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Gene Expression Systems in Fungi: Advancements and Applications pp 23–57Cite as

Production of Industrial Enzymes in Trichoderma reesei

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Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Trichoderma reesei (teleomorph Hypocrea jecorina) is one of the two major fungal platforms for industrial enzyme production, along with Aspergillus sp. Its use derives from its history as a model organism for studies on cellulose degradation and its cellulase enzyme complex since the 1940s, which suggested its use for industrial bioethanol manufacturing during the oil crisis in the mid 1970s. Extensive strain development campaigns by different laboratories proved that the wild type isolate QM6a can be developed into superior production strains, and later the genetic tools were established for the species, which allowed the strains to be tailored for maximum productivity with optimised backgrounds. T. reesei has now maintained its position as a highly productive, easy-to-handle, robust and safe cell factory for more than 40 years. The recently revived interest in lignocellulosic bioethanol paved the way for a comeback in the use of T. reesei native cellulase complex, whereas advances in the development of molecular biology tools—such as bioinformatics and mating—have provided further refinements in the modern strain development of T. reesei for enzyme and other protein production in virtually all segments of industrial biotechnology.

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Notes

  1. 1.

    If 4 g of sugar are consumed for each 1 g of secreted enzyme, 400 g sugar/L would be required to achieve 100 g of secreted enzyme /L (Pourquie et al. 1988; Cherry and Fidantsef 2003).

  2. 2.

    VTT = Technical Research Centre of Finland, Espoo, Finland. ALKO = State Alcohol Monopoly, Helsinki, Finland. In 1995, the enzyme business was moved to Primalco Biotec at the Altia Group, Helsinki, Finland.

  3. 3.

    CAYLA = Société CAYLA, Toulouse, France

  4. 4.

    CETUS = Cetus Corporation, Berkeley, CA, USA

  5. 5.

    Kyowa = Kyowa Hakko Kogyo Co., Ltd., Tokyo, Japan

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Authors and Affiliations

  1. Roal Oy, Tykkimäentie 15b, Rajamäki, 05200, Finland

    Marja Paloheimo M.Sc., Susanna Mäkinen M.Sc. & Jari Vehmaanperä Ph.D.

  2. AB Enzymes GmbH, Feldbergstrasse 78, Darmstadt, 64293, Germany

    Thomas Haarmann Ph.D.

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  1. Marja Paloheimo M.Sc.
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  2. Thomas Haarmann Ph.D.
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  3. Susanna Mäkinen M.Sc.
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  4. Jari Vehmaanperä Ph.D.
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Correspondence to Marja Paloheimo M.Sc. .

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  1. and Environment-Bioresources, AIT Austrian Institute of Technology Department of Health, Tulln, Australia

    Monika Schmoll

  2. and Environment-Bioresources, AIT Austrian Institute of Technology Department of Health, Tulln, Australia

    Christoph Dattenböck

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Paloheimo, M., Haarmann, T., Mäkinen, S., Vehmaanperä, J. (2016). Production of Industrial Enzymes in Trichoderma reesei . In: Schmoll, M., Dattenböck, C. (eds) Gene Expression Systems in Fungi: Advancements and Applications. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-27951-0_2

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