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Microbioreactors

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Microsystems for Pharmatechnology

Abstract

In the last decade, microbioreactor (MBR) technology has allowed for rapid advances in biotechnology process development and the investigation of various biological systems from the industrial biotechnology and pharmaceutical biotechnology. Many of the devices that have been reported in the literature are being applied for early-stage bioprocess research. This research makes it possible to perform comprehensive experiments with very expensive substances that are only available in limited quantities.

Microtechnologically fabricated MBRs range in complexity from simple microtiter-based systems to complex automated parallel bioreactors designed to allow meaningful scaling up/scaling down of conventional pilot and large-scale bioprocesses. MBR technology and the capability to monitor cultivation process variables in situ, such as the optical density, dissolved oxygen, pH and fluorescent protein expression, provide real-time and quantitative data from a microliter cultivation broth. Currently, the majority of MBR systems have been designed for batch and fed-batch processing; there are a few efforts directed at developing MBRs for continuous chemostat mode operation.

This overview of microtechnologically fabricated MBRs, their design and application presents the advantages, different strategies for manufacturing and biotechnological applications of these tiny devices in different operation modes. The report discusses the possibility of design versatility and maintaining key aspects, for example, single-use and fluidic connections, as well as the application of MBRs in versatile and different biotechnological fields.

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Acknowledgments

The authors gratefully acknowledge the financial support provided by the German Research Foundation (DFG) in the Research Unit 856 mikroPART—Microsystems for particulate Life-Science-Products at the Technische Universität Braunschweig, Germany. R. Krull has also received funding from the People Programme (Marie Curie Actions, Multi-ITN) of the European Union’s Seventh Framework Programme for research, technological development and demonstration within the project EUROMBR—European network for innovative microbioreactor applications in bioprocess development (Project ID 608104). S. Büttgenbach gratefully acknowledges additional financial support from the Volkswagen Foundation.

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  1. IBVT—Institute of Biochemical Engineering, Technische Universität Braunschweig, Gaußstr. 17, Braunschweig, 38106, Germany

    R. Krull & S. Lladó‐Maldonado

  2. IMT—Institute of Microtechnology, Technische Universität Braunschweig, Alte Salzdahlumer Str. 203, Braunschweig, 38124, Germany

    T. Lorenz, S. Demming & S. Büttgenbach

  3. Global Technology Chemical Intermediates, BASF SE, Ludwigshafen, Germany

    S. Demming

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  1. R. Krull
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  5. S. Büttgenbach
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Correspondence to R. Krull .

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  1. Institute of Microtechnology, Technische Universität Braunschweig, Braunschweig, Germany

    Andreas Dietzel

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Krull, R., Lladó‐Maldonado, S., Lorenz, T., Demming, S., Büttgenbach, S. (2016). Microbioreactors. In: Dietzel, A. (eds) Microsystems for Pharmatechnology. Springer, Cham. https://doi.org/10.1007/978-3-319-26920-7_4

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  • DOI: https://doi.org/10.1007/978-3-319-26920-7_4

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