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Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes

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Advanced Technologies for Protein Complex Production and Characterization

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 896))

Abstract

Besides the most established expression hosts, several eukaryotic microorganisms and filamentous fungi have also been successfully used as platforms for the production of foreign proteins. Filamentous fungi and Dictyostelium discoideum are two prominent examples. Filamentous fungi, typically Aspergillus and Trichoderma, are usually employed for the industrial production of enzymes and secondary metabolites for food processing, pharmaceutical drugs production, and textile and paper applications, with multiple products already accepted for their commercialization. The low cost of culture medium components, high secretion capability directly to the extracellular medium, and the intrinsic ability to produce post-translational modifications similar to the mammalian type, have promoted this group as successful hosts for the expression of proteins, including examples from phylogenetically distant groups: humans proteins such as IL-2, IL-6 or epithelial growth factor; α-galactosidase from plants; or endoglucanase from Cellulomonas fimi, among others. D. discoideum is a social amoeba that can be used as an expression platform for a variety of proteins, which has been extensively illustrated for cytoskeletal proteins. New vectors for heterologous expression in D. discoideum have been recently developed that might increase the usefulness of this system and expand the range of protein classes that can be tackled. Continuous developments are ongoing to improve strains, promoters, production and downstream processes for filamentous fungi, D. discoideum, and other alternative eukaryotic hosts. Either for the overexpression of individual genes, or in the coexpression of multiples genes, this chapter illustrates the enormous possibilities offered by these groups of eukaryotic organisms.

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Acknowledgments

We gratefully acknowledge the financial support by Ministerio de Economía y Competitividad (ES) (grants PET2008_0101, BIO2009-11184, BFU2010-22260-C02-02 and CTQ2015-66206-C2-2-R to MCV) and the European Commission (Framework Programme 7) project ComplexINC No. 279039 to MCV.

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  1. Center for Biological Research, Spanish National Research Council (CIB-CSIC), Ramiro de Maeztu 9, 28040, Madrid, Spain

    Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, Teresa Suárez & M. Cristina Vega

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  1. Sara Gómez
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  2. Miguel López-Estepa
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  3. Francisco J. Fernández
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  4. Teresa Suárez
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  5. M. Cristina Vega
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Correspondence to Teresa Suárez or M. Cristina Vega .

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  1. Center for Biological Research, SpanishNationalResearchCouncil(CIB-CSIC), Madrid, Spain

    M. Cristina Vega

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Gómez, S., López-Estepa, M., Fernández, F.J., Suárez, T., Vega, M.C. (2016). Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes. In: Vega, M. (eds) Advanced Technologies for Protein Complex Production and Characterization. Advances in Experimental Medicine and Biology, vol 896. Springer, Cham. https://doi.org/10.1007/978-3-319-27216-0_11

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