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Protein Complex Production in Alternative Prokaryotic Hosts

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Advanced Technologies for Protein Complex Production and Characterization

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 896))

Abstract

Research for multiprotein expression in nonconventional bacterial and archaeal expression systems aims to exploit particular properties of “alternative” prokaryotic hosts that might make them more efficient than E. coli for particular applications, especially in those areas where more conventional bacterial hosts traditionally do not perform well. Currently, a wide range of products with clinical or industrial application have to be isolated from their native source, often microorganisms whose growth present numerous problems owing to very slow growth phenotypes or because they are unculturable under laboratory conditions. In those cases, transfer of the gene pathway responsible for synthesizing the product of interest into a suitable recombinant host becomes an attractive alternative solution. Despite many efforts dedicated to improving E. coli systems due to low cost, ease of use, and its dominant position as a ubiquitous expression host model, many alternative prokaryotic systems have been developed for heterologous protein expression mostly for biotechnological applications. Continuous research has led to improvements in expression yield through these non-conventional models, including Pseudomonas, Streptomyces and Mycobacterium as alternative bacterial expression hosts. Advantageous properties shared by these systems include low costs, high levels of secreted protein products and their safety of use, with non-pathogenic strains been commercialized. In addition, the use of extremophilic and halotolerant archaea as expression hosts has to be considered as a potential tool for the production of mammalian membrane proteins such as GPCRs.

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Acknowledgments

The authors received funding from the Spanish Ministry of Science and Innovation grant CTQ2015-66206-C2-2-R and the European Community’s Seventh Framework Programme (FP7/2007–2013) for the ComplexINC Project under grant agreement N° 279039.

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  1. Center for Biological Research, Spanish National Research Council (CIB-CSIC), Ramiro de Maeztu 9, 28040, Madrid, Spain

    Sara Gómez, Miguel López-Estepa, Francisco J. Fernández & M. Cristina Vega

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  1. Sara Gómez
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  2. Miguel López-Estepa
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  3. Francisco J. Fernández
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Correspondence to M. Cristina Vega .

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  1. Center for Biological Research, SpanishNationalResearchCouncil(CIB-CSIC), Madrid, Spain

    M. Cristina Vega

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© 2016 Springer International Publishing Switzerland

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Gómez, S., López-Estepa, M., Fernández, F.J., Vega, M.C. (2016). Protein Complex Production in Alternative Prokaryotic Hosts. In: Vega, M. (eds) Advanced Technologies for Protein Complex Production and Characterization. Advances in Experimental Medicine and Biology, vol 896. Springer, Cham. https://doi.org/10.1007/978-3-319-27216-0_8

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