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Characterization and Classification of Stem Cells

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Regenerative Medicine - from Protocol to Patient

Abstract

Starting from a zygote, an organism is made up of thousands, highly organized stem cells, progenitor cells and postmitotic cells which are generated in spatio-temporally coordinated proliferation and differentiation steps. The ongoing advancements in cell culture, isolation techniques, and molecular analyses have driven our basic understanding of different cell types and led to a broad classification of stem cells. This chapter outlines the most prominent techniques used for the characterization and classification of stem cells and provides an overview of many different stem cells, their function and their mRNA, miRNA and protein content.

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Abbreviations

BM:

Bone marrow

BTSC:

Brain tumor stem cell

CB:

Cord blood

CLP:

Common lymphoid progenitor

CMP:

Common myeloid progenitor

CSC:

Cancer stem cell

EPC:

Endothelial progenitor cell

ErP:

Erythroid progenitor

ESC:

Embryonic stem cell

GMP:

Granulocyte-macrophage progenitor

HpSC:

Hepatic stem cell

HSC:

Hematopoietic stem cell

iPSC:

Induced pluripotent stem cell

LT-HSC:

Long-term hematopoietic stem cell

MEP:

Megakaryocyte-erythroid progenitor

MkP:

Megakaryocyte progenitor

MP:

Multipotent progenitors

MSC:

Mesenchymal stem cell

NK:

Natural killer

NSC:

Neural stem cell

PB:

Peripheral blood

RBC:

Red blood cells

SPC:

Spermatogonial progenitor cell

ST-HSC:

Short-term hematopoietic stem cell

TSC:

Tissue stem cell

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  1. Miltenyi Biotec GmbH, Friedrich-Ebert-Street 68, 51429, Bergisch Gladbach, Germany

    Ute Bissels, Yvonne Diener, Dominik Eckardt & Andreas Bosio

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  1. Ute Bissels
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  2. Yvonne Diener
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  4. Andreas Bosio
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Correspondence to Ute Bissels .

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  1. Department of Cardiac Surgery and Refere, University of Rostock, Rostock, Mecklenburg-Vorpomm., Germany

    Gustav Steinhoff

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Bissels, U., Diener, Y., Eckardt, D., Bosio, A. (2016). Characterization and Classification of Stem Cells. In: Steinhoff, G. (eds) Regenerative Medicine - from Protocol to Patient. Springer, Cham. https://doi.org/10.1007/978-3-319-27610-6_1

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