstract
The recognition of microorganisms is important in the diagnosing of the micro-biology. In the medical field the cell disorders can affect body tissues indirectly. In this work we use digital holographic microscopy (DHM) that can achieve high contrast images. This method permits us to obtain phase maps by means of digital reconstruction of a wavefront. Phase differences between two states allows us to realize a quantitative analysis of samples.
An off-axis layout is used to determine the morphology of different types of microorganisms. Also, we can directly examine aggregations by corresponding phase maps. The images provide information about the presence or absence of microorganisms that can be used as indicators of the physiological state of the body. By the presented results, we show that DHM may give some new perspectives within biomedical applications.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
M. del Socorro Hernández-Montes, F. Mendoza, C. Pérez, S. Muñoz, J. Esquivel, Digital holographic interferometry applied to the study of tympanic membrane displacements. Opt. Lasers Eng. 49(6), 698–702 (2011)
S. Muñoz, F. Mendoza, M. del Socorro Hernández-Montes, 3D displacement measurements of the tympanic membrane with digital holographic interferometry. Opt. Express 5(20), 5613–5621 (2012)
E. Cuche, P. Marquet, C. Depeursinge, Simultaneous amplitude-contrast and quantitative phase contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. Appl. Opt. 38(34), 6994–7001 (1999)
D. Gabor, Microscopy by reconstructed wave fronts. Proc. R. Soc. A 197, 454–487 (1949)
B. Kemper, G. von Bally, Digital holographic microscopy for live cell applications and technical inspection. Appl. Opt. 47(4), A52–A61 (2008)
C. Maldonado, G. Perdigón, The probiotic bacterium Lactobacillus casei induces activation of the gut mucosal immune system through innate immunity. Clin. Vaccine Immunol. 13(2), 219–226 (2006)
J. Gerritsen, H. Smidt, G.T. Rijkers, W.M. de Vos, Intestinal microbiota in human health and disease: the impact of probiotic. Genes Nutr. 6, 209–240 (2011)
S. Katakweba, S. Mulungu, J. Eiseb, A. Mahlaba et al., Prevalence of haemoparasites, leptospires and coccobacilli with potential for human infection in the blood of rodents and shrews from selected localities in Tanzania, Namibia and Swaziland. Afr. Zool. 47(1), 119–127 (2012)
I. Yamaguchi, J. Kato, S. Otha, J. Mizuno, Image formation in phase-shifting digital holography and applications to microscopy. Appl. Opt. 40, 6177–6186 (2001)
M.K. Kim, Applications of digital holography in biomedical microscopy. J. Opt. Soc. Korea 14(2), 77–89 (2010)
T. Kreis, Digital holographic interference-phase measurement using the Fourier-transform method. J. Opt. Soc. Am. A 3, 847–855 (1986)
M. Takeda, H. Ina, S. Kobayashi, Fourier-transform method of fringe-pattern analysis for computer-based topography and interferometry. J. Opt. Soc. Am. 72, 156–160 (1982)
G. Perdigón, R. Fuller, R. Raya, Lactic acid bacteria and their effect on the immune system. Curr. Issues Intest. Microbiol. 2, 27–42 (2001)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing Switzerland
About this paper
Cite this paper
Solís, S.M., Hernández-Montes, M.d.S., Santoyo, F.M. (2017). Identification of Microorganisms Using Digital Holographic Microscopy. In: Martínez-García, A., Furlong, C., Barrientos, B., Pryputniewicz, R. (eds) Emerging Challenges for Experimental Mechanics in Energy and Environmental Applications, Proceedings of the 5th International Symposium on Experimental Mechanics and 9th Symposium on Optics in Industry (ISEM-SOI), 2015. Conference Proceedings of the Society for Experimental Mechanics Series. Springer, Cham. https://doi.org/10.1007/978-3-319-28513-9_10
Download citation
DOI: https://doi.org/10.1007/978-3-319-28513-9_10
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-28511-5
Online ISBN: 978-3-319-28513-9
eBook Packages: EngineeringEngineering (R0)