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High-Throughput Construction of Genetically Modified Fungi

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Book cover Gene Expression Systems in Fungi: Advancements and Applications

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Functional studies of fungal genomes are necessary for understanding the fundamentals of eukaryotic genetics and improving human life, as many fungi are economically important for industry, medicine and agriculture. As a prerequisite for functional studies, gene mutagenesis has been actively implemented and many technological advances developed. Prior to the widespread availability of genome sequences, fungal gene mutations were generated using radiation and insertional mutagenesis. Mutations generated using these methods were random and in small scale. With the advent of genome sequences, mutagenesis of fungal genes has been more targeted and carried out in high-throughput manner. Technical improvements in the construction of mutagenesis vectors and increased efficiency of homologous recombination in fungal cells has facilitated the high throughput mutation of fungal genes.

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Authors and Affiliations

  1. Plasma Bioscience Research Center, Kwangwoon University, Seoul, 139-701, Republic of Korea

    Gyungsoon Park

  2. Department of Plant Pathology and Microbiology, University of California, 900 University Avenue, Riverside, CA, 92521, USA

    Shouqiang Ouyang & Katherine A. Borkovich

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  1. Gyungsoon Park
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  2. Shouqiang Ouyang
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  3. Katherine A. Borkovich
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Correspondence to Katherine A. Borkovich .

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Editors and Affiliations

  1. and Environment-Bioresources, AIT Austrian Institute of Technology Department of Health, Tulln, Australia

    Monika Schmoll

  2. and Environment-Bioresources, AIT Austrian Institute of Technology Department of Health, Tulln, Australia

    Christoph Dattenböck

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Park, G., Ouyang, S., Borkovich, K.A. (2016). High-Throughput Construction of Genetically Modified Fungi. In: Schmoll, M., Dattenböck, C. (eds) Gene Expression Systems in Fungi: Advancements and Applications. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-27951-0_10

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